本文選題：黃芪注射液 + 白細(xì)胞介素-18��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:選取抑郁障礙建模成功的C57BL/6J型號(hào)小鼠,按照干預(yù)藥物的種類和劑量進(jìn)行分組,比較干預(yù)前后小鼠血清及腦內(nèi)IL-18的水平變化,分析IL-18水平變化與抑郁模型小鼠抑郁行為的相關(guān)性;探索腦內(nèi)及血清IL-18的水平變化方向是否一致;為IL-18能否用于臨床抑郁癥的診斷、病情判斷提供一些基礎(chǔ)研究證據(jù)。方法:1、按照隨機(jī)分組的方法,將60只健康清潔級(jí)C57BL/6J型號(hào)小鼠(購(gòu)于山西醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物中心)按照干預(yù)藥物及劑量的不同,隨機(jī)分為6組,每組10只,分別為(1)健康對(duì)照組(2)抑郁模型對(duì)照組(3)帕羅西汀治療組(4)黃芪注射液高劑量組(5)黃芪注射液中劑量組(6)黃芪注射液低劑量組。2、干預(yù):帕羅西汀干預(yù)組按設(shè)計(jì)劑量5mg/kg/d進(jìn)行灌服,黃芪注射液干預(yù)組按劑量不同分別采取0.025ml/kg/d、0.050ml/kg/d、0.075ml/kg/d(分別相當(dāng)于原生藥2g/kg/d、4g/kg/d、6g/kg/d)進(jìn)行腹腔注射,共干預(yù)56天。對(duì)照組無(wú)藥物干預(yù),其他處理與干預(yù)組相同。在干預(yù)前,采取各小鼠血樣,抑郁模型組經(jīng)過(guò)56天相應(yīng)藥物干預(yù)后,再次采取各組血樣,同時(shí)斷頭處死小鼠,摘取小鼠海馬組織,經(jīng)蠟塊包埋后做切片。用ELISA法測(cè)量小鼠血清IL-18水平,用免疫組化法處理小鼠海馬切片,檢測(cè)小鼠海馬組織中IL-18染色細(xì)胞數(shù)的表達(dá)情況。最后,用SPSS 22.0統(tǒng)計(jì)軟件對(duì)數(shù)據(jù)進(jìn)行分析。結(jié)果:1、抑郁小鼠模型建造結(jié)果模型小鼠在造模28 d后,出現(xiàn)蜷縮少動(dòng)、膽小易驚、毛發(fā)變粗糙、無(wú)光澤、體質(zhì)量增加減慢、攝食減少、糖水消耗量降低,建模成功;2、小鼠血清IL-18檢測(cè)結(jié)果(1)干預(yù)前,與健康對(duì)照組相比,各抑郁模型組小鼠血清IL-18水平明顯升高,差別有統(tǒng)計(jì)學(xué)意義(P0.05);(2)干預(yù)后,各組抑郁模型抑郁模型小鼠血清IL-18水平變化不一,帕羅西汀組、黃芪注射液高劑量組和黃芪注射液中劑量組小鼠血清IL-18水平顯著降低,差別有統(tǒng)計(jì)學(xué)意義(P0.05);而生理鹽水組、黃芪注射液低劑量組小鼠血清IL-18水平變化不明顯,差別無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);3、小鼠海馬IL-18免疫組化結(jié)果各抑郁模型組小鼠在干預(yù)后,海馬染色細(xì)胞數(shù)均高于空白對(duì)照組,差別有統(tǒng)計(jì)學(xué)意義(P0.05),其中帕羅西汀組、黃芪注射液高劑量組和黃芪注射液中劑量組小鼠海馬染色細(xì)胞數(shù)明顯低于生理鹽水組和黃芪注射液低劑量組,差別有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:1、小鼠血清IL-18水平變化可能與其抑郁癥的發(fā)生發(fā)展及轉(zhuǎn)歸相關(guān)。2、各組抑郁模型組小鼠的血清及海馬IL-18水平變化方向一直,血清IL-18水平可以間接反映腦內(nèi)IL-18水平。3、高劑量黃芪注射液和中劑量黃芪注射液均能起到有效的抗抑郁作用,低劑量黃芪注射液抗抑郁作用不明顯。
[Abstract]:Objective: to select C57BL/6J model mice with depressive disorder and group them according to the type and dosage of intervention drugs, and compare the changes of IL-18 levels in serum and brain before and after intervention. To analyze the correlation between the level of IL-18 and depressive behavior in depressive model mice, to explore whether the change direction of IL-18 in brain and serum is the same, and to provide some basic research evidence for the diagnosis of clinical depression and the judgement of the condition of IL-18. Methods: according to the method of random grouping, 60 healthy and clean grade C57BL/6J mice (purchased from Experimental Animal Center of Shanxi Medical University) were randomly divided into 6 groups, 10 mice in each group. 1) healthy control group 2) depression model control group 3) paroxetine treatment group 4) Astragalus injection high dose group 5) Astragalus injection middle dose group 0) Astragalus injection low dose group 2, intervention: paroxetine intervention group was administered with designed dose of 5mg/kg/d. In the astragalus injection intervention group, 0.025 ml / kg / d 0.050 ml / kg / d 0.075 ml / kg / d (equivalent to 2 g / kg / d 4 g / kg / d) of 0.025 ml / kg / d 0.050 ml / kg / d 0.075 ml / kg / d, respectively, were injected intraperitoneally for 56 days. There was no drug intervention in the control group, and other treatments were the same as those in the intervention group. Before intervention, each mouse blood sample was taken. After 56 days of intervention, the depressive model group took each blood sample again, at the same time, the mice were killed at the same time, the hippocampal tissue of the mice was removed, and then embedded with wax block to be sliced. The level of serum IL-18 was measured by ELISA method, and the expression of IL-18 staining cells in the hippocampus of mice was detected by immunohistochemical method. Finally, the data are analyzed with SPSS 22. 0 statistical software. Results: 1. After 28 days of modeling, the model mice developed curling, timid, frightened, coarse hair, dull hair, slow body mass, decreased intake, and decreased consumption of sugar and water. Before the intervention, the serum IL-18 level in the depression model group was significantly higher than that in the healthy control group, and the difference was statistically significant (P 0.05). The levels of serum IL-18 were significantly decreased in paroxetine group, high dose group of Astragalus membranaceus injection and middle dose group of Astragalus membranaceus injection, the difference was statistically significant (P 0.05), while that of normal saline group was significantly lower than that of normal saline group. The level of serum IL-18 in the low dose group of Astragalus membranaceus injection did not change significantly, but there was no significant difference between the two groups. The IL-18 immunohistochemical results of mice hippocampus showed that the number of stained cells in hippocampus of the depression model group was higher than that of the blank control group after intervention. The number of stained cells in hippocampus of paroxetine group, high dose group of astragalus injection and middle dose group of Astragalus membranaceus injection was significantly lower than that of normal saline group and low dose group of astragalus injection, and the difference was statistically significant (P 0.05). Conclusion the changes of serum IL-18 level in mice may be related to the occurrence, development and outcome of depression. The changes of serum and hippocampal IL-18 levels in the depression model group are always in the same direction. The level of serum IL-18 can indirectly reflect the level of IL-18 in brain. High dose Astragalus injection and medium dose Astragalus injection can play an effective antidepressant effect, low dose Astragalus injection has no obvious antidepressant effect.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R749.4;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 張中橋;;血清IL-18是預(yù)測(cè)冠心病重要因子[J];安徽醫(yī)藥;2006年06期

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本文編號(hào)：1810466