本文選題：卒中 + 應(yīng)激��； 參考：《浙江大學(xué)》2012年碩士論文

【摘要】：目的 對(duì)比腦缺血與慢性應(yīng)激所致學(xué)習(xí)記憶障礙及海馬病變的強(qiáng)弱,為臨床改善腦卒中后抑郁(Post-stroke depression PSD)提供參考。 實(shí)驗(yàn)方法 40只成年雄性SD大鼠平均分為4組：對(duì)照組、抑郁(應(yīng)激)組、卒中(缺血)組與PSD(缺血加應(yīng)激)組,卒中處理采用改良的選擇性大腦中動(dòng)脈栓塞術(shù)；抑郁處理采用連續(xù)3周的慢性不可預(yù)見(jiàn)性溫和應(yīng)激；Morris水迷宮實(shí)驗(yàn)評(píng)價(jià)依賴海馬的學(xué)習(xí)記憶功能；免疫組織化學(xué)染色及半定量RT-PCR觀察海馬CA3區(qū)腦源性神經(jīng)營(yíng)養(yǎng)因子(Brain-derive neurotrophic factor, BDNF)的表達(dá)變化。 結(jié)果 缺血或應(yīng)激均可使大鼠學(xué)習(xí)功能明顯下降,表現(xiàn)為與同時(shí)點(diǎn)對(duì)照組比較,逃避潛伏期顯著延長(zhǎng),二者的綜合作用更明顯。慢性應(yīng)激對(duì)學(xué)習(xí)功能的影響強(qiáng)于腦缺血損傷。缺血或抑郁減弱記憶功能,但二者的作用差異無(wú)統(tǒng)計(jì)學(xué)意義。與對(duì)照相比,缺血顯著增強(qiáng)海馬CA3區(qū)BDNF的表達(dá)(27.0±2.5與20.1±2.1),應(yīng)激降低BDNF的表達(dá)(15.2±1.8與20.1±2.1),二者綜合作用仍顯著降低BDNF的表達(dá)(8.2±1.5),差異均具有統(tǒng)計(jì)學(xué)意義(,=52.87,P0.05)。 結(jié)論 缺血與慢性應(yīng)激均降低大鼠學(xué)習(xí)記憶功能,應(yīng)激對(duì)認(rèn)知功能的損害高于缺血,而應(yīng)激與缺血的綜合作用對(duì)學(xué)習(xí)記憶損害與抑制BDNF表達(dá)作用更明顯,提示進(jìn)行PSD的綜合治療時(shí),更重視心理社會(huì)應(yīng)激干預(yù)和抑郁狀態(tài)的改善。
[Abstract]:Purpose The learning and memory disorders and hippocampal lesions induced by cerebral ischemia and chronic stress were compared to provide a reference for clinical improvement of post-stroke depression (Post-stroke depression PSDs). Experimental method Forty adult male SD rats were divided into four groups on average: control group, depression group, stroke group and PSD group. The treatment of stroke was treated by modified selective middle cerebral artery embolization. Depression was treated with chronic unpredictable mild stress and Morris water maze for 3 weeks in order to evaluate the learning and memory function of the hippocampus. Immunohistochemical staining and semi-quantitative RT-PCR were used to observe the expression of brain-derived neurotrophic factor, BDNF) in hippocampal CA3. Result Both ischemia and stress could significantly decrease the learning function of rats. Compared with the control group at the same time, the escape latency was significantly prolonged, and the combined effect of the two was more obvious. The effect of chronic stress on learning function is stronger than that on cerebral ischemia. Ischemia or depression weakened memory function, but there was no significant difference between them. Compared with the control group, ischemia significantly enhanced the expression of BDNF in hippocampal CA3 area (27.0 鹵2.5,20.1 鹵2.1), and decreased the expression of BDNF by 15.2 鹵1.8 and 20.1 鹵2.1. The combined effect of ischemia and ischemia still significantly decreased the expression of BDNF (8.2 鹵1.5), and the difference was statistically significant (P 0.05). Conclusion Both ischemic and chronic stress decreased the learning and memory function of rats, and the damage to cognitive function of rats was higher than that of ischemia, while the combined effect of stress and ischemia on learning and memory impairment and inhibition of BDNF expression was more obvious. More attention was paid to psycho-social stress intervention and improvement of depression.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R749.1

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本文編號(hào)：1918137