本文選題：精神分裂癥 + microRNA��； 參考：《醫(yī)學(xué)研究生學(xué)報(bào)》2014年12期

【摘要】：目的目前精神分裂癥的臨床診斷缺乏統(tǒng)一的標(biāo)準(zhǔn)。文中研究精神分裂癥患者血清中差異miRNA表達(dá)譜,為該病的臨床診斷提供依據(jù)。方法采用Flash TagTMBiotin RNA芯片技術(shù)和SAM軟件篩選精神分裂癥患者與健康受試者血清中差異表達(dá)的miRNA,用實(shí)時(shí)熒光定量逆轉(zhuǎn)錄PCR(RT-PCR)進(jìn)行驗(yàn)證。結(jié)果篩選出3個(gè)差異表達(dá)的miRNA,其中hsa-miR-1281上調(diào),表達(dá)倍數(shù)變化為1.508 81;hsa-miR-2861、hsa-miR-638下調(diào),表達(dá)倍數(shù)變化分別為0.641 93和0.516 24。實(shí)時(shí)定量PCR結(jié)果證實(shí),精神分裂癥患者較健康受試者h(yuǎn)sa-miR-2861與hsa-miR-638表達(dá)下調(diào),分別為[(0.037±0.037)vs(3.159±2.761),χ2=4.089,P0.05]及[(0.006±0.006)vs(0.689±0.314),χ2=4.083,P0.05],hsa-miR-1281在精神分裂癥患者組呈高表達(dá)[(1.221±0.041)vs(0.145±0.036),χ2=5.333,P0.05)。結(jié)論精神分裂癥患者血清中存在差異表達(dá)的miRNA譜,miR-1281、miR-2861和miR-638可作為診斷精神分裂癥的一個(gè)新的血清標(biāo)志物。
[Abstract]:Objective at present, the clinical diagnosis of schizophrenia is lack of uniform standard. The differential miRNA expression profile in the serum of schizophrenic patients was studied in order to provide evidence for clinical diagnosis of schizophrenia. Methods Flash TagTMBiotin RNA microarray and SAM software were used to screen the differentially expressed miRNAs in the serum of schizophrenic patients and healthy subjects. Results three differentially expressed miRNAs were screened out, in which hsa-miR-1281 was up-regulated, and the expression multiple was down-regulated by 1.508 81hsa-miR-2861hsa-miR-638, and the expression multiple was 0.641 93 and 0.516 2424 respectively. The results of real-time quantitative PCR showed that the expression of hsa-miR-2861 and hsa-miR-638 were down-regulated in schizophrenic patients (0.037 鹵0.037)vs(3.159 鹵2.761g, 蠂 2 + 4.089U, P 0.05) and [0.006 鹵0.006)vs(0.689 鹵0.314g, 蠂 2 + 4.083P 0.05] hsa-miR-1281 were highly expressed in schizophrenic patients [1.221 鹵0.041)vs(0.145 鹵0.036, 蠂 2 5.333P 0.05, respectively]. Conclusion the differential expression of miR-1281miR-2861 and miR-638 in the serum of schizophrenic patients may be a new serum marker for the diagnosis of schizophrenia.
【作者單位】： 瀘州醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科;
【基金】：四川省教育廳科研項(xiàng)目(11ZB127)
【分類號】：R749.3

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