發(fā)布時間：2018-04-26 20:59

  本文選題：阿爾茨海默病 + 自主跑輪運(yùn)動��； 參考：《神經(jīng)解剖學(xué)雜志》2017年06期

【摘要】：目的:探討自主跑輪運(yùn)動對阿爾茨海默病(Alzheimer’s disease,AD)模型小鼠空間記憶能力、情緒,以及小鼠腦內(nèi)杏仁核NF-κB、TNF-α和IL-10表達(dá)的影響。方法:將18只3月齡的雄性昆明小鼠隨機(jī)分為:二甲基亞砜(DMSO)對照組(n=6)和AD模型組(n=12)。AD模型組小鼠通過側(cè)腦室內(nèi)注射Aβ_(1-42)進(jìn)行造模,造模一周后采用Y迷宮新異臂實(shí)驗(yàn)驗(yàn)證造模是否成功,并將造模成功的AD小鼠隨機(jī)分成AD安靜組(n=6)和AD運(yùn)動組(n=6)。AD運(yùn)動組進(jìn)行為期6周的自主跑輪運(yùn)動,AD安靜組與DMSO對照組在同等環(huán)境下正常飼養(yǎng)6周。運(yùn)動結(jié)束后,采用Y迷宮新異臂實(shí)驗(yàn)、高架十字迷宮實(shí)驗(yàn)、強(qiáng)迫游泳實(shí)驗(yàn)分別檢測三組小鼠的空間記憶能力、焦慮、抑郁情緒,并統(tǒng)計(jì)分析。行為學(xué)實(shí)驗(yàn)結(jié)束后,用蛋白免疫印跡(Western Blot)實(shí)驗(yàn)檢測三組小鼠杏仁核NF-κB、TNF-α和IL-10的表達(dá)變化。結(jié)果:(1)Y迷宮新異臂實(shí)驗(yàn)結(jié)果顯示:造模一周后,AD模型組在新異臂中停留的時間(88.2 s±8.19 s)顯著低于DMSO對照組(135.48 s±8.63 s,P㩳0.01);運(yùn)動結(jié)束后,AD運(yùn)動組在新異臂中停留的時間是AD安靜組的1.7倍(P㩳0.01),但與DMSO對照組無統(tǒng)計(jì)學(xué)差異。(2)高架十字迷宮實(shí)驗(yàn)結(jié)果顯示:AD運(yùn)動組在開放臂中停留的時間較AD安靜組顯著升高(P㩳0.01),但與DMSO對照組無統(tǒng)計(jì)學(xué)差異。(3)強(qiáng)迫游泳實(shí)驗(yàn)結(jié)果顯示:AD運(yùn)動組的不動時間(88.64 s±5.23 s)明顯低于AD安靜組(236.13s±7.04 s,P㩳0.001),但與DMSO對照組無統(tǒng)計(jì)學(xué)差異。(4)蛋白免疫印跡結(jié)果顯示:促炎因子TNF-α和NF-κB在AD安靜組的表達(dá)均顯著高于AD運(yùn)動組和DMSO對照組(P㩳0.05),而抗炎因子IL-10的表達(dá)與之相反,低于AD運(yùn)動組和DMSO組(P㩳0.05),各檢測因子在AD運(yùn)動組和DMSO組的表達(dá)無明顯差異。結(jié)論:自主跑輪運(yùn)動可有效改善AD模型小鼠的空間記憶以及焦慮抑郁情緒,并且改善杏仁核內(nèi)炎性細(xì)胞因子的表達(dá),從而發(fā)揮其腦保護(hù)作用。
[Abstract]:Objective: to investigate the effects of self-running wheel exercise on spatial memory ability, emotion and the expression of NF- 魏 B TNF- 偽 and IL-10 in the amygdala of Alzheimer's disease (AD) mice. Methods: eighteen 3-month-old male Kunming mice were randomly divided into two groups: dimethyl sulfoxide (DMSO) control group (n = 6) and AD model group (n = 12). After a week of modeling, the Y-maze new arm experiment was used to verify whether the model was successful or not. The successful AD mice were randomly divided into AD quiet group and AD exercise group for 6 weeks. The AD quiet group and the DMSO control group were fed in the same environment for 6 weeks. After exercise, Y maze new arms experiment, elevated cross maze test and forced swimming test were used to detect the spatial memory ability, anxiety and depression of the three groups. The expression of NF- 魏 B IL-10 and TNF- 偽 in the amygdala of the three groups were detected by Western blot assay after behavioral experiment. Results the results of the experiment showed that the retention time of AD model group was 88.2 s 鹵8.19 s after the model was made, which was significantly lower than that of the DMSO control group (135.48 s 鹵8.63 s), and that of AD exercise group was AD in the new arm after exercise. The results of the elevated cross maze test showed that the staying time in open arm of the control group was significantly higher than that in the quiet group, but there was no significant difference between the control group and the control group in DMSO. 3) obsessive-compulsion was observed in the control group (P < 0. 01, P < 0. 01, P < 0. 01, P < 0. 01), but no significant difference was found between the control group and the control group (P < 0. 01). The results of swimming test showed that the immobility time of exercise group was 88.64 s 鹵5.23 s), which was significantly lower than that of control group (236.13 s 鹵7.04 s), but there was no significant difference with DMSO control group. 4) the results of Western blot showed that the expression of TNF- 偽 and NF- 魏 B in AD quiet group was higher than that in control group. The expression of anti-inflammatory factor IL-10 was significantly higher than that of AD exercise group and DMSO control group. Compared with AD exercise group and DMSO group, there was no significant difference in the expression of each factor between AD exercise group and DMSO group. Conclusion: the self-running wheel exercise can effectively improve the spatial memory, anxiety and depression of AD mice, and improve the expression of inflammatory cytokines in the amygdala, so as to play a protective role in the brain.
【作者單位】： 山西醫(yī)科大學(xué)人體解剖學(xué)教研室;
【基金】：山西醫(yī)科大學(xué)科技創(chuàng)新基金(C01201002);山西醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院331基金(201213);山西醫(yī)科大學(xué)大學(xué)生創(chuàng)新項(xiàng)目(2010121) 山西省高等學(xué)�？萍柬�(xiàng)目(21021005)
【分類號】：R-332;R749.16
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本文編號：1807632