本文選題：重性抑郁障礙 + 長(zhǎng)鏈非編碼RNA�。� 參考：《山西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的 1.建立重性抑郁障礙外周血白細(xì)胞中差異LncRNA/mRNA共表達(dá)網(wǎng)絡(luò)； 2.應(yīng)用生物學(xué)與信息學(xué)手段，篩選抑郁障礙差異表達(dá)的LncRNA并進(jìn)行初步分析。 方法 采用病例-對(duì)照研究，嚴(yán)格按照入排標(biāo)準(zhǔn)，收集病例組和對(duì)照組各10例，控制性別、年齡等因素后，對(duì)病例組和對(duì)照組進(jìn)行一一配對(duì)，采用DSM--IV軸I障礙定式臨床檢查（SCID-I/P)對(duì)重性抑郁障礙患者進(jìn)行篩查診斷，應(yīng)用漢密爾頓抑郁量表(HAMD)進(jìn)行臨床癥狀的評(píng)定,要求病例組符合HAMD21分（17項(xiàng)）或35分（24項(xiàng)）。提取外周血總RNA，應(yīng)用微陣列技術(shù)使用Arrystar公司LncRNA芯片（可同時(shí)檢測(cè)LncRNA/mRNA），檢測(cè)外周血白細(xì)胞中的LncRNA、mRNA并進(jìn)行聚類(lèi)分析,比較病例組和對(duì)照組LncRNA差異表達(dá)的情況，并建立LncRNA/mRNA的共表達(dá)網(wǎng)絡(luò)；其次，查閱文獻(xiàn)報(bào)道抑郁障礙密切相關(guān)的mRNA以及上述方法檢測(cè)出的差異表達(dá)mRNA，結(jié)合芯片篩選出的LncRNA，按照特定基因表達(dá)標(biāo)準(zhǔn)化信號(hào)強(qiáng)度，篩選出差異表達(dá)中起核心調(diào)控作用的LncRNA。使用NimbleScan software (version2.5）對(duì)原始數(shù)據(jù)進(jìn)行預(yù)處理后，采用SPSS17.0軟件包進(jìn)行統(tǒng)計(jì)分析，在實(shí)驗(yàn)組、對(duì)照組先控制性別年齡，采用隨機(jī)方差模型（Random variance model，RVM）校正后進(jìn)行兩分類(lèi)差異基因篩選(Two classification difference genetic screening，TwoclassDif），采用t檢驗(yàn)方法，得到顯著差異表達(dá)的mRNA和LncRNA；對(duì)篩選到的差異mRNA和LncRNA進(jìn)行共表達(dá)（co-expression）分析，以P0.05為差異有統(tǒng)計(jì)學(xué)意義，篩選出在共表達(dá)網(wǎng)絡(luò)中起核心調(diào)控作用的LncRNA，并對(duì)其進(jìn)行初步分析。 結(jié)果 1.重性抑郁障礙患者外周血白細(xì)胞長(zhǎng)鏈非編碼RNA與對(duì)照組相比，差別具有統(tǒng)計(jì)學(xué)意義（P＜0.05），共篩選出2795條差異表達(dá)mRNAs；960條差異表達(dá)的LncRNAs，其中729條表達(dá)上調(diào)、231條表達(dá)下調(diào)，據(jù)此構(gòu)建重性抑郁障礙外周血白細(xì)胞中差異LncRNA/mRNA共表達(dá)網(wǎng)絡(luò)。 2.重性抑郁障礙患者外周血白細(xì)胞中chr1:29493450-29493605chr1:76255317-76255564chr3:47048304-47048512chr5:134693047-134693150chr10:27802593-27802714chr10:103554357-103554675chr11:64534866-64535009chr14:21698593-21698781chrX:108927695-108927824chr20:25670953-25671104chr13113570824-113570935等11條LncRNA的表達(dá)，，差別具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。 結(jié)論 1.重性抑郁障礙患者外周血LncRNA存在差異表達(dá)； 2.11條差異表達(dá)的LncRNA可能與抑郁障礙相關(guān)。
[Abstract]:Purpose 1. To establish the differential LncRNA/mRNA coexpression network in peripheral blood leukocytes of patients with severe depressive disorder. 2. LncRNA differentially expressed in depressive disorder was screened and analyzed by biological and informatics methods. Method A case-control study was used to collect 10 cases each from the case group and the control group in strict accordance with the admission criteria. After controlling for gender and age, the case group and the control group were matched one by one. SCID-I / P) was used to screen and diagnose the patients with severe depressive disorder, and the clinical symptoms were evaluated by Hamilton Depression scale (Hamd). The patients were required to meet the HAMD21 score of 17 items or 35 points to 24 items. Total RNAs in peripheral blood were extracted, and microarray technique was used to detect LncRNA-mRNA-mRNAs (LncRNA-mRNA-mRNAs) in peripheral blood leukocytes, and cluster analysis was carried out to compare the differential expression of LncRNA between the case group and the control group, and to establish the coexpression network of LncRNA/mRNA. Secondly, the mRNA closely related to depression disorder and the differential expression mRNAs detected by the above methods were reviewed. Combined with the LncRNAs screened by the microarray, the LncRNAs which played a central role in the differential expression were screened according to the standardized signal intensity of the specific gene expression. NimbleScan software version 2.5) was used to preprocess the original data, and SPSS17.0 software package was used for statistical analysis. In the experimental group and the control group, the sex and age were controlled first. Random variance model (RVM) was used to screen two classification difference genetic screening genes for two class difons. T test was used to obtain mRNA and LncRNAs that were significantly differentially expressed, and coexpression of mRNA and LncRNA were analyzed. The LncRNAs which play a central role in the coexpression network were screened and analyzed. Result 1. Compared with the control group, the long chain non-coding RNA of peripheral blood leukocytes in patients with severe depressive disorder showed significant difference (P < 0.05). A total of 2795 LncRNASs expressing mRNAs-960 differentially expressed LncRNASS were screened out, and 729 of them up-regulated the expression of mRNAs-960, and down-regulated the expression of LncRNASs. Based on this, the differential LncRNA/mRNA coexpression network in peripheral blood leukocytes of patients with severe depressive disorder was constructed. 2. 閲嶆

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