大鼠肺缺血再灌注組織中microRNA-451與蛋白激酶B的表達(dá)變化
發(fā)布時(shí)間：2019-02-14 10:11
【摘要】：目的：探討不同時(shí)相大鼠肺缺血再灌注模型中microRNA-451及蛋白激酶B(p-AKT)的表達(dá)情況及二者之間的相關(guān)性。 方法：將Wistar大鼠隨機(jī)分為五組,分別為對照組、缺血1h組、缺血1h后再灌注組1h、再灌注3h及再灌注6h組,每組10只,參照EPPINGER法建立大鼠肺缺血模型(分別于缺血1h、缺血1h后再灌注組1h、再灌注3h及再灌注6h取材),采用實(shí)時(shí)熒光定量PCR(RT-qPCR)檢測microRNA-451及蛋白免疫印跡法(Western blot)檢測p-AKT蛋白的表達(dá)情況。 結(jié)果:RT-qPCR檢測結(jié)果顯示microRNA-451在肺缺血再灌注過程中呈現(xiàn)先升高后降低的趨勢,其中再灌注3h時(shí)達(dá)到最高點(diǎn),差異有統(tǒng)計(jì)學(xué)意義(F=17244.32p0.05);Western blot檢測法顯示p-AKT蛋白呈先下降后升高的趨勢,對照組及再灌注6h時(shí)呈高表達(dá),再灌注3h時(shí)表達(dá)量最低,差異有顯著性(F=136.02p0.05)。 結(jié)論：肺缺血再灌注過程中,microR-451與p-AKT的表達(dá)變化呈現(xiàn)一定的相關(guān)性,結(jié)合相關(guān)文獻(xiàn),可推測microR-451可能通過調(diào)節(jié)AKT蛋白通路促進(jìn)細(xì)胞凋亡。
[Abstract]:Aim: to investigate the expression of microRNA-451 and protein kinase B (p-AKT) in lung ischemia-reperfusion model of rats and the correlation between them. Methods: Wistar rats were randomly divided into five groups: control group, 1 hour ischemia group, 1 h reperfusion group, 3 h reperfusion group and 6 h reperfusion group, 10 rats in each group. Lung ischemia models were established by EPPINGER method. The expression of p-AKT protein was detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot (Western blot). Results: the results of RT-qPCR showed that microRNA-451 increased at first and then decreased during lung ischemia-reperfusion, and reached the highest point at 3 h after reperfusion, and the difference was statistically significant (F=17244.32p0.05). Western blot assay showed that the expression of p-AKT protein decreased first and then increased. The expression of p-AKT protein in the control group was higher than that in the control group at 6 h and the lowest at 3 h after reperfusion. The difference was significant (F=136.02p0.05). Conclusion: the expression of microR-451 and p-AKT is correlated during lung ischemia-reperfusion. It can be speculated that microR-451 can promote apoptosis by regulating AKT protein pathway.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R655.3
本文編號：2422113
[Abstract]:Aim: to investigate the expression of microRNA-451 and protein kinase B (p-AKT) in lung ischemia-reperfusion model of rats and the correlation between them. Methods: Wistar rats were randomly divided into five groups: control group, 1 hour ischemia group, 1 h reperfusion group, 3 h reperfusion group and 6 h reperfusion group, 10 rats in each group. Lung ischemia models were established by EPPINGER method. The expression of p-AKT protein was detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot (Western blot). Results: the results of RT-qPCR showed that microRNA-451 increased at first and then decreased during lung ischemia-reperfusion, and reached the highest point at 3 h after reperfusion, and the difference was statistically significant (F=17244.32p0.05). Western blot assay showed that the expression of p-AKT protein decreased first and then increased. The expression of p-AKT protein in the control group was higher than that in the control group at 6 h and the lowest at 3 h after reperfusion. The difference was significant (F=136.02p0.05). Conclusion: the expression of microR-451 and p-AKT is correlated during lung ischemia-reperfusion. It can be speculated that microR-451 can promote apoptosis by regulating AKT protein pathway.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R655.3
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 周曉東,于皆平,于紅剛,羅和生,呂農(nóng)華,朱萱;蛋白激酶B在胃癌中的表達(dá)及其生物學(xué)意義[J];中華消化雜志;2005年07期
，本文編號：2422113
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