網膜素-1與急性腦梗死發(fā)病相關性研究
發(fā)布時間：2018-07-25 08:13
【摘要】：目的 通過檢測急性腦梗死患者與健康對照組的血清網膜素-1表達水平,以及急性腦梗死不同分組及其不同亞型中血清網膜素-1的表達水平,探討網膜素-1在急性腦梗死發(fā)病中所起的作用,并進一步探討網膜素-1參與腦梗死發(fā)病的可能機制。 方法 根據納入標準和排除標準,連續(xù)性收集180例門診及住院的急性腦梗死患者,另外取健康體檢者180例作為對照。根據頸動脈彩超檢查將腦梗死患者分為無動脈粥樣硬化組、穩(wěn)定型斑塊組及不穩(wěn)定型斑塊組,同時采用牛津郡社區(qū)卒中項目(Oxfordshire Community Stroke Project,OCSP)將腦梗死患者分為四個不同的臨床類型：1、全前循環(huán)腦梗死；2、部分前循環(huán)腦梗死3、后循環(huán)腦梗死4、腔隙性腦梗死。采用雙抗體夾心酶聯免疫吸附法(ELISA)測定標本中血清網膜素-1水平.檢測急性腦梗死患者及健康體檢者空腹血清網膜素-1水平。統計學分析采用SPSS19.0軟件,所有實驗數據以x±s表示,應用雙側t檢驗比較人網膜素-1在急性腦梗死患者以及正常對照人群中的差異性表達。應用單因素方差分析來比較人網膜素-1在無動脈粥樣硬化組、穩(wěn)定型斑塊組和不穩(wěn)定型斑塊組中的差異性表達；應用單因素方差分析,比較人網膜素-1在不同亞型急性腦梗死患者中的差異性表達,p0.05為差異具有統計學意義。 結果 血清網膜素-1水平在急性腦梗死患者中顯著低于健康對照人群(p0.05)；在穩(wěn)定型斑塊組及不穩(wěn)定型斑塊組患者中顯著低于無動脈粥樣硬化組(p0.05,p0.05),而在穩(wěn)定型斑塊組及不穩(wěn)定型斑塊組之間無顯著性差異(p0.05)；在全前循環(huán)梗死型、部分前循環(huán)梗死型以及后循環(huán)梗死型中顯著低于腔隙性梗死型(p0.05,p0.05,p0.05),而在全前循環(huán)梗死型、部分前循環(huán)梗死型以及后循環(huán)梗死型之間無顯著性差異(p0.05)。 結論 血清網膜素-1水平在急性腦梗死患者中顯著低于健康對照組人群；在穩(wěn)定型斑塊組及不穩(wěn)定型斑塊組患者中顯著低于無動脈粥樣硬化組,而在穩(wěn)定型板塊組及不穩(wěn)定型斑塊組之間無顯著性差異；在全前循環(huán)梗死型、部分前循環(huán)梗死型以及后循環(huán)梗死型中顯著低于腔隙性梗死型,而在全前循環(huán)梗死型、部分前循環(huán)梗死型以及后循環(huán)梗死型之間無顯著性差異。由于血清網膜素-1在急性腦梗死患者及其不同亞型中存在的差異性表達,以及與健康對照人群的顯著性差異,可以推測血清網膜素-1將來可能成為一種新的急性腦梗死診斷、嚴重程度及預后判斷的指標。
[Abstract]:Objective to detect the level of serum omentin-1 in patients with acute cerebral infarction and healthy controls, and to detect the expression of omentin-1 in different subgroups and subtypes of acute cerebral infarction. To explore the role of omentin--1 in the pathogenesis of acute cerebral infarction and to further explore the possible mechanism of retinin--1 involved in the pathogenesis of cerebral infarction. Methods 180 consecutive patients with acute cerebral infarction were collected according to the inclusion criteria and exclusion criteria, and 180 healthy persons were taken as control. Patients with cerebral infarction were divided into three groups according to carotid color Doppler ultrasonography: no atherosclerosis group, stable plaque group and unstable plaque group. At the same time, the patients with cerebral infarction were divided into four different clinical types: 1: 1, 2 cases of total anterior circulation infarction, 3 cases of partial anterior circulation cerebral infarction, 4 cases of posterior circulation cerebral infarction and 4 lacunar cerebral infarction using Oxfordshire Community Stroke Project (Oxfordshire Community Stroke Project OCSP). The level of omentin-1 in serum was determined by double antibody sandwich enzyme linked immunosorbent assay (ELISA). Fasting serum omentin-1 levels were measured in patients with acute cerebral infarction and healthy controls. Statistical analysis was performed with SPSS19.0 software, and all experimental data were expressed as x 鹵s. The expression of human omentin-1 in patients with acute cerebral infarction and normal controls was compared by using bilateral t test. Univariate analysis of variance was used to compare the differential expression of human omentin-1 in atherosclerotic, stable and unstable plaque groups. The difference of expression of human omentin-1 in different subtypes of acute cerebral infarction was statistically significant. Results the serum level of omentin-1 in patients with acute cerebral infarction was significantly lower than that in healthy controls (p0.05). In stable plaque group and unstable plaque group, it was significantly lower than that in non-atherosclerosis group (p0.05), but there was no significant difference between stable plaque group and unstable plaque group (p0.05), and in total anterior circulation infarction type, there was no significant difference between stable plaque group and unstable plaque group (p0.05). The incidence of partial anterior circulation infarction and posterior circulation infarction was significantly lower than that of lacunar infarction (p0.05), but there was no significant difference among total anterior circulation infarction, partial anterior circulation infarction and posterior circulation infarction (p0.05). Conclusion the serum level of omentin-1 in patients with acute cerebral infarction is significantly lower than that in healthy controls, and that in stable plaque and unstable plaque is significantly lower than that in non-atherosclerosis. However, there was no significant difference between the stable plate group and unstable plaque group, and in the total anterior circulation infarction type, partial anterior circulation infarction type and posterior circulation infarction type were significantly lower than that in the lacunar infarction type, but in the total anterior circulation infarction type. There was no significant difference between partial anterior circulation infarction type and posterior circulation infarction type. Because of the differential expression of serum omentin-1 in patients with acute cerebral infarction and its subtypes, and the significant difference between serum omentin-1 and healthy controls, it can be speculated that serum omentin--1 may become a new diagnosis of acute cerebral infarction in the future. Severity and prognostic criteria.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R743.33
