本文關(guān)鍵詞： 白藜蘆醇 脂多糖 PC12細(xì)胞 RT-PCR 炎性細(xì)胞因子　出處：《昆明醫(yī)科大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過(guò)構(gòu)建脂多糖(Lipopolysaccharide, LPS)誘導(dǎo)的大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞株P(guān)C12細(xì)胞炎癥模型,建立阿爾茨海默病的體外模型；從細(xì)胞、分子層面探討白藜蘆醇(Resveratrol,RES)對(duì)PC12細(xì)胞炎癥的保護(hù)作用。方法：體外培養(yǎng)的PC12細(xì)胞隨機(jī)分成空白對(duì)照組、LPS (1mg/ml,18h)處理組、不同濃度的白藜蘆醇(25、50和100μM,1h)+LPS處理組。采用免疫熒光雙標(biāo)記檢測(cè)方法(Double-immunofluorescence Labeling)在熒光顯微鏡下觀察細(xì)胞內(nèi)白介素-1β(interleukin-1β, IL-1β)和腫瘤壞死因子-α(tumor necrosis factor-α, TNF-α)的表達(dá)變化；應(yīng)用逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(reverse transcription polymerase chain reaction, RT-PCR)在mRNA水平檢測(cè)TNF-α和IL-1p的表達(dá)變化。 結(jié)果：不同濃度的白藜蘆醇(25、50和100μM)在翻譯和轉(zhuǎn)錄水平明顯抑制了LPS誘導(dǎo)的PC12細(xì)胞炎性細(xì)胞因子IL-1β和TNF-α的表達(dá),并呈現(xiàn)濃度依賴性抑制。 結(jié)論：白藜蘆醇能夠抑制脂多糖誘導(dǎo)的PC12細(xì)胞炎性細(xì)胞因子IL-1β和TNF-α的釋放,從而發(fā)揮其抗神經(jīng)炎癥的作用。
[Abstract]:Objective: to establish an in vitro model of Alzheimer's disease in rat adrenal pheochromocytoma cell line PC12 cells induced by lipopolysaccharide (LPS). To investigate the protective effect of resveratrol resveratrolon resveratrol on inflammation of PC12 cells at the molecular level. Methods: PC12 cells cultured in vitro were randomly divided into two groups: the control group treated with 1 mg / ml of resveratrol for 18 h. The expression of interleukin-1 尾 interleukin-1 尾 interleukin-1 尾 (IL-1 尾) and tumor necrosis factor- 偽 (TNF- 偽) were observed under fluorescence microscope in different concentration groups treated with resveratrol 2550 and 100 渭 m for 1 h) LPS. Reverse transcription polymerase chain reaction (RT-PCRR) was used to detect the expression of TNF- 偽 and IL-1p at mRNA level. Results: different concentrations of resveratrol 2550 and 100 渭 M inhibited the expression of PC12 inflammatory cytokines IL-1 尾 and TNF- 偽 in a dose-dependent manner at the translation and transcription levels. Conclusion: resveratrol can inhibit the release of inflammatory cytokines IL-1 尾 and TNF- 偽 in PC12 cells induced by lipopolysaccharide, thus exerting its anti-neuritis effect.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R749.16

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 林允信;許昌泰;;阿爾茨海默病流行病學(xué)和相關(guān)基因研究現(xiàn)狀[J];醫(yī)學(xué)綜述;2012年11期

，

本文編號(hào)：1517629