【摘要】：目的探討細(xì)胞自噬在孤獨(dú)癥發(fā)病中的作用。方法健康繁殖期Wistar雌鼠20只(250~260g),雄鼠10只(280~290g),按2∶1比例合籠過(guò)夜,次日早上陰道涂片發(fā)現(xiàn)精子計(jì)為孕1d。在其孕12.5d時(shí),10只雌鼠腹腔注射丙戊酸鈉,其子代為孤獨(dú)癥模型組;10只注射同等劑量生理鹽水,其子代為正常組。通過(guò)比較自梳理實(shí)驗(yàn)、三箱實(shí)驗(yàn)驗(yàn)證孤獨(dú)癥模型是否成功;Western blotting方法對(duì)比正常組與孤獨(dú)癥模型組大鼠生后42d(P42)海馬自噬相關(guān)蛋白LC3-Ⅱ、Beclin 1和P62表達(dá)的變化。結(jié)果 1.成功建立孤獨(dú)癥動(dòng)物模型,與正常組比較,自梳理實(shí)驗(yàn)顯示,模型組理毛時(shí)間較正常顯著增加(P0.05),三箱實(shí)驗(yàn)結(jié)果顯示,模型組大鼠交互能力障礙、缺乏對(duì)新鮮事物的偏好性;2.與正常組相比較,模型組大鼠P42d海馬自噬相關(guān)蛋白LC3-Ⅱ表達(dá)顯著降低(P0.05),Beclin 1表達(dá)顯著降低(P0.05),P62表達(dá)顯著升高(P0.05)。結(jié)論孤獨(dú)癥大鼠P42d海馬自噬活性降低,提示增強(qiáng)自噬可能是一個(gè)潛在的治療策略。
[Abstract]:Objective to investigate the role of autophagy in the pathogenesis of autism. Methods 20 Wistar female rats (250 ~ 260g) and 10 males (280-290g) were used to spend the night in a cage in proportion to 2:1. The next morning vaginal smear showed that the sperm was pregnant for 1 day. On the 12th day of pregnancy, 10 female rats were injected intraperitoneally with sodium valproate, 10 rats in the autism model group were injected with the same dose of normal saline, and their children were the normal group. By comparing self-combing experiment and three-box experiment, we verified whether the autistic model was successful or not and whether the expression of LC3- 鈪，

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