本文選題：宮內(nèi)發(fā)育遲緩 + 營養(yǎng)干預(yù)��； 參考：《首都醫(yī)科大學(xué)學(xué)報(bào)》2017年06期

【摘要】：目的研究營養(yǎng)干預(yù)對宮內(nèi)發(fā)育遲緩(intrauterine growth retardation,IUGR)大鼠學(xué)習(xí)記憶的影響,探討阿爾茨海默病(Alzheimer's disease,AD)等神經(jīng)變性病可能的發(fā)育起源及營養(yǎng)相關(guān)機(jī)制。方法應(yīng)用熱量限制法制備IUGR大鼠模型。對出生后的對照大鼠(control,C)和IUGR大鼠(I)在哺乳期和斷乳后分別給予自由取食標(biāo)準(zhǔn)飼料(ad libitum food intake,A)、限制取食70%標(biāo)準(zhǔn)飼料(restricted food intake,R)及自由取食高脂飼料(high fat food intake,H)處理,得到6組大鼠:對照自由取食(control with ad libitum food intake,CA)組、對照限制取食(control with restricted food intake,CR)組、對照自由取食高脂(control with high fat food intake,CH)組、IUGR自由取食(IUGR with ad libitum food intake,IA)組、IUGR限制取食(IUGR with restricted food intake,IR)組和IGRU自由取食高脂(IUGR with high fat food intake,IH)組。于新生、斷乳和成年3個不同時期取材,比較各組大鼠體質(zhì)量、腦質(zhì)量、腦/體質(zhì)量比、血糖、血三酰甘油和總膽固醇等指標(biāo)的變化。應(yīng)用Morris水迷宮實(shí)驗(yàn),比較各組成年大鼠空間學(xué)習(xí)記憶能力的變化。結(jié)果新生IUGR大鼠的血糖和總膽固醇濃度與對照組相比降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。斷乳期,血三酰甘油在IA和IH組較正常出生體質(zhì)量組升高(P0.05);血總膽固醇在CR組低于CA組(P0.05),CH、IA和IH組高于CR組(P0.05),IR組低于IA組(P0.05)。成年大鼠血總膽固醇濃度在CH、IA和IH組均高于CA組(P0.05)。斷乳大鼠體質(zhì)量,CH組高于CA組(P0.05),CR、IA和IR組均低于CA組(P0.05);腦質(zhì)量,CR、IA、IH、IR組均低于CA組(P0.05);腦/體質(zhì)量比在CR和IR組高于CA組(P0.05),CH組低于CA組(P0.05)。成年大鼠的腦、體質(zhì)量變化與斷乳時期相似。Morris水迷宮實(shí)驗(yàn)中,撤除平臺后,CH及IR兩組大鼠穿過平臺的次數(shù)均多于CA組(P0.05);CH組大鼠在平臺象限停留時間多于CA、CR和IR組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論出生后的不同營養(yǎng)干預(yù)可造成IUGR大鼠血糖、血脂等血液生物化學(xué)指標(biāo)的變化,伴腦、體質(zhì)量的差異及成年后空間學(xué)習(xí)記憶的改變,提示AD等神經(jīng)變性病的腦內(nèi)變化可能起源于生命早期,并受到生后營養(yǎng)狀況的影響。
[Abstract]:Objective to study the effects of nutritional intervention on learning and memory in intrauterine growth retard rats, and to explore the possible developmental origin and nutrition-related mechanism of neuropathic diseases such as Alzheimer's disease (AD). Methods IUGR rat model was established by caloric restriction method. During lactation and after weaning, the control rats and IUGR rats were treated with free standard feed (ad libitum food intakea), restricted food in taking 70% standard diet and high fat food in take-over (high fat food in takeoff), respectively, during lactation and after weaning. Six groups of rats were obtained: the control with ad libitum food taken in CAA group, the control with restricted food in CRA group, the control with restricted food in the control group, and the control group in the control group, and the control with restricted food in the control group, and the control group in the control group. Compared with the control with high fat food group, the IUGR with ad libitum food in the IUGR-restricted intake group and the IGRU group in the IGRU group were free to eat the IUGR with high fat food in the IUGR group and the IGRU group in the control group. The control group was compared with the control group and the IGRU group in the group of IUGR with ad libitum food in the control group and the IGRU group in the group of IUGR with restricted food in the control group and the group of the IGRU group in the group of IUGR-IUGR with high fat food in the IGRU group. The changes of body weight, brain mass, brain mass ratio, blood glucose, triglyceride and total cholesterol were compared at three different stages of birth, weaning and adulthood. Morris water maze test was used to compare the spatial learning and memory ability of adult rats in each group. Results the concentrations of blood glucose and total cholesterol in neonatal IUGR rats were significantly lower than those in control group (P 0.05). During the weaning period, serum triglyceride was higher in IA and IH group than in normal birth weight group, and total serum cholesterol in CR group was lower than that in CA group (P 0.05) and in IH group was higher than that in CR group (P 0.05) and IR group (P 0.05) lower than that in IA group (P 0.05). The serum total cholesterol concentration of adult rats in CHIA and IH groups was higher than that in CA group (P 0.05). The body weight of the weaned rats in the Ch group was higher than that in the CA group (P 0.05) and in the IR group was lower than that in the CA group (P 0.05), the brain mass ratio in the CR and IR group was lower than that in the CA group (P 0.05), and the brain / body weight ratio in the CR and IR groups was higher than that in the CA group (P 0.05CH). The changes of brain and body mass in adult rats were similar to those in weaning period. In Morris water maze test, the rats of group Ch and IR had more times of passing through the platform than those of group CA (P 0.05). The duration of staying in the platform quadrant of rats in group C was longer than that in group Cacr and group IR. The difference was statistically significant (P 0.05). Conclusion different nutritional interventions after birth can cause changes in blood biochemical indexes such as blood glucose, blood lipid, brain and body mass, and spatial learning and memory in adult IUGR rats. The results suggest that the brain changes of neurodegenerative venereal diseases such as AD may originate from early life and be affected by postnatal nutritional status.
【作者單位】： 首都醫(yī)科大學(xué)燕京醫(yī)學(xué)院生化與分子生物學(xué)教研室;首都醫(yī)科大學(xué)宣武醫(yī)院中心實(shí)驗(yàn)室;
【基金】：北京市自然科學(xué)基金(7132033) 首都醫(yī)科大學(xué)基礎(chǔ)-臨床合作基金(16JL14) 燕京醫(yī)學(xué)院科研基金(15QD02,15QD08,17QD05)~~
【分類號】：R-332;R749.16

