本文關(guān)鍵詞： 帕金森病 亞型 抑郁 Tef rs738499 基因多態(tài)性　出處：《青島大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的探討中國(guó)北方漢族人群Tef rs738499基因多態(tài)性與帕金森病不同亞型合并抑郁的相關(guān)性。方法采用病例對(duì)照研究方法,選取230例帕金森病患者(PD組)和250例同期健康查體者(對(duì)照組),并根據(jù)24項(xiàng)版本的漢密爾頓抑郁量表(Hamilton rating scale for depresssion,HAMD)評(píng)分將PD患者分為抑郁組(PDD組,n=116)和非抑郁組(non-PDD,n=114)。PDD組患者年齡44-82歲,其中男性58人(50.0%),non-PDD組患者年齡45-81歲,其中男性58人(50.9%)。對(duì)照組患者共250例,年齡34-89歲,其中男性134人(53.6%)。根據(jù)運(yùn)動(dòng)癥狀及統(tǒng)一帕金森病評(píng)估量表第三部分(Unified Parkinson’s Disease Rating Scale-PartⅢ,UPDRS-Ⅲ)評(píng)分將PD組進(jìn)一步分為震顫型(n=102)、姿勢(shì)障礙型(n=105)、不確定型(n=23)3種亞型。應(yīng)用聚合酶鏈反應(yīng)-限制性?xún)?nèi)切酶多態(tài)性(Polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法檢測(cè)Tef rs738499等位基因和基因型分布,并進(jìn)行測(cè)序驗(yàn)證。采用SPSS 17.0(SPSS 18.0,Chicago,USA)軟件進(jìn)行統(tǒng)計(jì)分析。結(jié)果(1)PDD組和n-PDD組在年齡、病程、UPDRS-Ⅲ評(píng)分上均存在顯著差異(均P0.05),而在性別無(wú)顯著差異;(2)Tef rs738499位點(diǎn)的基因型有3種,分別是TT、TG、GG。該基因型在PDD組、n-PDD組、對(duì)照組的分布經(jīng)檢驗(yàn)均符合Hardy-Weinberg平衡(P0.05)。(3)與對(duì)照組相比,該位點(diǎn)T等位基因在PD組中差別無(wú)統(tǒng)計(jì)學(xué)意義(OR:0.983 95%CI:0.720~1.342 P=0.913),野生型基因型即TT基因型在PD組中差別無(wú)統(tǒng)計(jì)學(xué)意義(OR:0.931 95%CI:0.646~1.340 P=0.699);將PD患者分為PDD與n-PDD組后,T等位基因在兩組之間比較差別有統(tǒng)計(jì)學(xué)意義,T等位基因發(fā)生PDD的風(fēng)險(xiǎn)較n-PDD組是G等位基因的3.0倍(OR:2.975 95%CI:1.838~4.815 P0.01),野生型基因型在兩組之間比較差別有統(tǒng)計(jì)學(xué)意義,TT基因型發(fā)生PDD的風(fēng)險(xiǎn)是TG+GG型的4.6倍(OR:4.646 95%CI:2.637~8.186 P0.01);(4)帕金森病患者亞型分析顯示:該位點(diǎn)TT基因型在震顫型、姿勢(shì)障礙型、不確定型3種亞型中,抑郁組與非抑郁組比較均有顯著差異;(5)多因素Logistic回歸分析顯示:病程、UPDRS-Ⅲ評(píng)分、Tef rs738499的TT基因型是PDD的獨(dú)立危險(xiǎn)因素。Tef rs738499的TT基因型攜帶者罹患PDD的風(fēng)險(xiǎn)顯著增高;病程越長(zhǎng),UPDRS-Ⅲ評(píng)分越高,罹患PDD的風(fēng)險(xiǎn)越高。結(jié)論(1)Tef基因rs738499位點(diǎn)多態(tài)性與帕金森病沒(méi)有關(guān)聯(lián)。(2)Tef基因rs738499位點(diǎn)多態(tài)性與帕金森病合并抑郁相關(guān)。(3)帕金森病亞型分析:Tef基因rs738499位點(diǎn)帕金森病抑郁組與非抑郁組在震顫型、姿勢(shì)障礙型、不確定型3種亞型中的分布均有顯著差異。(4)病程、UPDRS-Ⅲ評(píng)分、Tef rs738499的TT基因型是PDD的獨(dú)立危險(xiǎn)因素。
[Abstract]:Objective to investigate the association of Tef rs738499 gene polymorphism with different subtypes of Parkinson's disease (PD) associated with depression in Han population in northern China. Methods A case-control study was conducted. Two hundred and thirty patients with Parkinson's disease (PD) and 250 healthy controls (control group) were selected. According to 24 items of Hamilton rating scale for depresssion. The patients with PD were divided into depression group (PDD) group (n = 116) and non-depression group (n = 114). The patients in the PDD group were aged 44-82 years. The age of the patients in the non-PDD group was 45-81 years old, and 50.9 in the male group. 250 patients in the control group were aged 34-89 years. According to the motor symptoms and the Unified Parkinson's Disease Assessment scale, part 3 (. Unified Parkinson's Disease Rating Scale-Part 鈪，

本文編號(hào)：1468734