本文選題：環(huán)磷酸腺苷反應(yīng)元件結(jié)合蛋白 + 慢性應(yīng)激�。� 參考：《中國(guó)神經(jīng)精神疾病雜志》2014年01期

【摘要】：目的探討慢性應(yīng)激狀態(tài)下,以及持續(xù)給予鹽酸氟西汀后,大鼠海馬及前額葉部位環(huán)磷酸腺苷反應(yīng)元件結(jié)合蛋白(cAMP-response element binding protein,CREB)水平和磷酸化CREB(p-CREB)表達(dá)的變化。方法成年雄性Sprague-Dawley(SD)大鼠50只隨機(jī)分為三組:應(yīng)激組26只,藥物組12只,對(duì)照組12只。應(yīng)激組與藥物組大鼠均經(jīng)過(guò)8周慢性應(yīng)激刺激,藥物組在第1周開(kāi)始給予鹽酸氟西汀(10mg/g,I.P.),直到8周末。8周后行糖水偏好實(shí)驗(yàn)和曠場(chǎng)實(shí)驗(yàn),根據(jù)8周末糖水偏愛(ài)率將應(yīng)激組大鼠分為應(yīng)激敏感組和應(yīng)激適應(yīng)組。應(yīng)激結(jié)束后將四組大鼠斷頭處死,采用蛋白免疫印記技術(shù)檢測(cè)大鼠海馬、額葉部位CREB和p-CREB水平。結(jié)果 8周末應(yīng)激敏感組糖水偏愛(ài)率低于應(yīng)激適應(yīng)組、藥物組及對(duì)照組(均P0.05);應(yīng)激敏感組的跨格次數(shù)、中心格時(shí)間和直立次數(shù)低于藥物組和對(duì)照組(均P0.05),與應(yīng)激適應(yīng)組無(wú)統(tǒng)計(jì)學(xué)差異(均P0.05)。應(yīng)激敏感組海馬及前額葉中p-CREB水平明顯低于應(yīng)激適應(yīng)組、藥物組及對(duì)照組(均P0.05);四組大鼠海馬部位、前額葉CREB表達(dá)水平無(wú)明顯差異。結(jié)論海馬和額葉部位CREB磷酸化水平可能與應(yīng)激導(dǎo)致的情緒障礙發(fā)生機(jī)制相關(guān)。
[Abstract]:Objective to investigate the level of cyclic adenosine monophosphate (camp) response element binding protein (cAMP-response element binding protein) and the expression of phosphorylated CREB-p-CREBin in hippocampus and prefrontal lobe of rats under chronic stress and after continuous administration of fluoxetine hydrochloride. Methods 50 adult male Sprague-Dawley SD rats were randomly divided into three groups: stress group (n = 26), drug group (n = 12) and control group (n = 12). The rats in the stress group and the drug group were stimulated by chronic stress for 8 weeks. The drug group was given fluoxetine hydrochloride 10 mg / g / g I.P.E at the first week, and the sugar water preference test and open field experiment were performed after the 8th week. Rats in stress group were divided into stress sensitive group and stress adaptation group according to sugar water preference rate at the end of 8 weeks. After the stress, the rats in the four groups were killed and the levels of CREB and p-CREB in hippocampus and frontal lobe were detected by protein immunoimprint technique. Results at the end of 8 weeks, the sugar water preference rate of stress sensitive group was lower than that of stress adaptation group, drug group and control group (all P 0.05). The time of center lattice and the times of standing upright were lower than those of drug group and control group (P 0.05), but there was no significant difference between the control group and the stress adaptation group (all P 0.05). The levels of p-CREB in hippocampus and prefrontal lobe in stress sensitive group were significantly lower than those in stress adaptation group, drug group and control group (all P 0.05). Conclusion the level of CREB phosphorylation in hippocampus and frontal lobe may be related to the mechanism of emotional disorder induced by stress.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬精神衛(wèi)生中心心境障礙科;航天中心醫(yī)院神經(jīng)內(nèi)科;復(fù)旦大學(xué)附屬華山醫(yī)院中西醫(yī)結(jié)合研究所;
【基金】：上海交通大學(xué)科學(xué)基金(編號(hào):11XJ21006) 上海市自然科學(xué)基金(編號(hào):09ZR1427200) 上海市衛(wèi)生局公共衛(wèi)生海外人才項(xiàng)目(編號(hào):GWHW201208) 國(guó)家高技術(shù)研究發(fā)展計(jì)劃“863”計(jì)劃(編號(hào):2006AA02Z430) 國(guó)家自然科學(xué)基金重大計(jì)劃項(xiàng)目(編號(hào):91232719) 國(guó)家臨床重點(diǎn)專科-上海市精神衛(wèi)生中心(編號(hào):衛(wèi)生部醫(yī)政司2011-873)
【分類號(hào)】：R749.4

【參考文獻(xiàn)】

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