【摘要】：背景:神經(jīng)干細(xì)胞移植已應(yīng)用于臨床治療腦癱等腦損傷疾病,但對(duì)老年癡呆的相關(guān)研究并未深入。目的:觀察神經(jīng)干細(xì)胞移植對(duì)老年癡呆大鼠行為學(xué)及免疫調(diào)節(jié)系統(tǒng)的影響。方法:選取SD大鼠35只,切開腦后皮膚,在腦內(nèi)海馬區(qū)注射鵝膏蕈酸,建立老年癡呆模型;另取SD大鼠10只,切開腦后皮膚,在腦內(nèi)海馬區(qū)注射生理鹽水,作為假手術(shù)組。將造模成功的32只老年癡呆大鼠隨機(jī)分為2組,每組16只,實(shí)驗(yàn)組于腦內(nèi)海馬區(qū)注射5×109 L-1的同種異體神經(jīng)干細(xì)胞懸液;模型組僅穿刺,不注射任何物質(zhì),移植后4周,對(duì)3組大鼠進(jìn)行Morris水迷宮實(shí)驗(yàn),連續(xù)5 d;水迷宮實(shí)驗(yàn)1周后,檢測3組大鼠腦組織勻漿白細(xì)胞介素1、白細(xì)胞介素10水平,觀察腦組織病理形態(tài)學(xué)變化。結(jié)果與結(jié)論:1Morris水迷宮實(shí)驗(yàn):模型組認(rèn)知和記憶能力均明顯低于假手術(shù)組(P0.01),實(shí)驗(yàn)組空間學(xué)習(xí)能力、記憶能力均顯著高于模型組(P0.05,P0.01)。2腦組織勻漿炎炎性因子:模型組白細(xì)胞介素1、白細(xì)胞介素10水平明顯高于假手術(shù)組(P0.01),實(shí)驗(yàn)組白細(xì)胞介素1、白細(xì)胞介素10水平明顯低于模型組(P0.01)。3腦組織病理:模型組神經(jīng)元細(xì)胞明顯少于假手術(shù)組,實(shí)驗(yàn)組神經(jīng)細(xì)胞數(shù)目明顯多于模型組。4結(jié)果表明,神經(jīng)干細(xì)胞移植對(duì)老年性癡呆大鼠神經(jīng)元具有補(bǔ)充和保護(hù)作用,可明顯改善動(dòng)物的學(xué)習(xí)和記憶能力。
[Abstract]:Background: neural stem cell transplantation has been applied to the treatment of cerebral palsy and other brain injury diseases. Aim: to observe the effect of neural stem cell transplantation on behavior and immune regulatory system in senile dementia rats. Methods: 35 Sprague-Dawley (SD) rats were selected to cut back the skin of the brain, and the model of senile dementia was established by injecting muscaric acid into the hippocampal area of the brain, and 10 SD rats were selected as sham-operated group, 10 rats were cut off the skin of the brain and the normal saline was injected into the hippocampal area of the brain. Thirty-two senile dementia rats were randomly divided into two groups, 16 rats in each group. The experimental group was injected with 5 脳 109 L-1 neural stem cell suspension in the hippocampal area of the brain, and the model group was only punctured without any injection of any substance at 4 weeks after transplantation. The Morris water maze test was carried out for 5 days, and the levels of interleukin 1 and interleukin 10 in the brain homogenate of the three groups were measured after 1 week of water maze test, and the pathomorphological changes of brain tissue were observed. Results and conclusion: the cognitive and memory abilities of the model group were significantly lower than those of the sham-operated group (P0.01), and the spatial learning ability of the experimental group was significantly lower than that of the control group (P0.01). The level of IL-1 and IL-10 in model group were significantly higher than those in sham-operation group (P0.01). The levels of IL-1 and IL-10 in experimental group were significantly lower than those in model group (P0.05, P0.01), and the levels of IL-1 and IL-10 in model group were significantly higher than those in sham-operated group (P0.01). In the model group (P0.01) .3, the number of neurons in the model group was significantly lower than that in the sham-operated group. The number of nerve cells in the experimental group was significantly higher than that in the model group. The results showed that the neural stem cell transplantation could supplement and protect the neurons of senile dementia rats, and could obviously improve the learning and memory ability of the animals.
【作者單位】： 邯鄲市第一醫(yī)院老年病二科;河北大學(xué)附屬醫(yī)院老年病科;
【基金】：河北省醫(yī)學(xué)科學(xué)研究重點(diǎn)課題(20130921)~~
【分類號(hào)】：R749.16

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