本文選題：阿爾茨海默病 + 轉(zhuǎn)基因小鼠��； 參考：《湖南師范大學(xué)自然科學(xué)學(xué)報(bào)》2014年05期

【摘要】：目的:比較研究Aβ在兩種AD轉(zhuǎn)基因小鼠模型腦杏仁核分布的差異.方法:采用18月齡雄性APP/PSl雙轉(zhuǎn)基因(2×Tg-AD)小鼠與同齡同性別APP/PSl/tau三轉(zhuǎn)基因(3×Tg-AD)小鼠,分別進(jìn)行6E10單克隆抗體免疫組化染色等方法顯示Aβ陽(yáng)性神經(jīng)元及斑塊,觀察其分布與形態(tài)等的差異,圖像分析系統(tǒng)定量比較其量的變化.結(jié)果:在杏仁核2×Tg-AD組Aβ陽(yáng)性產(chǎn)物主要位于細(xì)胞外成為細(xì)胞外Aβ(eAβ),形成大量的Aβ陽(yáng)性斑,Aβ陽(yáng)性神經(jīng)元少;而3×Tg-AD組Aβ陽(yáng)性產(chǎn)物主要位于神經(jīng)元細(xì)胞內(nèi),成為細(xì)胞內(nèi)Aβ(iAβ),但Aβ陽(yáng)性斑少見(jiàn).結(jié)論:2×Tg-AD組與3×Tg-AD組Aβ陽(yáng)性產(chǎn)物在杏仁核分布的差異可能反映了兩種AD小鼠模型神經(jīng)病理等改變的不同.
[Abstract]:Objective: to compare the distribution of A 尾 in the amygdala of two AD transgenic mice. Methods: the A 尾 positive neurons and plaques of 18-month-old male APP/PSl double transgenic 2 脳 Tg-AD mice and the same sex APP/PSl/tau tritransgenic 3 脳 Tg-AD mice were detected by 6E10 monoclonal antibody immunohistochemical staining, and the distribution and morphology of A- 尾 -positive neurons and plaques were observed. The change of image analysis system is compared quantitatively. Results: in the 2 脳 Tg-AD group of amygdaloid nucleus, A 尾 -positive products were mainly located in the extracellular A 尾 -tir, and a large number of A 尾 -positive neurons were formed, while in 3 脳 Tg-AD group, A 尾 -positive products were mainly located in the neuronal cells and became intracellular A 尾 -positive spots, but A 尾 -positive spots were rare. Conclusion the difference of the distribution of A 尾 positive products in amygdala between 2 脳 Tg-AD group and 3 脳 Tg-AD group may reflect the difference of neuropathology between the two AD mice.
【作者單位】： 長(zhǎng)沙衛(wèi)生職業(yè)學(xué)院基礎(chǔ)學(xué)科部;湘潭職業(yè)技術(shù)學(xué)院護(hù)理學(xué)院;吉首大學(xué)醫(yī)學(xué)院;中南大學(xué)湘雅醫(yī)學(xué)院;中南大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：湖南省衛(wèi)生廳科研基金課題資助項(xiàng)目(B2014-151) 湖南省教育廳科學(xué)研究資助項(xiàng)目(14C0091) 米塔爾學(xué)生創(chuàng)新資助項(xiàng)目(10MX19) 長(zhǎng)沙市2013年度指導(dǎo)性科技計(jì)劃資助項(xiàng)目(K13ZD046-33)
【分類(lèi)號(hào)】：R749.16

【共引文獻(xiàn)】

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本文編號(hào)：1807652