【摘要】：目的：把煙霧吸入性損傷大鼠做為本次試驗(yàn)的研究對(duì)象,對(duì)其腹腔注射氮硝基左旋精氨酸(Nω-Nitro-L-Arginine, L-NNA),觀察不同劑量、不同作用時(shí)長(zhǎng)L-NNA的治療效果,明確并評(píng)價(jià)L-NNA對(duì)煙霧吸入性損傷大鼠的肺保護(hù)作用。方法：將60只大鼠隨機(jī)分為4組,分別為空白組、致傷組、低劑量組和高劑量組,其中空白組6只大鼠不致傷,其余3組分為6h、24h、48h三個(gè)時(shí)相點(diǎn),各組各時(shí)相點(diǎn)6只大鼠,在特制裝置中用煙霧致傷�？瞻捉M不予任何處理,致傷組、低劑量及高劑量組于傷后立即各自腹腔注射等容量的生理鹽水、濃度分別為1Omg/kg的L-NNA和20mg/kg的L-NNA。每只大鼠股動(dòng)脈取血5ml,離心后檢測(cè)血清白細(xì)胞介素-6(Interleukin-6, IL-6)、白細(xì)胞介素-10(Interleukin-10, IL-10)、腫瘤壞死因子-α(Tumomecrosis factor-α, TNF-α)含量,活殺后取部分左側(cè)肺組織勻漿后檢測(cè)丙二醛(Malondialdehyde, MDA)、髓過(guò)氧化物酶(Myeloperoxidase, MPO)及超氧化物歧化酶(Superoxide dismutase, SOD)含量,取部分右肺經(jīng)4%甲醛溶液固定后做病理HE染色切片光鏡觀察。 結(jié)果：低劑量組24h、48h和高劑量組6h、24h、48h的TNF-α、IL-6含量與同時(shí)相點(diǎn)致傷組相比明顯下降(P0.01),低劑量組6h和高劑量組6h、24h、48h的MDA、MPO含量與同時(shí)相點(diǎn)致傷組相比明顯降低(P0.01),高劑量組6h、24h、48h的IL-10、SOD含量與同時(shí)相點(diǎn)致傷組相比明顯升高(P0.01),高劑量組6h、24h、48h的IL-6、TNF-α、MDA、MPO含量比同時(shí)相點(diǎn)低劑量組明顯降低(P0.01), IL-10、SOD含量相比同時(shí)相點(diǎn)低劑量組明顯升高(P0.01)。低劑量組大鼠6h、24h、48h和高劑量組大鼠6h、24h、48h肺系數(shù)與同時(shí)相點(diǎn)致傷組大鼠相比有顯著降低(P0.01),與低劑量組同時(shí)相點(diǎn)大鼠相比,高劑量組大鼠肺系數(shù)均有明顯降低(P0.01),病理切片顯示低劑量組6h和高劑量組6h大鼠相較致傷組6h肺組織肺水腫減輕,中性粒細(xì)胞、淋巴細(xì)胞等炎細(xì)胞的浸潤(rùn)減少,肺泡萎陷程度降低,其中高劑量組比低劑量組肺間質(zhì)和肺泡內(nèi)滲出減少。 結(jié)論：氮硝基左旋精氨酸可減輕大鼠吸入性損傷后肺組織中炎癥反應(yīng)及過(guò)氧化反應(yīng),從而減輕肺損傷的程度,而高劑量組比低劑量組的肺保護(hù)作用更好。
[Abstract]:Objective: to observe the therapeutic effect of long L-NNA in rats with smoke inhalation injury by intraperitoneal injection of N 蠅 -Nitro-L-Arginine (L-NNA). To determine and evaluate the protective effect of L-NNA on lung injury induced by smoke inhalation in rats. Methods: sixty rats were randomly divided into 4 groups: blank group, injury group, low dose group and high dose group. Among them, 6 rats in blank group were not injured, and the other three groups were divided into 3 groups: 6 rats in each group, and 6 rats in each group. To cause injuries with smoke in a special device. No treatment was given in the blank group. The injured group, the low dose group and the high dose group were intraperitoneally injected with normal saline of equal volume immediately after injury. The concentrations of L-NNA of 1Omg/kg and L-NNA of 20mg/kg were respectively. Serum interleukin-6 (Interleukin-6, IL-6), interleukin-10 (Interleukin-10, IL-10) and tumor necrosis factor- 偽 (TNF- 偽) were measured after centrifugation. The contents of malondialdehyde (Malondialdehyde, MDA),) myeloperoxidase (Myeloperoxidase, MPO) and superoxide dismutase (Superoxide dismutase, SOD) were detected in the homogenate of some left lung tissues. The pathological sections of the right lung were fixed with 4% formaldehyde solution and observed under light microscope. Results: the content of TNF- 偽 IL-6 in low dose group (24 h) and high dose group (6 h / 24 h) was significantly lower than that in simultaneous point group (P0.01). The content of MDA,MPO in low dose group (6 h) and high dose group (6 h) was significantly lower than that in simultaneous phase group (P 0.01). The IL-10,SOD in high dose group was significantly lower than that in high dose group (6 h, 24 h and 48 h). The content of IL-6,TNF- 偽 -MDA-MPO in the high dose group was significantly lower than that in the low dose group (P0.01), and the content of IL-10,SOD in the high dose group was significantly higher than that in the low-dose group at the same phase point (P0.01). The content of MDA-MPO in the high-dose group was significantly higher than that in the low-dose group at the same time (P0.01). The lung coefficients of the rats in the low dose group and the high dose group were significantly lower than those in the simultaneous phase point group (P0.01), and compared with those in the low dose group (P0.01). The lung coefficients of the rats in the high dose group were significantly decreased (P0.01). Pathological sections showed that lung edema, infiltration of neutrophils, lymphocytes and other inflammatory cells in the lung tissue of the rats in the low dose group at 6 h and in the high dose group at 6 h were less than those in the injured group at 6 h after injury (P0.01), and the infiltration of inflammatory cells such as neutrophils and lymphocytes decreased. The degree of alveolar collapse was decreased, and the interstitial and alveolar effusion in high dose group was lower than that in low dose group. Conclusion: nitro-L-arginine can reduce the inflammatory reaction and peroxidation in lung tissue after inhalation injury in rats, thus reducing the severity of lung injury. The protective effect of high dose group is better than that of low dose group.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R644

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