發(fā)布時(shí)間：2019-04-01 18:55

【摘要】：第一部分人膝關(guān)節(jié)滑膜細(xì)胞的體外培養(yǎng) 目的：獲得人滑膜B型細(xì)胞,進(jìn)行后續(xù)研究。方法：提取滑膜組織,消化,培養(yǎng),傳代,鑒定。結(jié)果：獲得人滑膜B型細(xì)胞。結(jié)論：本方法可以獲得人滑膜B型細(xì)胞,滿足實(shí)驗(yàn)的需求。 第二部分tTG. β-NGF及bFGF對(duì)人膝關(guān)節(jié)滑膜細(xì)胞的作用 目的：研究tTG、βNGF及bFGF對(duì)人膝關(guān)節(jié)滑膜B型細(xì)胞的作用。方法：將第一部分培養(yǎng)好的滑膜細(xì)胞鋪板,加入不同濃度的tTG、β-NGF及bFGF,抽取上清,測(cè)Ⅰ型膠原蛋白的含量,加入MTT,測(cè)細(xì)胞增殖,利用Transwel l測(cè)趨化作用。結(jié)果：tTG在20ng/ml之前,對(duì)滑膜B型細(xì)胞有促進(jìn)增殖、分泌作用,之后,隨著濃度的增加,抑制細(xì)胞增殖和分泌Ⅰ型膠原蛋白,tTG對(duì)滑膜B型細(xì)胞無(wú)趨化作用。β-NGF對(duì)滑膜B型細(xì)胞有抑制增殖和分泌Ⅰ型膠原蛋白作用,β-NGF對(duì)滑膜B型細(xì)胞無(wú)趨化作用。bFGF對(duì)滑膜B型細(xì)胞有促進(jìn)增殖、分泌作用,bFGF對(duì)滑膜B型細(xì)胞無(wú)趨化作用。結(jié)論：在滑膜B型細(xì)胞增殖和分泌Ⅰ型膠原蛋白方面,tTG有雙重作用,β-NGF有抑制作用,bFGF有促進(jìn)作用；在滑膜B型細(xì)胞趨化方面,三種均無(wú)影響。
[Abstract]:Objective: to obtain human synovial type B cells for follow-up study. Methods: synovial tissue was extracted, digested, cultured, subcultured and identified. Results: human synovial type B cells were obtained. Conclusion: the human synovial type B cells can be obtained by this method, which can meet the needs of the experiment. Part two tTG. Effects of 尾-NGF and bFGF on human synovial cells objective: to study the effects of tTG, 尾 NGF and bFGF on human synovial type B cells. Methods: the synovial cells were cultured in the first part. Different concentrations of tTG, 尾-NGF and bFGF, were added to extract supernatant. The content of type 鈪，

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