重癥急性胰腺炎大鼠血清Apelin、C反應(yīng)蛋白及腫瘤壞死因子-α水平變化及臨床意義
發(fā)布時間：2019-07-05 14:41
【摘要】：目的探討重癥急性胰腺炎(SAP)大鼠血清Apelin、C反應(yīng)蛋白(CRP)及腫瘤壞死因子(TNF)-α水平的變化及臨床意義。方法將36只SD雄性大鼠按照隨機(jī)數(shù)字表法分為SAP組、假性手術(shù)組、對照組,每組12只,SAP組按照大鼠體重1 ml/kg劑量膽總管注入50 g/L牛磺膽酸鈉制備SAP模型,假性手術(shù)組行SAP組相同手術(shù)但不注射藥物,對照組正常飼養(yǎng)。采用酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA)法測定Apelin、CRP及TNF-α水平,光鏡觀察SAP胰腺組織變化并進(jìn)行組織病理評分。結(jié)果 SAP組胰腺組織嚴(yán)重水腫,組織細(xì)胞壞死、具有片狀出血斑并且胰腺出現(xiàn)局部皂化斑,假性手術(shù)組正�；蛘咻p微水腫,無明顯異常,對照組組織正常,建模成功。SAP組血清CRP和TNF-α水平均高于假性手術(shù)組和對照組,Apelin明顯低于假性手術(shù)組和對照組(P0.05)。SAP組胰腺組織病理學(xué)評分高于假性手術(shù)組和對照組(P0.05)。SAP組血清Apelin水平與胰腺組織病理學(xué)評分呈負(fù)相關(guān)(r=-0.876,P0.05),CRP和TNF-α水平與胰腺組織病理學(xué)評分呈正相關(guān)(r=0.698、0.724,P0.05)。結(jié)論血清Apelin、CRP及TNF-α水平變化與SAP發(fā)生、發(fā)展密切相關(guān),可以作為疾病嚴(yán)重程度判斷和治療效果評價的重要指標(biāo)。
[Abstract]:Objective to investigate the changes and clinical significance of serum Apelin,C reactive protein (CRP) and tumor necrosis factor (TNF)-偽 in rats with severe acute pancreatitis (SAP). Methods 36 male SD rats were randomly divided into SAP group (n = 12), control group (n = 12) and control group (n = 12). SAP model was established by injecting 50 g / L sodium taurocholate into common bile duct at a dose of 1 ml/kg in SAP group. SAP group was treated with the same operation but not injected with drugs, and the control group was fed normally. The levels of Apelin,CRP and TNF- 偽 were measured by enzyme-linked immunosorbent assay (ELISA). The changes of pancreatic tissue in SAP were observed by light microscope and the pathological score was evaluated. Results the pancreatic tissue of SAP group was severe edema, tissue cell necrosis, flake bleeding spot and local saponification spot of pancreas. There was no obvious abnormality in false operation group, and the control group was normal, and the model was successful. The levels of serum CRP and TNF- 偽 in SAP group were higher than those in pseudo operation group and control group. Apelin was significantly lower than that of pseudo operation group and control group (P 0.05). SAP group was significantly higher than that of pseudo operation group and control group) (P 0.05). SAP group was negatively correlated with pancreatic pathological score (r 鈮，
本文編號：2510598
[Abstract]:Objective to investigate the changes and clinical significance of serum Apelin,C reactive protein (CRP) and tumor necrosis factor (TNF)-偽 in rats with severe acute pancreatitis (SAP). Methods 36 male SD rats were randomly divided into SAP group (n = 12), control group (n = 12) and control group (n = 12). SAP model was established by injecting 50 g / L sodium taurocholate into common bile duct at a dose of 1 ml/kg in SAP group. SAP group was treated with the same operation but not injected with drugs, and the control group was fed normally. The levels of Apelin,CRP and TNF- 偽 were measured by enzyme-linked immunosorbent assay (ELISA). The changes of pancreatic tissue in SAP were observed by light microscope and the pathological score was evaluated. Results the pancreatic tissue of SAP group was severe edema, tissue cell necrosis, flake bleeding spot and local saponification spot of pancreas. There was no obvious abnormality in false operation group, and the control group was normal, and the model was successful. The levels of serum CRP and TNF- 偽 in SAP group were higher than those in pseudo operation group and control group. Apelin was significantly lower than that of pseudo operation group and control group (P 0.05). SAP group was significantly higher than that of pseudo operation group and control group) (P 0.05). SAP group was negatively correlated with pancreatic pathological score (r 鈮，
本文編號：2510598
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