本文選題：膿毒癥 + 心肌功能障礙　； 參考：《中國病理生理雜志》2017年05期

【摘要】：目的:利用輕度盲腸結(jié)扎穿孔術(shù)(CLP)復制膿毒癥大鼠模型,比較膿毒癥大鼠心功能與其它器官功能障礙發(fā)生的時間。方法:將大鼠隨機分為假手術(shù)組和盲腸結(jié)扎穿孔術(shù)組,分別于手術(shù)后6 h、9 h和12 h用Langendorff裝置檢測大鼠心肌收縮與舒張功能,并測定心肌腫瘤壞死因子α(TNF-α)、細胞間黏附分子1(ICAM-1)和血管內(nèi)皮細胞黏附因子1(VCAM-1)的表達;同時檢測血清TNF-α水平以及大鼠肝、腎功能和肺濕/干重比值。結(jié)果:輕度CLP術(shù)后10 d,大鼠的死亡率為26.7%。CLP術(shù)后9 h與12 h左心室內(nèi)壓最大上升與下降速率顯著低于假手術(shù)組;CLP術(shù)后6 h,心肌TNF-α、ICAM-1和VCAM-1的mRNA表達顯著高于假手術(shù)組,CLP術(shù)后9 h心肌TNF-α和VCAM-1的蛋白表達顯著升高。CLP術(shù)后9 h血清天冬氨酸氨基轉(zhuǎn)移酶活性和12 h丙氨酸氨基轉(zhuǎn)移酶活性高于假手術(shù)組。而術(shù)后6 h、9 h和12 h,大鼠血尿素氮水平、肺濕/干重比與相應(yīng)時點的假手術(shù)組比較,差異沒有統(tǒng)計學顯著性。結(jié)論:輕度CLP模型大鼠在膿毒癥發(fā)生后6 h心肌炎癥因子表達增多,9 h后即發(fā)生心肌內(nèi)在收縮與舒張功能障礙,其發(fā)生早于肝、腎功能損傷。
[Abstract]:Aim: to establish sepsis rat model with mild cecal ligation and perforation, and compare the time between cardiac function and other organ dysfunction in septic rats. Methods: rats were randomly divided into sham operation group and cecal ligation and perforation group. Myocardial contractile and diastolic function were measured by Langendorff device at 6 h and 12 h after operation respectively. The expression of TNF- 偽, ICAM-1 and VCAM-1 were measured, and the levels of TNF- 偽 in serum, liver and kidney function and lung wet / dry weight ratio were also measured. Results: at 10 days after mild CLP, the death rate of rats was significantly lower than that of sham operation group at 9 and 12 hours after 26.7%.CLP. The mRNA expression of TNF- 偽 ICAM-1 and VCAM-1 in myocardium was significantly higher than that in sham operation group. The expression of TNF- 偽 and VCAM-1 in myocardium increased significantly at 9 h after operation. The activities of serum aspartate aminotransferase and alanine aminotransferase at 9 h after operation were significantly higher than those in sham operation group. However, there was no significant difference in blood urea nitrogen level and lung wet / dry weight ratio between sham operation group and sham operation group at 6 h and 12 h after operation. Conclusion: the expression of myocardial inflammatory factors in mild CLP model rats increased at 6 h after sepsis and 9 h after sepsis, the myocardial intrinsic systolic and diastolic dysfunction occurred, which occurred earlier than that of liver and kidney function injury.
【作者單位】： 暨南大學基礎(chǔ)醫(yī)學院病理生理學系國家中醫(yī)藥管理局三級科研實驗室;
【基金】：國家自然科學基金資助項目(No.81372028) 廣東省教育廳學科建設(shè)科技創(chuàng)新項目(No.2013KJCX0019) 廣州市科技計劃項目(No.201508020005) 暨南大學高水平大學建設(shè)醫(yī)學科學研究與應(yīng)用組團聯(lián)合研究項目(No.88016013045)
【分類號】：R-332;R459.7

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