AIM To evaluate the effect of oxymatrine（OMT） on hepatocyte apoptosis in rats with lipopolysaccharide（LPS）/D-galactosamine（D-Gal N）-induced acute liver failure（ALF）. METHODS LPS/D-Gal N was used to establish a model of ALF in rats. To evaluate the effect of OMT, we assessed apoptosis by transmission electron microscopy, and the pathological changes in the liver by light microscopy with hematoxylin and eosin staining. An automated biochemical analyzer was used to measure serum alanine aminotransf... 

【文章來(lái)源】：World Journal of Gastroenterology. 2017,23(21)SCI

【文章頁(yè)數(shù)】：11 頁(yè)

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Reagents and antibodies
    Treatment of animals
    Pathological examination of the liver
    Transmission electron microscopy
    Liver function tests
    Tumor necrosis factor-αand interleukin-1βdetection
    Flow cytometry
    Western blotting
    Immunohistochemistry
    Statistical analysis
RESULTS
    General observation of rats
    Transmission electron microscopy
    Effect of OMT on ALT and AST levels
    Effect of OMT on TNF-αand IL-1βexpression
    Apoptosis of hepatocytes
    Effect of OMT on protein expression of the TLR4/PI3K/Akt/GSK-3βsignaling pathway in liver tissue
    Effect of OMT on active caspase-3 and Bax/Bcl-2 in liver tissue
    Effect of OMT on TLR4, active caspase-3, Bax and Bcl-2in liver tissue
DISCUSSION
COMMENTS
    Background
    Innovations and breakthroughs
    Applications
    Peer-review



本文編號(hào)：3168901