本文選題：阿爾茨海默病　切入點(diǎn)：細(xì)胞周期　出處：《中風(fēng)與神經(jīng)疾病雜志》2014年09期

【摘要】：目的研究阿爾茨海默病(Alzheimer’s disease,AD)患者外周血淋巴細(xì)胞細(xì)胞周期分布及周期素依賴性蛋白激酶抑制劑p27(p27)、細(xì)胞周期素E(cyclin E)表達(dá)的改變及意義。方法選取40例AD患者和30例正常對(duì)照者外周血,立即用密度梯度離心法分離淋巴細(xì)胞,用流式細(xì)胞分析儀檢測(cè)淋巴細(xì)胞細(xì)胞周期分布,分析DNA相對(duì)含量(DNA relative contents,DNARC)和細(xì)胞增殖指數(shù)(cell proliferation index,PI),用免疫印跡法檢測(cè)淋巴細(xì)胞p27、cyclin E的表達(dá)水平。結(jié)果與正常對(duì)照組相比,AD組外周血淋巴細(xì)胞分布于G1期的比例(G1%)顯著下降而S%顯著升高(P0.01);淋巴細(xì)胞DNARC和PI顯著升高(P0.01);p27在AD組表達(dá)顯著下降(P0.01)。輕、中重度AD組之間外周血淋巴細(xì)胞周期的改變及p27水平無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論 AD早期外周血淋巴細(xì)胞即顯示了增強(qiáng)的增殖能力和細(xì)胞周期調(diào)節(jié)的紊亂,且與病情進(jìn)展無關(guān),淋巴細(xì)胞p27水平有可能成為AD早期診斷的外周標(biāo)志物。
[Abstract]:Objective to study the cell cycle distribution of peripheral blood lymphocytes and the expression of cyclin-dependent protein kinase inhibitor p27p27, cyclin E(cyclin in patients with Alzheimer's disease (AD). Methods 40 patients with AD and 30 patients with AD were enrolled in this study. Peripheral blood of normal controls, Lymphocytes were separated by density gradient centrifugation and the cell cycle distribution was detected by flow cytometry. The relative content of DNA relative contentsDNA) and cell proliferation index (Pi) were analyzed. The expression of p27 cyclin E in lymphocytes was detected by Western blotting. Results compared with the control group, the percentage of peripheral blood lymphocytes in G 1 phase in AD group was significantly higher than that in control group (P < 0.05). The expression of DNARC and Pi in lymphocytes decreased significantly in AD group and decreased significantly in AD group. There was no significant difference in the changes of lymphocyte cycle and the level of p27 between moderate and severe AD patients. Conclusion Peripheral blood lymphocytes in the early stage of AD showed increased proliferative ability and disorder of cell cycle regulation, which was not related to the progression of the disease. The level of lymphocyte p27 may be a peripheral marker for early diagnosis of AD.
【作者單位】： 河北醫(yī)科大學(xué)第二醫(yī)院神經(jīng)內(nèi)科;河北省神經(jīng)病學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：河北省自然基金資助項(xiàng)目(C2011206110)
【分類號(hào)】：R749.16

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