【摘要】：目的:觀察高膽紅素血癥(hyperbilirubinemia)腦損傷新生大鼠血清S100B蛋白水平及依達(dá)拉奉(edaravone,MCI-186)干預(yù)后其濃度的變化。探討依達(dá)拉奉對(duì)高膽紅素血癥腦損傷的作用,為臨床上依達(dá)拉奉防治膽紅素腦病提供一定的實(shí)驗(yàn)依據(jù)。方法:將96只7日齡SD(Sprague-Dawley,SD)大鼠隨機(jī)分為3組:正常對(duì)照組、高膽紅素血癥組、依達(dá)拉奉干預(yù)組,即A、B、C三組,每組32只大鼠。A、B、C組再依據(jù)處死時(shí)間點(diǎn)分為4個(gè)亞組:24h組、48h組、72h組和96h組,每組8只大鼠。采取腹腔注射的方法給B、C組大鼠按100mg/kg的劑量注入1次膽紅素,用同樣方法給A組大鼠按同樣劑量注入1次生理鹽水,造模后C組大鼠立即接受依達(dá)拉奉干預(yù),使用同樣方法注入2mg/kg/d,連續(xù)4天,最后在各不同處死時(shí)間點(diǎn)盡快將各組大鼠的血清及腦組織取出。用HE(Hematoxylin and eosin)染色方法觀察大鼠腦組織的病理學(xué)改變,用Elisa法測(cè)血清中S100B蛋白的濃度,然后記錄實(shí)驗(yàn)結(jié)果、計(jì)算相關(guān)數(shù)據(jù)并對(duì)其進(jìn)行統(tǒng)計(jì)學(xué)分析,并得出結(jié)論。結(jié)果:(1)造模后,明顯的神經(jīng)行為異常均出現(xiàn)在B、C組大鼠,各不同時(shí)間點(diǎn)C組大鼠癥狀較B組的明顯改善,而A組大鼠行為無(wú)明顯異常。(2)造模24h后,B、C組大鼠腦組織出現(xiàn)水平不一的水腫、變性及炎性浸潤(rùn)等,隨著時(shí)間延長(zhǎng)其程度加重。各處死時(shí)間點(diǎn)C組大鼠腦組織改變程度較B組的減輕,A組大鼠腦組織結(jié)構(gòu)基本正常。(3)B組大鼠血清S100B蛋白的濃度顯著高于A、C兩組,且和時(shí)間呈正相關(guān)性,差異存在統(tǒng)計(jì)學(xué)意義(P0.05);C組大鼠血清S100B蛋白的濃度顯著低于B組,高于A組,差異存在統(tǒng)計(jì)學(xué)意義(P0.05);A組大鼠血清S100B蛋白水平較低,各處死時(shí)間點(diǎn)組內(nèi)相比,差異沒(méi)有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:(1)高膽紅素血癥時(shí),大鼠血清S100B蛋白水平明顯升高,且和時(shí)間呈正相關(guān)性;經(jīng)藥物干預(yù)后,血清S100B蛋白水平明顯降低。(2)高膽紅素血癥時(shí),24h后大鼠腦組織出現(xiàn)水腫、變性及炎性浸潤(rùn)等改變,且隨著時(shí)間延長(zhǎng),其程度有所加重;經(jīng)依達(dá)拉奉干預(yù)后,不同時(shí)間點(diǎn)大鼠腦組織的水腫、變性及炎性浸潤(rùn)等可被不同程度減輕.(3)表明依達(dá)拉奉可減輕過(guò)量膽紅素對(duì)中樞神經(jīng)系統(tǒng)的損傷,起到保護(hù)神經(jīng)元的作用。
[Abstract]:Aim: to observe the changes of serum S100B protein and the concentration of Edaravone MCI-186 in neonatal rats with hyperbilirubinemia (hyperbilirubinemia) brain injury. To investigate the effect of Edaravone on cerebral injury of hyperbilirubinemia and to provide experimental evidence for the prevention and treatment of bilirubin encephalopathy by Edaravone. Methods: Ninety-six 7-day-old SD rats were randomly divided into three groups: normal control group, hyperbilirubinemia group, Edaravone intervention group, Acarb Bon C group. The 32 rats in each group were divided into 4 subgroups according to the time point of death. They were divided into 4 subgroups: 24 h group, 48 h group, 72 h group and 96 h group, 8 rats in each group. Rats in group C were injected bilirubin once according to the dose of 100mg/kg, and rats in group A were injected with normal saline at the same dose by intraperitoneal injection. The rats in group C were treated with Edaravone immediately after the establishment of the model. The same method was used to inject 2 mg / kg / d for 4 consecutive days. Finally, the serum and brain tissues of each group were removed as soon as possible at different time points. The pathological changes of brain tissue in rats were observed by HE (Hematoxylin and eosin) staining. The concentration of S100B protein in serum was measured by Elisa method. The experimental results were recorded, the relevant data were calculated and statistically analyzed, and the conclusion was reached. Results: (1) after modeling, the obvious neurobehavioral abnormalities were found in rats of group B, and the symptoms of group C were significantly improved compared with those of group B at different time points. But there was no obvious abnormal behavior in group A. (2) after 24 hours of modeling, there were edema, degeneration and inflammatory infiltration in brain tissue of rats in group B and C, which increased with time. The changes of brain tissue in group C were normal compared with those in group B. (3) the concentration of serum S100B protein in group B was significantly higher than that in group A and C, and had a positive correlation with time. The difference was statistically significant (P0.05) the concentration of serum S100B protein in group C was significantly lower than that in group B and higher than that in group A (P0.05). The level of S100B protein in group A was lower than that in group A (P0.05). Conclusion: (1) at hyperbilirubinemia, the serum S100B protein level was significantly increased and positively correlated with the time, and the serum S100B protein level was significantly decreased after drug intervention. (2) there was edema in the brain tissue of rats with hyperbilirubinemia at 24 h after hyperbilirubinemia. The degree of degeneration and inflammatory infiltration was increased with time. After Edaravone intervention, edema of brain tissue was observed in rats at different time points. Denaturation and inflammatory infiltration can be alleviated in varying degrees. (3) Edaravone can reduce the damage of excessive bilirubin to the central nervous system and protect neurons.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R651.15

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