本文選題：迷迭香 + 血管性癡呆�。� 參考：《安徽醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的 本實驗采用改良的雙側(cè)頸總動脈結(jié)扎法建立血管性癡呆大鼠模型，觀察迷迭香吸嗅后血管性癡呆大鼠學(xué)習(xí)記憶功能的改善情況；并通過免疫組化法檢測迷迭香吸嗅后實驗大鼠海馬內(nèi)5-HT和GABA含量的變化情況，初步探討迷迭香吸入的作用機制及臨床應(yīng)用價值。 方法 1. VD大鼠模型制備：將健康的SD大鼠用改良的2VO法造成VD模型，即先結(jié)扎左側(cè)頸總動脈，1周后用同樣的方法結(jié)扎右側(cè)頸總動脈。 2.模型大鼠水迷宮篩查：手術(shù)后4周時，初步篩選并存活的模型大鼠16只，正常大鼠和假手術(shù)大鼠各8只。通過Morris水迷宮測定24只大鼠的空間學(xué)習(xí)記憶成績，記錄定位航行實驗中大鼠找到安全平臺的時間（即逃避潛伏期）和空間探索實驗中大鼠穿越原平臺所在位置的次數(shù)（即穿臺次數(shù)）為實驗指標，時間均以120s為限。 3.分組及給藥：將造模成功的大鼠隨機分為模型組和迷迭香組，另設(shè)正常組和假手術(shù)組對照。 （1）迷迭香組（n=8）:每天上、下午分別予以1%濃度的迷迭香吸嗅，每次30min； （2）VD模型組（n=8）：使用蒸餾水替迷迭香精油，其他同迷迭香組； （3）假手術(shù)組（n=8）：假手術(shù)組分離出2側(cè)頸總動脈但不予結(jié)扎，余實驗步驟同VD模型組； （4）正常組（n=8）：不做任何手術(shù)處理，余實驗步驟同模型組。 4.神經(jīng)行為學(xué)和形態(tài)學(xué)觀察①在用藥后5周時，再次使用Morris水迷宮測定大鼠的空間學(xué)習(xí)記憶成績，水迷宮實驗的方法同前。②在神經(jīng)行為學(xué)實驗結(jié)束后，將全部大鼠斷頭取腦，制作石蠟切片，進行HE染色觀察大鼠海馬區(qū)的病理形態(tài)學(xué)變化，再用免疫組化法觀察大鼠海馬區(qū)5-HT和GABA含量的變化情況。 結(jié)果 1.術(shù)后4周時，手術(shù)組的逃避潛伏期明顯長于正常組，差異有統(tǒng)計學(xué)意義(P0.05)；穿臺次數(shù)顯著少于正常組,差異有顯著統(tǒng)計學(xué)意義（P0.05）；假手術(shù)組和正常組相比，潛伏期與穿臺次數(shù)均無明顯差異（P＞0.05）。 2.用藥5周時，，迷迭香吸嗅組的逃避潛伏期短于模型組，差異有統(tǒng)計學(xué)意義(P0.05)；穿臺次數(shù)顯著多于模型組（P0.05）。模型組大鼠的逃避潛伏期明顯長于正常組,差異有顯著統(tǒng)計學(xué)意義（P0.05）；穿臺次數(shù)顯著少于正常組,差異有顯著統(tǒng)計學(xué)意義（P0.05）。 3.免疫組化結(jié)果中，模型組的5-HT和GABA的陽性神經(jīng)元較正常組明顯減少（P0.05），與模型組比較，迷迭香吸嗅組的陽性神經(jīng)元數(shù)明顯增多（P0.05）。 結(jié)論 1.改良的2VO法建立大鼠VD模型，對大鼠腦組織損傷較小且會出現(xiàn)明顯的學(xué)習(xí)記憶障礙，符合VD臨床的特點。 2.迷迭香吸嗅對VD大鼠的學(xué)習(xí)記憶障礙有明顯的改善作用。 3.迷迭香改善VD模型大鼠的學(xué)習(xí)記憶能力可能與增加海馬內(nèi)5-HT和GABA的含量有關(guān)。
[Abstract]:Purpose An improved bilateral common carotid artery ligation method was used to establish vascular dementia rat model and to observe the improvement of learning and memory function of vascular dementia rats after rosemary sniffing. The changes of 5-HT and GABA in hippocampus of rosemary were detected by immunohistochemical method, and the mechanism of rosemary inhalation and its clinical value were discussed. Method 1. Establishment of VD rat model: VD model was established by modified 2VO method in healthy SD rats. The left common carotid artery was ligated for 1 week and then the right common carotid artery was ligated in the same way. 2. Water maze screening in model rats: at 4 weeks after operation, 16 model rats were preliminarily screened and survived, 8 normal rats and 8 sham-operated rats. The spatial learning and memory scores of 24 rats were measured by Morris water maze. The time of finding the safe platform (escape latency) and the times of passing through the position of the original platform (i.e. the number of platform penetration) in the space exploration experiment were recorded as the experimental indexes, and the time limit was 120 s. 3. Group and administration: the rats were randomly divided into model group and rosemary group, and were divided into normal group and sham operation group. Rosemary group (1) rosemary group was given 1% rosemary sniffing for 30 minutes each day and afternoon. Rosemary essential oil was replaced by distilled water and other rosemary groups were treated with distilled water. (3) sham-operation group: two common carotid arteries were isolated but not ligated in sham-operation group, and the remaining experimental steps were the same as VD model group. Normal group: no surgical treatment, the rest of the experimental steps are the same as the model group. 4. Neurobehavioral and morphological observation 1 at 5 weeks after treatment, the spatial learning and memory scores of rats were determined by Morris water maze again. The method of water maze test was the same as that of the previous 2. 2. After the end of neurobehavioral experiment, all the rats were cut off their heads and their brains were removed. The histopathologic changes of hippocampus were observed by HE staining and the changes of 5-HT and GABA in hippocampus were observed by immunohistochemical method. Result 1. At 4 weeks after operation, the escape latency in the operation group was significantly longer than that in the normal group, the difference was statistically significant (P 0.05), and the number of platform piercing was significantly lower than that in the normal group (P 0.05). There was no significant difference between the incubation period and the times of platform penetration (P > 0.05). 2. At 5 weeks, the escape latency of rosemary sniffing group was shorter than that of model group, the difference was statistically significant (P 0.05), and the number of puncture was significantly higher than that of model group (P 0.05). The escape latency of the model group was significantly longer than that of the normal group, and the difference was statistically significant (P 0.05), and the frequency of platform penetration was significantly lower than that of the normal group (P 0.05). 3. The positive neurons of 5-HT and GABA in the model group were significantly lower than those in the normal group, and the number of positive neurons in rosemary sniffing group was significantly higher than that in the model group. Conclusion 1. The modified 2VO method was used to establish VD model in rats. The damage to brain tissue was small and obvious learning and memory disorder appeared, which was in line with the clinical characteristics of VD. 2. Rosemary sniffing can significantly improve learning and memory impairment in VD rats. 3. Rosemary could improve the learning and memory ability of VD rats by increasing the contents of 5-HT and GABA in hippocampus.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R749.13

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