【摘要】：目的:觀察低功率氦氖激光照射后燙傷小鼠創(chuàng)面的變化及其對小鼠創(chuàng)面炎癥介導(dǎo)因子VEGF、Caspase-3基因表達(dá)的影響。方法:60只燙傷小鼠模型隨機(jī)分為激光照射組與對照組,其中激光照射組行低強(qiáng)度氦氖激光照射燙傷創(chuàng)面,能量密度15J/cm2,對照組采用假照射,能量密度0J/cm2,每天一次,每次15min,連續(xù)14d。分別在燙傷后0、1、3、7、14d記錄創(chuàng)面面積,計(jì)算創(chuàng)面愈合率,并取全層創(chuàng)面組織標(biāo)本,采用RT-PCR法檢測VEGF、Caspase-3的基因表達(dá)。結(jié)果:激光照射組小鼠創(chuàng)面愈合率明顯高于對照組(P0.05)。與對照組相比,激光照射組VEGF基因表達(dá)在燙傷后1d時(shí)降低,3d時(shí)開始增加(P0.05),7d時(shí)達(dá)到高峰(P0.01),隨后降低,在14d時(shí),VEGF基因表達(dá)已低于對照組(P0.05)。激光組1d時(shí)Caspase-3基因表達(dá)減少(P0.05),3d時(shí)Caspase-3基因表達(dá)持續(xù)降低(P0.01),7d時(shí)開始增高(P0.05)。結(jié)論:低功率氦氖激光照射可以加速燙傷創(chuàng)面愈合,可能與其調(diào)節(jié)VEGF和Caspase-3因子表達(dá)相關(guān)。
[Abstract]:Aim: to observe the effect of low power He-Ne laser irradiation on burn wound and the expression of inflammatory mediating factor (VEGF,Caspase-3) in burn mice. Methods: sixty scalded mice were randomly divided into laser irradiation group and control group. The laser irradiation group received low intensity He-Ne laser irradiation on the burn wound, the energy density was 15J / cm ~ 2, while the control group was treated with false irradiation, the energy density was 0 J / cm ~ 2, once a day. Each time 15 minutes, continuous 14 days. The wound area was recorded and the rate of wound healing was calculated on the 14th day after scald, and the VEGF,Caspase-3 gene expression was detected by RT-PCR method. Results: the wound healing rate in laser irradiation group was significantly higher than that in control group (P 0.05). Compared with the control group, the expression of VEGF gene in laser irradiation group decreased at 1 day after scalding, began to increase at 3 days (P0.05), reached its peak at 7 days (P0.01), and then decreased. At 14 days, the expression of VEGF gene was lower than that in control group (P0.05). In the laser group, the expression of Caspase-3 gene decreased at day 1 (P0.05), the expression of Caspase-3 gene continued to decrease at day 3 (P0.01), and the expression began to increase at day 7 (P0.05). Conclusion: low power He-Ne laser irradiation can accelerate wound healing, which may be related to the regulation of VEGF and Caspase-3 expression.
【作者單位】： 第四軍醫(yī)大學(xué)附屬西京醫(yī)院康復(fù)理療科;青島第一療養(yǎng)院;
【基金】：陜西省自然科學(xué)基礎(chǔ)研究計(jì)劃項(xiàng)目(210JM4019) 應(yīng)用基礎(chǔ)研究項(xiàng)目(BWS11J003)
【分類號】：R644

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