【摘要】：目的觀察骨髓間質(zhì)干細(xì)胞(BMSCs)對(duì)犬急性缺血腦組織的保護(hù)作用,并探討其可能的機(jī)制。方法將24只成年雜交犬隨機(jī)分為治療組及對(duì)照組,DSA引導(dǎo)下行自體血栓栓塞大腦中動(dòng)脈閉塞缺血模型制作,并抽取骨髓提取BMSCs,傳代并給予4’,6-二脒基-2-苯基吲哚(DAPI)標(biāo)記;1周后開顱行多點(diǎn)腦內(nèi)注射BMSCs移植;移植后1周行腦DWI序列掃描,計(jì)算梗死灶體積;4周后將犬處死,每組隨機(jī)選擇6只動(dòng)物取腦標(biāo)本行TTC染色測(cè)定梗死灶體積;另外6只動(dòng)物進(jìn)行HE染色、VG染色、TUNEL染色評(píng)價(jià)腦損傷情況;免疫熒光染色了解腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)、堿性成纖維生長(zhǎng)因子(b FGF)、胰島素樣生長(zhǎng)因子1(IGF-1)和血管內(nèi)皮生長(zhǎng)因子(VEGF)的表達(dá)情況。結(jié)果治療組梗死灶內(nèi)廣泛存在DAPI陽(yáng)性細(xì)胞。治療組的梗死灶體積明顯小于對(duì)照組,P0.01。治療組梗死灶范圍、梗死灶內(nèi)細(xì)胞壞死、膠質(zhì)增生和膠質(zhì)瘢痕均較對(duì)照組減輕。治療組凋亡細(xì)胞明顯少于對(duì)照組,P0.05。治療組細(xì)胞因子BDNF、BFGF、IGF-1和VEGF表達(dá)陽(yáng)性的細(xì)胞均顯著多于對(duì)照組。結(jié)論腦梗死后給予BMSCs移植,BMSCs能存活并自行向梗死灶遷移,并發(fā)揮腦保護(hù)作用,其機(jī)制可能和分泌多種神經(jīng)營(yíng)養(yǎng)因子有關(guān)。
[Abstract]:Objective to investigate the protective effect of bone marrow mesenchymal stem cell (BMSCs) on acute ischemic brain tissue in dogs and its possible mechanism. Methods Twenty-four adult hybrid dogs were randomly divided into treatment group and control group. BMSCs were extracted from bone marrow, then subcultured and labeled with 4-diamidine-2-phenylindole-indole (DAPI) for 1 week, then BMSCs was injected into the brain after craniotomy, and brain DWI sequence scanning was performed 1 week after transplantation, and the dogs were killed after 4 weeks of infarct volume calculation. The infarct volume was determined by TTC staining in 6 animals in each group, and the brain injury was evaluated by HE staining with VG staining and Tunel staining. The expression of (BDNF), basic fibroblast growth factor (b FGF),) insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) were detected by immunofluorescence staining. Results DAPI positive cells were found in infarct focus of treatment group. The infarct volume in the treatment group was significantly smaller than that in the control group (P 0.01). The infarct area, cell necrosis, glial hyperplasia and glial scar in the treatment group were less than those in the control group. The apoptotic cells in the treatment group were significantly lower than those in the control group (P 0.05). The positive expression of BDNF BFGFF-1 and VEGF in the treatment group was significantly higher than that in the control group. Conclusion BMSCs can survive and migrate to the infarct area after cerebral infarction, and exert the protective effect of brain. The mechanism may be related to the secretion of many neurotrophic factors.
【作者單位】： 江西省宜春市人民醫(yī)院神經(jīng)內(nèi)科;
【基金】：江西省科技計(jì)劃項(xiàng)目(20112BBG70085)
【分類號(hào)】：R-332;R743.33

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