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 ;某些因素對大鼠胃肌電的影響[J];基礎(chǔ)醫(yī)學(xué)與臨床;2001年S1期

2 李淑云;簡易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報(bào);2001年04期

3 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡易的大鼠保定方法[J];上海實(shí)驗(yàn)動物科學(xué);2004年01期

4 周劍鋒,李娜,袁發(fā)煥,張耀全;腎間質(zhì)纖維化模型建立中大鼠行為改變的研究[J];中國行為醫(yī)學(xué)科學(xué);2005年04期

5 符路娣;蔡海云;;介紹一種簡便的大鼠灌胃方法[J];中獸醫(yī)學(xué)雜志;2006年03期

6 劉海燕;;灌注固定大鼠的技術(shù)操作及常見問題[J];齊齊哈爾醫(yī)學(xué)院學(xué)報(bào);2006年11期

7 馮雪建;盧占軍;高登慧;;大鼠灌胃給藥方法的研究[J];實(shí)驗(yàn)動物科學(xué)與管理;2006年04期

8 梁秀蘭;阮祥燕;蔣輝;王迎;;SD大鼠絕經(jīng)模型建立的研究[J];醫(yī)學(xué)綜述;2010年09期

9 楊光瑜;周繼紅;尹志勇;張岫竹;張良;劉大維;;低頻共振對大鼠生理功能影響研究[J];醫(yī)用生物力學(xué);2011年02期

10 郭恒怡,劉探娥,曹青,孫瑛,蔡英年,鄧希賢,陳華粹;缺氧敏感性不同的兩種大鼠心、肺組織腎上腺素類受體的比較[J];生理科學(xué);1986年05期

相關(guān)會議論文 前10條

1 櫖蒲生;王日宏;杝灄生;，

本文編號：1849075