發(fā)布時(shí)間：2018-08-13 11:07

【摘要】：目的比較研究不同肩袖損傷類型以及采用不同方法修復(fù)后的肩袖愈合差異,探討能準(zhǔn)確模擬臨床肩袖損傷修復(fù)術(shù)后恢復(fù)進(jìn)程的動(dòng)物模型。方法取12只成年雄性比格犬,體質(zhì)量10~15 kg,根據(jù)處理方法不同隨機(jī)分為3組(n=4),分別制備急性肩袖損傷+Mason-Allen縫合修復(fù)(A組)、巨大肩袖損傷+Mason-Allen縫合修復(fù)(B組)及巨大肩袖損傷+Mason-Allen縫合并自體半腱肌擴(kuò)張部修復(fù)(C組)動(dòng)物模型;3組修復(fù)后均外固定架制動(dòng)。術(shù)后觀察各組動(dòng)物一般情況;于術(shù)后6周取材大體觀察岡下肌肌腱末端愈合情況,生物力學(xué)測(cè)試極限負(fù)荷,組織學(xué)觀察肌腱細(xì)胞及纖維改變,比較肩袖修復(fù)程度。結(jié)果術(shù)后各組動(dòng)物均存活至實(shí)驗(yàn)完成,切口均愈合良好,無感染發(fā)生。大體觀察示,A組岡下肌肌腱末端瘢痕組織明顯多于正常肌腱組織;B組岡下肌肌腱末端未見明顯肌腱組織;C組岡下肌肌腱雖然部分覆蓋瘢痕組織,但仍可觀察到肌腱及其大致走向。A、B、C組極限負(fù)荷分別為(223.75±24.28)、(159.25±34.87)、(233.25±14.24)N,B組顯著低于A、C組(P0.05),A、C組間比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。組織學(xué)觀察示,A組肌腱纖維排列大致正常;B組肌腱纖維排列較紊亂,并且肌腱細(xì)胞明顯少于A組;C組肌腱纖維排列整齊,且肌腱細(xì)胞多于B組。結(jié)論 Mason-Allen縫合聯(lián)合自體半腱肌擴(kuò)張部修復(fù)犬巨大肩袖損傷能獲良好修復(fù)效果;制動(dòng)模型可較好模擬臨床肩袖損傷愈合過程,可作為相關(guān)研究的理想動(dòng)物模型。
[Abstract]:Objective to compare the different types of rotator cuff injury and the difference of rotator cuff healing after repair with different methods, and to explore an animal model which can accurately simulate the recovery process of clinical rotator cuff injury repair. Methods 12 adult male Beagle dogs were selected. The patients were randomly divided into 3 groups according to different treatment methods: Mason-Allen suture repair for acute rotator cuff injury (group A), Mason-Allen suture repair for giant rotator cuff injury (group B) and Mason-Allen suture with autologous semitendinosus for giant rotator cuff injury. Muscle dilatation repair (group C) animal model group 3 after repair of external fixator brake. The general condition of each group was observed 6 weeks after operation, the healing of tendon end of inferior spinal muscle was observed, the limit load of biomechanics was measured, the changes of tendon cells and fibers were observed by histology, and the degree of rotator cuff repair was compared. Results all the animals survived to the end of the experiment, the incision healed well and no infection occurred. Gross observation showed that the scar tissue at the end of subspinous muscle in group A was significantly more than that in group B, and no obvious tendon tissue was found at the end of tendon in group C although the tendon in group C was partly covered with scar tissue. However, the ultimate load of tendon and its approximate direction in group C was (223.75 鹵24.28), () 159.25 鹵34.87), (233.25 鹵14.24), (, respectively, which was significantly lower than that in group A (P 0.05). There was no significant difference between group A and C (P0.05). Histological observation showed that the arrangement of tendon fibers in group A was generally normal. The arrangement of tendon fibers in group B was disordered, and the number of tendon cells in group A was significantly less than that in group A and C, and the number of tendon cells in group B was more than that in group B. Conclusion Mason-Allen suture combined with autologous semitendinosus expansion can be used to repair the giant rotator cuff injury in dogs, and the immobilization model can be used to simulate the healing process of clinical rotator cuff injury, and it can be used as an ideal animal model for related research.
【作者單位】： 南方醫(yī)科大學(xué)南京臨床醫(yī)學(xué)院附屬南京軍區(qū)南京總醫(yī)院骨科;
【基金】：江蘇省臨床醫(yī)學(xué)科技專項(xiàng)資助項(xiàng)目(BL2012002)~~
【分類號(hào)】：R686;R-332

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1 李奉龍;姜春巖;魯誼;黎廣平;盧耀甲;;兔肩袖損傷模型的建立及初步組織學(xué)研究[J];中國(guó)組織工程研究;2012年20期

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本文編號(hào)：2180791