發(fā)布時(shí)間：2018-06-02 04:01

  本文選題：天麻素 + 抑郁癥��； 參考：《神經(jīng)解剖學(xué)雜志》2017年02期

【摘要】：目的:探討天麻素對(duì)慢性不可預(yù)見(jiàn)應(yīng)激(CUS)大鼠抑郁樣行為的改善作用及對(duì)炎癥因子IL-1β、IL-6表達(dá)的影響。方法:將40只SD大鼠隨機(jī)分為對(duì)照組(Sham),模型組(CUS),模型(CUS)+天麻素低、中、高劑量組,每組8只。對(duì)照組每天腹腔注射生理鹽水(1 ml/kg),連續(xù)14 d;模型組接受CUS造模,并且在造模結(jié)束后每天腹腔注射生理鹽水(1 ml/kg),連續(xù)14 d;模型+天麻素低、中、高劑量組在CUS造模結(jié)束后每天腹腔注射不同劑量的天麻素(50、100或者200 mg/kg),持續(xù)14 d。隨后,通過(guò)糖水偏好實(shí)驗(yàn)和強(qiáng)迫游泳實(shí)驗(yàn)檢測(cè)各組大鼠的抑郁樣行為,在行為學(xué)檢測(cè)結(jié)束后處死大鼠,通過(guò)ELISA檢測(cè)海馬和血清中IL-1β、IL-6水平變化情況。結(jié)果:(1)慢性不可預(yù)見(jiàn)應(yīng)激(CUS)可以導(dǎo)致明顯的抑郁樣行為,包括糖水偏好減少(P0.05)和強(qiáng)迫游泳不動(dòng)時(shí)間增加(P0.01),CUS組大鼠海馬及血漿的IL-1β、IL-6水平明顯升高(P0.01)。(2)一定劑量(100和200 mg/kg)天麻素干預(yù)可以緩解CUS大鼠的抑郁行為,CUS與CUS+GAS(M)組(P0.05)以及CUS與CUS+GAS(H)組之間(P0.01)存在顯著性差異。(3)中高劑量的天麻素可以恢復(fù)CUS大鼠海馬和血清的IL-1β、IL-6水平,CUS與CUS+GAS(M)組(P0.05)以及CUS與CUS+GAS(H)組之間(P0.05)的IL-1β、IL-6水平存在顯著性差異。結(jié)論:天麻素可以緩解CUS模型大鼠抑郁樣行為,抑制CUS模型大鼠的IL-1β、IL-6水平升高。
[Abstract]:Aim: to investigate the effect of Gastrodin on depressive behavior and the expression of IL-1 尾 -IL-6 in rats with chronic unpredictable stress. Methods: forty Sprague-Dawley rats were randomly divided into two groups: control group (8 rats) with low, medium and high doses of Gastrodin. The control group received intraperitoneal injection of 1 ml / kg saline for 14 days, and the model group received CUS for 14 days, and the model group received intraperitoneal injection of 1 ml / kg saline for 14 days. The high dose group received intraperitoneal injection of different doses of Gastrodin 50100 or 200 mg / kg for 14 days after the end of CUS modeling. Then, the depressive behavior of rats in each group was detected by sugar water preference test and forced swimming test. The rats were killed after behavioral test, and the changes of IL-1 尾 -IL-6 in hippocampus and serum were detected by ELISA. Results: 1) chronic unpredictable stress (CUSS) can lead to significant depressive behavior. Increase of IL-1 尾 -IL-6 in hippocampus and plasma of rats in P0.01CUS group (P 0.05 and 200 mg / kg) Gastrodin intervention can relieve depression behavior of CUS rats (both CUS and CUS GASM) group (P0.05). There was significant difference between CUS and CUS GASH (P 0.01). The middle and high dose of Gastrodin could restore the level of IL-1 尾 -IL-6 in hippocampus and serum of CUS rats. The level of IL-1 尾 -IL-6 in hippocampus and serum of CUS rats was significantly higher than that in group CUS and CUS group (P0.05), and there was significant difference in the level of IL-1 尾 -IL-6 between CUS group and CUS group (P0.05). Conclusion: Gastrodin can alleviate the depressive behavior of CUS rats and inhibit the increase of IL-1 尾 -IL-6 level in CUS rats.
【作者單位】： 第四軍醫(yī)大學(xué)西京醫(yī)院心身科;
【基金】：國(guó)家自然科學(xué)基金(81401109,81571309) 陜西省社會(huì)發(fā)展科技攻關(guān)項(xiàng)目(2015SF005)
【分類號(hào)】：R749.4

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