發(fā)布時(shí)間：2018-06-24 17:28

  本文選題：失血性休克 + 腎上腺素��； 參考：《大連醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的： 本研究旨在探討腎上腺素β2受體調(diào)控對(duì)失血性休克大鼠肝臟和骨骼肌PGC-1表達(dá)及預(yù)后的影響。 方法： 102只大鼠建立失血性休克—復(fù)蘇模型（放血量按22.5ml/kg體重計(jì)算，放血時(shí)間在10分鐘內(nèi)完成，維持30分鐘)。隨機(jī)分為對(duì)照組（sham）、休克組（SR）、干預(yù)組（PR）：對(duì)照組不進(jìn)行任何操作；休克組建立失血性休克模型30分鐘后進(jìn)行復(fù)蘇；干預(yù)組建立失血性休克模型后立即靜脈給予ICI118.551，，30分鐘后進(jìn)行復(fù)蘇。觀察并記錄各組大鼠的血流動(dòng)力學(xué)變化：平均動(dòng)脈壓（MAP）和心率（HR）；記錄大鼠生存時(shí)間并計(jì)算72小時(shí)生存率；使用定量PCR法和Western blotting法分別檢測(cè)各組2小時(shí)、4小時(shí)、8小時(shí)和24小時(shí)肝臟和骨骼肌組織PGC-1mRNA和蛋白的表達(dá)變化情況。 結(jié)果： 1、生存率：三組大鼠72小時(shí)生存率分別為100％、50%、80%。平均生存時(shí)間分別為72.00±0.00h、48.00±8.25h、62.40±3.40h。休克組和干預(yù)組間比較有統(tǒng)計(jì)學(xué)差異（p0.05）。 2、血流動(dòng)力學(xué)：休克組大鼠MAP在各時(shí)間點(diǎn)上的變化與干預(yù)組比較無統(tǒng)計(jì)學(xué)差異（p＞0.05）。休克組大鼠HR在各時(shí)間點(diǎn)上的變化與干預(yù)組比較沒有統(tǒng)計(jì)學(xué)差異（p＞0.05）。 3、肝臟和骨骼肌組織PGC-1mRNA表達(dá)變化：干預(yù)組在復(fù)蘇后2小時(shí)PGC-1mRNA表達(dá)顯著低于休克組，與休克組比較有統(tǒng)計(jì)學(xué)差異（p0.05）。其它時(shí)間點(diǎn)休克組和干預(yù)組PGC-1mRNA表達(dá)比較無統(tǒng)計(jì)學(xué)差異（p0.05）。 4、肝臟和骨骼肌組織PGC-1蛋白表達(dá)變化：干預(yù)組在復(fù)蘇后2小時(shí)PGC-1表達(dá)顯著低于休克組，與休克組比較有統(tǒng)計(jì)學(xué)差異（p0.05）。其它時(shí)間點(diǎn)休克組和干預(yù)組PGC-1蛋白表達(dá)比較無統(tǒng)計(jì)學(xué)差異（p0.05）。 結(jié)論： 1、阻斷腎上腺素β2受體可以提高失血性休克大鼠72小時(shí)生存率。 2、阻斷腎上腺素β2受體可短暫下調(diào)失血性休克大鼠肝組織和骨骼肌組織PGC-1基因和蛋白的表達(dá)。 3、阻斷腎上腺素β2受體可能通過下調(diào)PGC-1的表達(dá)，減少失血性休克大鼠的能量消耗，進(jìn)而提高生存率。
[Abstract]:Aim: to investigate the effects of adrenaline 尾 2 receptor regulation on the expression and prognosis of PGC-1 in liver and skeletal muscle of hemorrhagic shock rats. Methods: the model of hemorrhagic shock and resuscitation was established in 102 rats (the blood volume was calculated according to 22.5ml/kg body weight, the bleeding time was completed in 10 minutes and maintained for 30 minutes). (sham), shock group (SR) was randomly divided into control group (SR), intervention group (PR): the control group did not perform any operation, the shock group established hemorrhagic shock model 30 minutes after resuscitation, and the intervention group was resuscitated immediately after the establishment of hemorrhagic shock model by intravenous administration of ICI 118.551h for 30 minutes. The mean arterial pressure (map) and heart rate (HR), survival time and 72 hour survival rate were observed and recorded. The expression of PGC-1 mRNA and protein in liver and skeletal muscle were detected by quantitative PCR and Western blotting. Results: 1, survival rate: the 72-hour survival rate of the three groups was 100 and 50 / 80, respectively. The mean survival time was 72.00 鹵0.00h, 48.00 鹵8.25h, 62.40 鹵3.40h, respectively. There was significant difference between shock group and intervention group (p0.05). 2Hemodynamics: there was no significant difference in map between shock group and intervention group at each time point (p > 0.05). The changes of HR in shock group were not significantly different from those in intervention group (p > 0.05). 3. The expression of PGC-1 mRNA in liver and skeletal muscle was significantly lower in intervention group than that in shock group at 2 hours after resuscitation. Compared with shock group, there was statistical difference (p0.05). There was no significant difference in PGC-1mRNA expression between shock group and intervention group at other time points (p0.05). 4. The expression of PGC-1 protein in liver and skeletal muscle was significantly lower than that in shock group at 2 hours after resuscitation. Compared with shock group, there was statistical difference (p0.05). There was no significant difference in PGC-1 protein expression between shock group and intervention group at other time points (p0.05). Conclusion: 1. Blocking adrenaline 尾 2 receptor can improve 72 hour survival rate of hemorrhagic shock rats. 2. Blocking adrenaline 尾 2 receptor can temporarily downregulate liver tissue and skeletal muscle of hemorrhagic shock rats. The expression of PGC-1 gene and protein. 3. Blocking adrenaline 尾 2 receptor may down-regulate the expression of PGC-1. The energy consumption of hemorrhagic shock rats was reduced and the survival rate was improved.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R459.7

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相關(guān)期刊論文 前1條

1 趙自剛,張靜,牛春雨;多器官功能障礙綜合征發(fā)病機(jī)制的某些研究進(jìn)展[J];微循環(huán)學(xué)雜志;2004年03期

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