本文選題：洛伐他汀 + 毒蕈堿型乙酰膽堿受體��； 參考：《中國老年學(xué)雜志》2017年10期

【摘要】：目的觀察洛伐他汀是否對毒蕈堿型乙酰膽堿受體(mAChRs)有上調(diào)作用,探討其是否具有抗β-淀粉樣肽(Aβ)的作用。方法選擇原代培養(yǎng)的大鼠海馬神經(jīng)細胞和SH-SY5Y細胞,采用洛伐他汀和Aβ寡聚體(AβOs)分別或聯(lián)合處理培養(yǎng)細胞24~48 h后,采用蛋白印跡法和實時熒光定量PCR(Real-time PCR)檢測細胞中M1 mAChR和M3 mAChR蛋白及mRNA表達水平。結(jié)果免疫熒光染色顯示原代大鼠海馬神經(jīng)細胞的體外培養(yǎng)純度達85%以上;不同濃度洛伐他汀處理神經(jīng)細胞24 h后,M1 mAChR和M3 mAChR蛋白及mRNA表達水平均明顯高于對照組(P0.05);0.5μmol/L AβOs處理神經(jīng)細胞48 h后,其M1 mAChR和M3 mAChR蛋白及mRNA表達水平均明顯下降(P0.05);但預(yù)先用0.1μmol/L洛伐他汀處理神經(jīng)細胞24 h,可減輕AβOs導(dǎo)致的M1 mAChR和M3 mAChR表達水平下降。結(jié)論洛伐他汀可能具有上調(diào)mAChRs表達水平的作用,對抗Aβ對該受體的作用。
[Abstract]:Aim to observe whether lovastatin can up-regulate muscarinic acetylcholine receptor mAChRsand to explore whether lovastatin can inhibit 尾 -amyloid peptide A 尾.Methods the primary cultured rat hippocampal nerve cells and SH-SY5Y cells were treated with lovastatin and A 尾 oligodeoxynucleotides (A 尾 Os) for 2448 hours, respectively.The expression of M1 mAChR and M3 mAChR protein and mRNA were detected by Western blot and real-time fluorescence quantitative PCR(Real-time PCR.Results Immunofluorescence staining showed that the purity of primary rat hippocampal neurons was more than 85% in vitro.The protein and mRNA levels of M 1 mAChR and M 3 mAChR were significantly higher than those of the control group treated with 0. 05 渭 mol/L A 尾 Os for 48 h after being treated with different concentrations of lovastatin for 24 h.The expression of M1 mAChR and M3 mAChR protein and mRNA decreased significantly, but pretreatment with 0.1 渭 mol/L lovastatin for 24 h could attenuate the decrease of M1 mAChR and M3 mAChR induced by A 尾 Os.Conclusion lovastatin may up-regulate the expression of mAChRs and antagonize the effect of A 尾 on the receptor.
【作者單位】： 貴州醫(yī)科大學(xué)醫(yī)學(xué)分子生物學(xué)重點實驗室;省部共建地方病與少數(shù)民族性疾病教育部重點實驗室;貴州醫(yī)科大學(xué)微生物學(xué)教研室;貴州醫(yī)科大學(xué)附屬醫(yī)院病理科;
【基金】：國家自然科學(xué)基金(81260173) 教育部“長江學(xué)者和創(chuàng)新團隊發(fā)展計劃資助”(IRT13058) 貴州省科技計劃(黔科合重大專項字[2014]6008號) 貴州省創(chuàng)新計劃項目(黔教合協(xié)同創(chuàng)新中心[2014]06)
【分類號】：R749.16
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本文編號：1771685