發(fā)布時(shí)間：2018-12-17 17:46

【摘要】：目的觀察創(chuàng)傷后應(yīng)激障礙(PTSD)模型大鼠海馬、杏仁核神經(jīng)元自噬和凋亡的改變,探討PTSD海馬、杏仁核體積異常的機(jī)制。方法成年健康雄性SD大鼠40只,隨機(jī)分為對(duì)照組和模型組。采用改良的單一連續(xù)應(yīng)激方法制備PTSD大鼠模型;采用Western blotting方法檢測(cè)海馬、杏仁核組織LC-3和Beclin-1的表達(dá)水平;TUNEL染色和流式細(xì)胞術(shù)檢測(cè)海馬、杏仁核神經(jīng)元凋亡情況。結(jié)果模型組海馬、杏仁核組織LC3-Ⅱ/LC3-Ⅰ比值和Beclin-1表達(dá)水平高于對(duì)照組(P0.05);模型組海馬、杏仁核神經(jīng)元TUNEL陽(yáng)性細(xì)胞百分率和凋亡率高于對(duì)照組(P0.01)。結(jié)論 PTSD模型大鼠海馬、杏仁核神經(jīng)元存在明顯的細(xì)胞自噬和凋亡,這可能是海馬、杏仁核體積異常的機(jī)制之一。
[Abstract]:Objective to observe the changes of autophagy and apoptosis of hippocampal and amygdaloid neurons in rats with post-traumatic stress disorder (PTSD) and to explore the mechanism of abnormal volume of hippocampus and amygdala in PTSD. Methods 40 healthy male SD rats were randomly divided into control group and model group. The PTSD rat model was established by modified single continuous stress method. The expression of LC-3 and Beclin-1 in hippocampus and amygdala was detected by Western blotting method. The apoptosis of neurons in hippocampus and amygdala was detected by TUNEL staining and flow cytometry. Results the ratio of LC3- 鈪，

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