發(fā)布時間：2018-05-28 20:07

  本文選題：百草枯中毒 + 烏司他丁�。� 參考：《吉林大學》2013年碩士論文

【摘要】：目的在臨床工作中，百草枯中毒患者病死率非常高，其治療辦法很少治療方向也不明確。本文從實驗動物角度去探討在急性百草枯中毒大鼠經(jīng)過烏司他�。║TI）和Sal治療后，肺細胞中自噬和凋亡的變化。 方法選用由吉林大學實驗動物中心提供Wistar大鼠350只，體重(250±10)g，雌雄各半�？瞻讓φ战M（Con）按雌雄各半原則隨機選取。一次性給予1ml生理鹽水灌胃，PQ染毒模型一次性給予PQ溶液40mg/kg灌胃造模，造模成功后，按雌雄各半原則隨機將大鼠分為6組，每組各50只。治療方案：Con組每日二次1ml生理鹽水腹腔注射；PQ染毒組每日二次給予1ml生理鹽水腹腔注射；PQ染毒UTI治療組大鼠給予UTI（12萬iu/kg）日二次腹腔注射；Sal（0.5mg/kg）治療組大鼠PQ染毒后1、3、5天，每日一次給予Sa（l0.5mg/kg）1ml腹腔注射；PQ染毒Sa（l1.0mg/kg）治療組大鼠PQ染毒后1、3、5天，每日一次給予Sal（1.0mg/kg）腹腔注射；PQ染毒UTI＋Sal（0.5mg/kg）治療組，給予UTI（12萬iu/kg），每日二次腹腔注射，在1、3、5天給予一次Sal（0.5mg/kg）腹腔注射；PQ染毒UTI＋Sal（1.0mg/kg）治療組，給予UTI（12萬iu/kg），每日二次腹腔注射，， PQ中毒后1、3、5天，每日一次給予Sal（1.0mg/kg）腹腔注射。染毒后第7天留取肺臟組織，行肺部HE染色觀察肺組織形態(tài)，免疫組織化學法檢測肺組織LC-3和Bcl-2的表達， Westernblot檢測肺組織中LC-3、Bax、Bcl-2和Caspase3蛋白的表達，行統(tǒng)計學分析。 結(jié)果： 1HE染色結(jié)果顯示：Con組大鼠肺組織形態(tài)正常；PQ染毒組肺組織結(jié)構部分破壞，部分肺泡壁塌陷，部分肺泡腔擴大，局部炎細胞浸潤�？梢姺闻莞麸@著增厚，明顯的局部出血；UTI治療組肺組織結(jié)構破壞較輕；其他組別均有不同程度的破壞。 2與PQ組相比，PU組中LC3A/B蛋白含量表達增高有統(tǒng)計學意義（P0.05)，PS5組和PS10組中LC3A/B蛋白含量表達降低有統(tǒng)計學意義（P0.05)，PUS5組和PUS10組中LC3A/B蛋白含量表達無明顯變化；PU組、PS5組、 PS10組、PUS5組和PUS10組中Bcl-2/Bax蛋白含量表達增高有統(tǒng)計學意義（P0.05)；與PU組相比，PUS5組和PUS10組中LC3A/B和Bcl-2/Bax蛋白含量表達降低有統(tǒng)計學意義（P0.05)；與PS5相比，PUS5組LC3A/B和Bcl-2/Bax蛋白含量表達增高有統(tǒng)計學意義（P0.05)。與PS10相比，PUS10組LC3A/B和Bcl-2/Bax蛋白含量表達增高有統(tǒng)計學意義（P0.05) 3與Con組相比，PQ組Cleaved-Caspase3蛋白含量表達增高有統(tǒng)計學意義（P0.05)。與PQ組相比，PU組Cleaved-Caspase3蛋白含量表達顯著降低有統(tǒng)計學意義（P0.01)， PS5組、PS10組、PUS5組和PUS10組中Cleaved-Caspase3蛋白含量表達降低有統(tǒng)計學意義（P0.05)； 4免疫組化結(jié)果顯示：與相比Con組相比， PQ組LC3和Bcl-2蛋白表達量明顯降低有統(tǒng)計學意義（P0.05）；與PQ組相比， PU組、PS5組、 PS10組、PUS5組和PUS10組LC3和Bcl-2蛋白表達量均有不同程度的改變；結(jié)論：急性PQ中毒大鼠肺組織發(fā)生內(nèi)質(zhì)網(wǎng)應激-自噬。UTI治療急性PQ中毒時是通過增加肺組織自噬的發(fā)生，增加Bcl-2的表達和減少Caspase3依賴途徑的凋亡來保護肺組織。而Sal降低了急性PQ中毒大鼠肺組織中細胞自噬的發(fā)生，增高了Bcl-2的表達，具體作用機制還有待于探討。
[Abstract]:Objective in clinical work, the mortality of paraquat poisoning patients is very high, and the treatment methods are not very clear. In this paper, the changes of autophagy and apoptosis in lung cells after acute paraquat poisoning in rats with UTI and Sal were studied in this paper.
Methods 350 Wistar rats were selected from the experimental animal center of Jilin University, the body weight (250 + 10) g and male and female were half. The blank control group (Con) was randomly selected according to the male and female half principle. The 1ml saline was given to the stomach in one time. The PQ poisoning model was given to the PQ solution by 40mg/kg to make the gastric mold at one time. After the model was successful, the rats were randomly selected according to the male and male principle. The rats were divided into 6 groups, 50 in each group. The treatment regimen was treated in group Con two times daily 1ml saline intraperitoneal injection, and 1ml saline was given to PQ in the group of PQ for UTI (120 thousand iu/kg) for two intraperitoneal injection; Sal (0.5mg/kg) group was given 1,3,5 days after PQ poisoning. G) 1ml intraperitoneal injection; PQ infected Sa (l1.0mg/kg) group was given Sal (1.0mg/kg) intraperitoneal injection once a day after PQ exposure; PQ was administered to UTI + Sal (120 thousand) treatment group, given a daily intraperitoneal injection. UTI (120 thousand iu/kg), two times daily intraperitoneal injection, PQ poisoning after 1,3,5 days, Sal (1.0mg/kg) intraperitoneal injection once a day. After seventh days of exposure to lung tissue, lung HE staining observation of lung tissue morphology, immunohistochemical method for detection of lung tissue LC-3 and Bcl-2, Westernblot test LC-3, Bax, Bax, Bax, and eggs in lung tissue White expression, statistical analysis.
Result錛

本文編號：1948009