本文選題：遺忘型輕度認(rèn)知功能障礙 + 腦源性神經(jīng)營(yíng)養(yǎng)因子��； 參考：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年02期

【摘要】：目的:探討快速老化相關(guān)miRNAs在遺忘型輕度認(rèn)知功能障礙(aMCI)患者血清中的表達(dá),闡明其在aMCI發(fā)病中的作用。方法:選取aMCI患者(aMCI組,n=66)和認(rèn)知功能正常老年人(對(duì)照組,n=76)為研究對(duì)象。采用實(shí)時(shí)定量PCR技術(shù)(qRT-PCR)檢測(cè)2組受試者血清中miR-132、miR-193b、miR-130b、miR-20a、miR-296、miR-329和miR-206的表達(dá)。采用TargetScan 6.0軟件對(duì)差異表達(dá)的血清miRNAs進(jìn)行靶基因預(yù)測(cè),并通過(guò)DAVID在線工具分析靶基因生物學(xué)功能;利用酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA)檢測(cè)2組受試者血清中靶基因的水平。結(jié)果:aMCI組患者血清miR-206和miR-132表達(dá)水平均明顯高于對(duì)照組(P0.05),其他miRNAs則差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。靶基因預(yù)測(cè),腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)和沉默信息調(diào)節(jié)因子1(SIRT1)共為miR-206和miR-132的靶基因。aMCI組患者血清BDNF和SIRT1表達(dá)水平均明顯低于對(duì)照組(BDNF:29.50μg·L~(-1)±3.13μg·L~(-1) vs 32.29μg·L~(-1)±3.66μg·L~(-1);SIRT1:1.86μg·L~(-1)±0.25μg·L~(-1) vs 2.10μg·L~(-1)±0.29μg·L~(-1)),2組比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:miR-206和miR-132在aMCI患者血清中表達(dá)水平明顯升高,二者可能通過(guò)調(diào)控其靶基因BDNF和SIRT1的表達(dá)參與aMCI發(fā)病過(guò)程。
[Abstract]:Objective: to investigate the expression of miRNAs in patients with amnesia mild cognitive impairment (MCI) and to elucidate its role in the pathogenesis of aMCI. Methods: the patients with aMCI and the elderly with normal cognitive function (control group) were selected as subjects. The expression of miR-132mmiR-193bgur ~ (-) miR-130b ~ (-) miR-20agur _ (296) miR-299 and miR-206 in the serum of two groups were detected by real-time quantitative PCR technique (qRT-PCR). The expression of miR-329 and miR-206 in the serum of the two groups were detected. The target genes of differentially expressed serum miRNAs were predicted by TargetScan 6.0 software, and the biological functions of target genes were analyzed by DAVID on-line tools, and the target gene levels in serum of the two groups were detected by Elisa. Results the levels of miR-206 and miR-132 in the patients with MCI were significantly higher than those in the control group (P 0.05), but there was no significant difference in other miRNAs between the two groups (P 0.05). 闈跺熀鍥犻嫻，

本文編號(hào)：1810493