本文選題：精神分裂癥 + 腫瘤壞死因子　； 參考：《內(nèi)蒙古醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的探討慢性精神分裂癥的腫瘤壞死因子-α（TNF-α）基因啟動子區(qū)的3個(gè)多態(tài)性位點(diǎn)，試圖尋找到精神分裂癥的患病易感基因，為精神分裂癥的病因?qū)W研究提供一些理論上的依據(jù)。方法本研究采用的是病例-對照的關(guān)聯(lián)研究，患者組共篩選出852例診斷為慢性精神分裂癥的患者，正常對照組篩選出519例健康正常人。（1）運(yùn)用PCR/RFLP(Polymerase Chain Reaction-restriction fragment length Polymorphism，聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長度多態(tài)性)檢測患者組和正常對照組血液樣本中的TNF-α啟動子區(qū)-G308A、-C863T和-T1031C這3個(gè)位點(diǎn)的基因型，，比較分析兩組間的等位基因頻率、基因型的分布和單倍型；2、使用SPSS13.0和UNPHASED3.1.4軟件分析收集到的受試對象的數(shù)據(jù)。結(jié)果在基因型分布符合哈伯溫伯格（Hardy-Weinberg）平衡（P0.05）的基礎(chǔ)上：（1）患者組和正常對照組之間-G308A位點(diǎn)的基因型其分布頻率及等位基因其頻率未見統(tǒng)計(jì)學(xué)差異（P0.05）,患者組和正常對照組間的-C863A位點(diǎn)總體基因型頻率有統(tǒng)計(jì)學(xué)差異（χ~2=8.288，P=0.016）,患者組和正常對照組-T1031C位點(diǎn)女性患者其基因型及其等位基因頻率有統(tǒng)計(jì)學(xué)差異（分別為：χ~2=6.112，P=0.047；χ~2=4.54，P=0.033）；（2）總樣本中rs1800629(A)-rs1800630(C)、rs1800630(C)-rs1799964(C)、rs1800629(G)-rs1800630(C)-rs1799964(T)、rs1800629(G)-rs1800630(C)-rs1799964(C)和rs1800629(G)-rs1800630(A)-rs1799964(T)的P值小于0.05。結(jié)論（1）慢性精神分裂癥的TNF-a基因-C863A和-T1031C兩位點(diǎn)基因型與其發(fā)病相關(guān)，但前者的相關(guān)性僅見于總體樣本，后者的相關(guān)性僅見于總體樣本和女性樣本；（2）兩組TNF-α基因的3個(gè)位點(diǎn)單倍型系統(tǒng)的分析結(jié)果如下：rs1800629(A)-rs1800630(C)、rs1800630(C)-rs1799964(C)、rs1800629(G)-rs1800630(C)-rs1799964(T)、rs1800629(G)-rs1800630(C)-rs1799964(C)和rs1800629(G)-rs1800630(A)-rs1799964(T)等,這些單倍型可能與精神分裂癥這種疾病有相關(guān)性。
[Abstract]:Objective to explore three polymorphic loci in the promoter region of tumor necrosis factor- 偽 (TNF- 偽) gene in chronic schizophrenia, and to find out the susceptible gene of schizophrenia, and to provide some theoretical basis for the etiological study of schizophrenia.Methods the study was conducted in a case-control study in which 852 patients diagnosed as chronic schizophrenia were selected from the patient group.PCR/RFLP(Polymerase Chain Reaction-restriction fragment length Polymorphism, polymerase chain reaction-restriction fragment length polymorphism (RFLP) was used to detect TNF- 偽 promoter region G308An-C863T and T1031C in healthy controls.The genotype of the locus,The allele frequency, genotype distribution and haplotype 2 were compared between the two groups. The data were analyzed by SPSS13.0 and UNPHASED3.1.4 software.Results on the basis of genotypic distribution consistent with Hardy-Weinberger balance (P0.05)), there was no statistical difference in genotype frequency and allele frequency between patient group and normal control group (P 0.05), and between patient group and normal control group, there was no statistical difference in the distribution frequency and allele frequency of G308A locus.鐓х粍闂寸殑-C863A浣嶇偣鎬諱綋鍩哄洜鍨嬮鐜囨湁緇熻瀛﹀樊寮

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