【摘要】：目的研究抗氧化劑對熱打擊過程中人臍動脈血管平滑肌細(xì)胞(HUASMCs)收縮反應(yīng)的影響。方法將HUASMCs分為對照組、熱打擊組(HS組)和熱打擊前超氧化物歧化酶(SOD)預(yù)處理組(SOD+HS組),每組分別用低濃度NE(0.05mg/L)及常規(guī)濃度NE(1.0mg/L)刺激。各組細(xì)胞于41℃恒溫水箱中分別培養(yǎng)0.5、1、1.5、2h后添加NE刺激,采用細(xì)胞表面積收縮比(%)反映細(xì)胞對NE的反應(yīng)性。結(jié)果與對照組比較,無論是給予低濃度或常規(guī)濃度NE刺激,HS組細(xì)胞熱打擊1h時對于NE的反應(yīng)性均明顯增高,2h時對于NE的反應(yīng)性則明顯降低(P0.05或P0.01);SOD+HS組細(xì)胞對NE的反應(yīng)性在熱打擊至2h后均出現(xiàn)明顯下降(P0.05或P0.01)。對照組及HS組細(xì)胞對不同濃度NE刺激的反應(yīng)性差異無統(tǒng)計學(xué)意義(P0.05),SOD+HS組細(xì)胞在熱打擊過程中對常規(guī)濃度NE的反應(yīng)性明顯高于對低劑量NE的反應(yīng)性(P0.05或P0.01)。無論給予常規(guī)濃度或低濃度NE刺激,SOD+HS組細(xì)胞對NE的反應(yīng)性均低于HS組(P0.05或P0.01)。結(jié)論熱打擊過程中,HUASMCs對NE的反應(yīng)性呈時相性變化�？寡趸瘎┰谥惺钤缙诳赡芸梢约m正HUASMCs對NE的過反應(yīng)現(xiàn)象,但在熱打擊中后期,抗氧化劑并不能改善其反應(yīng)性的下降。
[Abstract]:Aim to study the effects of antioxidants on the contractile response of human umbilical artery smooth muscle cells (HUASMCs) during thermal shock. Methods HUASMCs were divided into three groups: control group, heat shock group (HS group) and superoxide dismutase (SOD HS) pretreatment group (SOD HS group). Each group was stimulated with low concentration of NE (0.05 mg / L) and routine concentration of NE (1.0 mg / L). The cells in each group were cultured in a constant temperature water tank at 41 鈩，

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