本文編號：2143189
[Abstract]:Objective to detect the level of serum omentin-1 in patients with acute cerebral infarction and healthy controls, and to detect the expression of omentin-1 in different subgroups and subtypes of acute cerebral infarction. To explore the role of omentin--1 in the pathogenesis of acute cerebral infarction and to further explore the possible mechanism of retinin--1 involved in the pathogenesis of cerebral infarction. Methods 180 consecutive patients with acute cerebral infarction were collected according to the inclusion criteria and exclusion criteria, and 180 healthy persons were taken as control. Patients with cerebral infarction were divided into three groups according to carotid color Doppler ultrasonography: no atherosclerosis group, stable plaque group and unstable plaque group. At the same time, the patients with cerebral infarction were divided into four different clinical types: 1: 1, 2 cases of total anterior circulation infarction, 3 cases of partial anterior circulation cerebral infarction, 4 cases of posterior circulation cerebral infarction and 4 lacunar cerebral infarction using Oxfordshire Community Stroke Project (Oxfordshire Community Stroke Project OCSP). The level of omentin-1 in serum was determined by double antibody sandwich enzyme linked immunosorbent assay (ELISA). Fasting serum omentin-1 levels were measured in patients with acute cerebral infarction and healthy controls. Statistical analysis was performed with SPSS19.0 software, and all experimental data were expressed as x 鹵s. The expression of human omentin-1 in patients with acute cerebral infarction and normal controls was compared by using bilateral t test. Univariate analysis of variance was used to compare the differential expression of human omentin-1 in atherosclerotic, stable and unstable plaque groups. The difference of expression of human omentin-1 in different subtypes of acute cerebral infarction was statistically significant. Results the serum level of omentin-1 in patients with acute cerebral infarction was significantly lower than that in healthy controls (p0.05). In stable plaque group and unstable plaque group, it was significantly lower than that in non-atherosclerosis group (p0.05), but there was no significant difference between stable plaque group and unstable plaque group (p0.05), and in total anterior circulation infarction type, there was no significant difference between stable plaque group and unstable plaque group (p0.05). The incidence of partial anterior circulation infarction and posterior circulation infarction was significantly lower than that of lacunar infarction (p0.05), but there was no significant difference among total anterior circulation infarction, partial anterior circulation infarction and posterior circulation infarction (p0.05). Conclusion the serum level of omentin-1 in patients with acute cerebral infarction is significantly lower than that in healthy controls, and that in stable plaque and unstable plaque is significantly lower than that in non-atherosclerosis. However, there was no significant difference between the stable plate group and unstable plaque group, and in the total anterior circulation infarction type, partial anterior circulation infarction type and posterior circulation infarction type were significantly lower than that in the lacunar infarction type, but in the total anterior circulation infarction type. There was no significant difference between partial anterior circulation infarction type and posterior circulation infarction type. Because of the differential expression of serum omentin-1 in patients with acute cerebral infarction and its subtypes, and the significant difference between serum omentin-1 and healthy controls, it can be speculated that serum omentin--1 may become a new diagnosis of acute cerebral infarction in the future. Severity and prognostic criteria.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R743.33
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