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  本文關鍵詞： 血栓心脈寧 老年性癡呆 β-淀粉樣蛋白 大鼠 Wistar　出處：《吉林大學學報(醫(yī)學版)》2014年05期 　論文類型：期刊論文

【摘要】：目的:觀察血栓心脈寧對β-淀粉樣蛋白(Aβ)致老年性癡呆(AD)大鼠行為學及大腦皮層和海馬組織結(jié)構(gòu)的影響,探討血栓心脈寧對大鼠AD的治療作用。方法:選取100只雄性Wistar大鼠,按體質(zhì)量將大鼠隨機分為假手術組、模型組、陽性藥組(鹽酸多奈哌齊,1.75mg·kg-1)和血栓心脈寧1.1、2.2g·kg-1劑量組。連續(xù)灌胃給藥15d。大鼠海馬定位注射Aβ建立大鼠AD模型。進行Morris水迷宮試驗、避暗及病理學實驗。結(jié)果:水迷宮試驗,與模型組比較,血栓心脈寧1.1g·kg-1劑量組大鼠第2、4和5天到達平臺的潛伏期和游程明顯縮短(P0.05或P0.01);血栓心脈寧2.2g·kg-1劑量組大鼠第3~6天到達平臺的潛伏期縮短(P0.05或P0.01),第3~5天到達平臺的游程明顯縮短(P0.05或P0.01);第7天血栓心脈寧各劑量組大鼠在90s內(nèi)經(jīng)過平臺的次數(shù)、平臺停留時間、平臺區(qū)停留距離、有效區(qū)停留時間、有效區(qū)停留距離、平臺停留時間/總時間、平臺停留距離/總路程和有效區(qū)停留時間/總時間均明顯增加(P0.05或P0.01)。避暗實驗中,與模型組比較,血栓心脈寧各劑量組大鼠第2天的錯誤潛伏期及錯誤次數(shù)無顯著差異(P0.05)。病理學觀察,血栓心脈寧各劑量組大鼠與模型組比較其病理學表現(xiàn)無明顯差異,大腦皮層神經(jīng)細胞有變性、壞死;海馬神經(jīng)細胞數(shù)少,層次不清楚,可見變性、壞死細胞。結(jié)論:血栓心脈寧可改善AD大鼠的學習記憶功能,但對其病理學病變無明顯改善作用。
[Abstract]:Objective: to observe the effect of Xuexinmaining on the behavior, cerebral cortex and hippocampal tissue structure of AD rats induced by 尾 -amyloid protein A 尾, and to explore the therapeutic effect of Xuexinmaining on AD in rats. Methods: 100 male Wistar rats were selected. Rats were randomly divided into sham-operation group, model group, positive drug group (Donepezil hydrochloride 1.75mg 路kg-1) and thromboxinine 1.1g 路kg-1 group according to body mass. The rats were injected with A 尾 in hippocampus for 15 days to establish AD model. Morris water maze test was performed. Dark avoidance and pathological experiments. Results: water maze test, compared with the model group, In Xuexinmaining 1.1g 路kg-1 group, the latency and travel time to the platform were significantly shortened on the 4th and 5th day, P0.05 or P0.01, respectively, while the incubation period of Xuexinmaining 2.2g 路kg-1 group was shortened by P05 or P0.01a on the 6th day, and reached the platform on the 35th day by the dose of Xuexinmaining (2.2g 路kg-1). On the 7th day, the rats of each dose group of Xuexin Maining passed through the platform within 90 s, the course was significantly shorter than that of the control group (P 0.05 or P 0.01). Platform stay time, platform area stay distance, effective area stay time, effective area stay distance, platform stay time / total time, The length of stay / total distance of platform and the residence time of effective area / total time were significantly increased by P0.05 or P0.01. in the dark avoidance experiment, compared with the model group, There was no significant difference in the error latency and the number of errors in the rats of each dose group of Xuexinmaining on the second day (P 0.05). There was no significant difference in the pathological manifestations of the rats in each dose group of Xuexinmaining compared with the model group, and the pathological changes of the rats in each dose group were similar to those in the model group. The number of hippocampal neurons is small, the level is unclear, the degeneration and necrosis cells can be seen. Conclusion: Xuexinmai can improve the learning and memory function of AD rats. However, there was no obvious improvement on pathological changes.
【作者單位】： 吉林大學藥學院藥理學教研室;
【基金】：吉林省中醫(yī)藥管理局科研基金資助課題(2010-115)
【分類號】：R749.16

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