發(fā)布時(shí)間：2018-07-05 03:49

  本文選題：急性心肌梗死 + 骨髓基質(zhì)干細(xì)胞�。� 參考：《大連醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的:探討經(jīng)靜脈移植自體骨髓基質(zhì)干細(xì)胞治療急性心肌梗死對新西蘭兔心肌組織細(xì)胞Toll樣受體4蛋白(Toll like receptor4，TLR4)表達(dá)情況的影響。 方法:將60只健康新西蘭兔隨機(jī)分為假手術(shù)組、手術(shù)組及治療組3組，每組20只。手術(shù)組及治療組新西蘭兔順次開胸,結(jié)扎左冠狀動(dòng)脈前降支中部1/2處建立急性心肌梗死動(dòng)物模型，，假手術(shù)組開胸于相同部位穿入手術(shù)縫線，但不結(jié)扎。制膜后第2周，治療組經(jīng)耳緣靜脈植入自體骨髓基質(zhì)干細(xì)胞，手術(shù)組及假手術(shù)組植入與骨髓基質(zhì)干細(xì)胞相等量的磷酸緩沖液（PBS）；移植4周后分別取出各組心臟，通過蛋白免疫印跡技術(shù)（Western-blotting）檢測三組心肌組織中TLR4表達(dá)的情況。通過Gel-Pro Analyzer軟件測定蛋白條帶灰度值，計(jì)算TLR4的相對定量。組間比較采用秩和檢驗(yàn)，數(shù)據(jù)采用中位數(shù)、百分位數(shù)（上四分位數(shù)，下四分位數(shù)）表示，以P0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果:手術(shù)組缺血壞死心肌組織內(nèi)的TLR4表達(dá)明顯高于假手術(shù)組（P＜0.05），治療組心肌組織內(nèi)的TLR4的表達(dá)低于手術(shù)組（P＜0.05），但高于假手術(shù)組（P＜0.05）。 結(jié)論:1.急性心肌梗死發(fā)生后TLR4的表達(dá)迅速上調(diào)，提示TLR4在急性心肌梗死的發(fā)病過程中具有重要的意義，其可能作為重要的炎性受體介導(dǎo)心肌組織的缺血炎性損傷；2.自體骨髓基質(zhì)干細(xì)胞移植于發(fā)生急性心肌梗死的心肌組織后可以生存、分化；3.經(jīng)耳緣靜脈移植自體骨髓基質(zhì)干細(xì)胞可以治療急性心肌梗死，其機(jī)制之一可能與抑制TLR4所介導(dǎo)的炎性反應(yīng)有關(guān)，其可在一定程度上抑制參與TLR4介導(dǎo)的炎癥反應(yīng)的炎性因子的合成及釋放。
[Abstract]:Aim: to investigate the effect of intravenous transplantation of autologous bone marrow stromal cells (BMSCs) on the expression of Toll like receptor 4 (TLR4) in myocardial tissue of New Zealand rabbits. Methods: 60 healthy New Zealand rabbits were randomly divided into sham operation group, operation group and treatment group with 20 rabbits in each group. New Zealand rabbits in operation group and treatment group were treated with thoracotomy sequentially, and acute myocardial infarction model was established by ligating left anterior descending coronary artery at 1 / 2 of the middle of left coronary artery. In sham-operation group, the thoracotomy was perforated into the suture of operation at the same position, but not ligated. At the second week after membrane preparation, autologous bone marrow stromal stem cells were implanted through the auricular vein in the treatment group, and PBS was implanted in the operation group and the sham operation group in the same amount as the bone marrow stromal stem cells. The hearts of each group were removed after 4 weeks of transplantation. The expression of TLR4 was detected by Western blotting. The relative quantification of TLR4 was calculated by Gel-Pro Analyzer software. The data were expressed as median, percentile (upper quartile, lower quartile), and the difference was statistically significant (P0.05). Results: the expression of TLR4 in ischemic necrotic myocardium in operation group was significantly higher than that in sham operation group (P < 0.05). The expression of TLR4 in myocardial tissue in treatment group was lower than that in operation group (P < 0.05), but higher than that in sham operation group (P < 0.05). Conclusion 1. The expression of TLR4 was upregulated rapidly after acute myocardial infarction, suggesting that TLR4 may play an important role in the pathogenesis of acute myocardial infarction and may act as an important inflammatory receptor to mediate myocardial ischemic inflammatory injury. Autologous bone marrow stromal stem cells can survive after transplantation in myocardial tissue with acute myocardial infarction. Autologous bone marrow stromal stem cells can be transplanted into auricular vein to treat acute myocardial infarction. One of the mechanisms may be related to the inhibition of TLR4-mediated inflammatory response. It can inhibit the synthesis and release of inflammatory factors involved in TLR 4-mediated inflammatory response to some extent.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R542.22

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