本文關(guān)鍵詞： 阿爾茨海默病 膽堿能損傷 蛋白質(zhì)組學(xué) 神經(jīng)元烯醇化酶　出處：《中國(guó)老年學(xué)雜志》2014年14期 　論文類型：期刊論文

【摘要】：目的探討阿爾茨海默病(AD)模型大鼠腦組織中的蛋白表達(dá)變化。方法實(shí)驗(yàn)選取Wistar雄性成年大鼠20只,隨機(jī)分為正常組及AD模型組。切斷大鼠雙側(cè)海馬制備AD動(dòng)物模型。肽指紋圖譜是通過NCBI對(duì)特定差異蛋白點(diǎn)進(jìn)行生物質(zhì)譜分析、鑒定。對(duì)不同實(shí)驗(yàn)組海馬蛋白進(jìn)行測(cè)定。通過蛋白變化的比較,選取有差異的蛋白點(diǎn)條件:AV Ratio1.3或1.3,t檢驗(yàn)P0.05,為有意義蛋白點(diǎn)。結(jié)果進(jìn)一步通過SwissPort蛋白數(shù)據(jù)庫進(jìn)行鑒定。結(jié)果質(zhì)譜分析發(fā)現(xiàn)AD模型組海馬內(nèi)有一表達(dá)明顯升高的蛋白點(diǎn),經(jīng)鑒定為神經(jīng)元烯醇化酶(NSE)。結(jié)論 NSE在AD的病程中,表達(dá)發(fā)生變化,是在AD疾病中起重要作用的酶。
[Abstract]:Objective to investigate the changes of protein expression in the brain of Alzheimer's disease (AD) rats. Methods Twenty adult Wistar male rats were selected. The animal model of AD was made by cutting off the bilateral hippocampus of rats. The peptide fingerprint was used to analyze the specific differential protein sites by NCBI. Identification. Determination of hippocampal proteins in different experimental groups. Different protein point conditions were selected, such as: AV Ratio1.3 or 1.3t test (P0.05). The results were further identified by SwissPort protein database. Results A significantly increased protein expression was found in the hippocampus of AD model group by mass spectrometry analysis. Conclusion the expression of NSE changes in the course of AD, and it is an important enzyme in AD disease.
【作者單位】： 河北大學(xué);吉林大學(xué)第一臨床醫(yī)院神經(jīng)內(nèi)科;河北大學(xué)附屬醫(yī)學(xué);
【基金】：吉林省科技廳課題(200505204;200705129) 河北省醫(yī)學(xué)科學(xué)研究重點(diǎn)發(fā)展計(jì)劃(20110520) 河北大學(xué)附屬醫(yī)院博士基金(310103030929)
【分類號(hào)】：R749.16

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