本文關(guān)鍵詞：髓過氧化物酶基因多態(tài)性與血管性癡呆相關(guān)性研究　出處：《新鄉(xiāng)醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的探討人群中髓過氧化物酶基因-63GA和-638CA多態(tài)性與血管性癡呆相關(guān)性。 方法年齡、性別方面相匹配的271例血管性癡呆患者和211例健康體檢者,應(yīng)用等位基因特異引物PCR (ASP-PCR)技術(shù),檢測(cè)兩組被檢者M(jìn)PO基因-463GA和-638CA多態(tài)位點(diǎn)的基因型和等位基因的分布頻率。采用Hardy-Weinberg平衡法檢驗(yàn)樣本基因型的吻合度,SPSS18.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果 1研究樣本人群的MPO基因-463GA和-638CA具有多態(tài)性。MPO基因-463GA多態(tài)位點(diǎn)的等位基因分布頻率為,正常對(duì)照組：G為0.762,A為0.238,血管性癡呆組：G為0.779,A為0.221, MPO基因-638CA的等位基因頻率為,正常對(duì)照組：C為0.846,A為0.154,血管性癡呆組：C為0.812,A為0.188,兩組比較沒有顯著性差異(P0.05)。Logistic回歸分析顯示,-463GA和-638CA多態(tài)位點(diǎn)的基因型對(duì)血管性癡呆患病風(fēng)險(xiǎn)沒有影響(P0.05)。 2血管性癡呆組的“-463GG”型亞組中,攜帶'-638CA+AA"基因型受試者,高于“-638CC”基因型攜帶者的糖尿病患病率(P0.05)；而‘'-463GA+AA"型亞組中,兩者糖尿病的發(fā)病率比較,差異無顯著性(P0.05)。血管性癡呆組的-463位點(diǎn)兩不同基因型亞組中,-638位點(diǎn)不同基因型的高血壓患病率沒有明顯差異(／0.05)。 結(jié)論 1MPO基因-463GA和-638CA多態(tài)性,與血管性癡呆的發(fā)生風(fēng)險(xiǎn)無明顯相關(guān)性。 2MPO基因-463GA與-638CA多態(tài)性,一定程度上影響血管性癡呆患者的患糖尿病風(fēng)險(xiǎn)。
[Abstract]:Objective To explore the correlation between the polymorphism of myeloperoxidase gene - 63GA and -638CA in the population and vascular dementia . Methods The distribution frequencies of the genotypes and alleles of MPO gene - 463GA and -638CA polymorphic loci in two groups were tested by using allele specific primer PCR ( ASP - PCR ) technique . Results The allele frequencies of MPO gene - 463GA and -638CA in the sample population were polymorphic . The allele frequencies of MPO gene - 463GA polymorphic loci were 0.762 , 0.238 , 0.779 , 0.221 , 0.154 , 0.812 , 0.188 respectively . There was no significant difference between the two groups ( P0.05 ) . Logistic regression analysis showed that the genotype of -463GA and -638CA polymorphic loci had no effect on the risk of vascular dementia ( P0.05 ) . In the " -463GG " subgroup of the vascular dementia group , the prevalence of diabetes mellitus ( P 0.05 ) was higher than that in the " -638CC " type subgroup , whereas in the ' - 463GA + AA " subgroup , there was no significant difference in the incidence of diabetes between the two groups ( P0.05 ) . There was no significant difference in the prevalence of hypertension among the two different genotypes of the -463 locus in the vascular dementia group ( P 0.05 ) . Conclusion The 1MPO gene - 463GA and -638CA polymorphism were not related to the risk of vascular dementia . 2MPO gene - 463GA and -638CA polymorphism , to some extent , affect the risk of diabetes mellitus in patients with vascular dementia .

【學(xué)位授予單位】：新鄉(xiāng)醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R749.13

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相關(guān)期刊論文 前2條

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本文編號(hào)：1408082