本文選題：藥物性亞冬眠 + 冬眠合劑�。� 參考：《鄭州大學(xué)》2013年碩士論文

【摘要】：目的 通過觀察藥物性亞冬眠對大鼠急性腦梗塞腦組織中基質(zhì)金屬蛋白激酶-9(Matrix Metalloproteinase-9, MMP-9)和血小板活化因子(Platelet-Activating Factor,PAF)表達(dá)的變化,探討藥物性亞冬眠對大鼠急性腦梗塞的影響及可能的作用機(jī)制。 方法 成年雄性SD大鼠共63只,隨機(jī)分成3組：對照組(只分離頸內(nèi)、外動脈,不做栓塞處理)、模型組(通過改良Longa線栓法來制備大鼠大腦中動脈局灶性腦梗塞模型)及亞冬眠組(改良Longa線栓法造模成功后,給予冬眠合劑肌注),其中對照組及模型組在與亞冬眠組相同時間肌注等量生理鹽水。三組分別于缺血后4h、12h、24h進(jìn)行神經(jīng)功能評分,各時間點(diǎn)取7只大鼠心臟灌洗后快速斷頭取腦進(jìn)行HE染色,觀察局部腦梗死的大體形態(tài),并應(yīng)用免疫組化Sp法檢測PAF、MMP-9的表達(dá)。 結(jié)果 1.神經(jīng)功能缺損評分情況：模型組的大鼠神經(jīng)功能缺損最為嚴(yán)重,其各時間點(diǎn)缺損評分情況與對照組相比較有顯著性差異(P0.05);對照組大鼠無神經(jīng)功能缺損,評分為0；而亞冬眠組大鼠神經(jīng)功能缺損評分情況較模型組降低,其各時間點(diǎn)與模型組及對照組比較均有顯著性差異(P0.05); 2.HE染色大體觀察結(jié)果：模型組與亞冬眠組大鼠病灶腦組織的腫脹最為明顯,亞冬眠組較模型組情況有所減輕,而對照組未見腦組織腫脹情況。進(jìn)行HE染色之后在光鏡下觀察：對照組大鼠腦的海馬區(qū)神經(jīng)細(xì)胞能整齊排列,且有清晰的輪廓,密集,神經(jīng)元胞漿豐富,淡染,胞核多位于細(xì)胞中央,核仁清晰；模型組海馬區(qū)神經(jīng)細(xì)胞輪廓模糊,排列不規(guī)則紊亂,數(shù)量減少,且間質(zhì)水腫疏松,胞漿濃縮紅染,分界不清,胞核皺縮或溶解甚至碎裂；亞冬眠組神經(jīng)細(xì)胞與模型組相比結(jié)構(gòu)尚清晰,間質(zhì)水腫明顯減輕,胞核與胞質(zhì)尚能分清。 3.PAF、MMP-9免疫組化結(jié)果：各組均可見PAF、MMP-9陽性細(xì)胞表達(dá),都在胞質(zhì)中,呈現(xiàn)棕黃色染色,陽性細(xì)胞個數(shù)越多、染色越深,提示免疫反應(yīng)越強(qiáng),LOD值越大,則因子表達(dá)越多；在三組組內(nèi)比較可見：各時間點(diǎn)PAF于腦缺血4h內(nèi)已達(dá)較高水平,12h、24h值相對于4h未見明顯差異(P0.05);MMP-9于腦缺血4h內(nèi)表達(dá)已開始增高,于12h、24h持續(xù)升高。三組間比較可見：PAF、MMP-9于模型組及亞冬眠組各時間點(diǎn)與對照組比較均明顯增高(P0.05);亞冬眠組各時間點(diǎn)與模型組相比,表達(dá)明顯減少(P0.05)。 結(jié)論 藥物性亞冬眠對大鼠急性腦梗塞損傷有腦保護(hù)作用,其作用機(jī)制可能通過減少鈣離子過量內(nèi)流、炎性介質(zhì)的生成,進(jìn)而減輕由此造成的代謝紊亂連鎖反應(yīng)的發(fā)生有關(guān)。
[Abstract]:Purpose To investigate the effect of drug subhibernation on the expression of matrix metalloproteinase-9 (MMP-9) and platelet activating factor Platelet-Activating factor-PAF (PAF) in rats with acute cerebral infarction, the effects of drug subhibernation on acute cerebral infarction and its possible mechanism were investigated. Method 63 adult male Sprague-Dawley rats were randomly divided into three groups: control group (internal and external carotid artery separation, Without embolization, the model group (model of focal cerebral infarction in the middle cerebral artery of rats by modified Longa thread occlusion method) and the subhibernating group (modified Longa thread embolization method were successfully established). The control group and model group were intramuscularly injected with normal saline at the same time as the subhibernation group. The neurological function scores were evaluated at 4 hours and 12 hours after ischemia in the three groups. The brain of 7 rats were quickly severed after heart lavage at each time point for HE staining. The gross morphology of local cerebral infarction was observed and the expression of PAFMP-9 was detected by immunohistochemical Sp method. Result 1. The neurological deficit score in the model group was the most serious, and there was significant difference between the model group and the control group (P 0.05). The score of nerve function defect in the subhibernation group was lower than that in the model group, and there was significant difference between the model group and the control group at each time point (P 0.05). The results of 2.HE staining showed that the swelling of brain tissue in the model group and the subhibernation group was the most obvious, and that in the subhibernating group was less than that in the model group, but there was no swelling in the brain tissue in the control group. After HE staining, the hippocampal neurons in the control group were neatly arranged, with clear outline, dense, rich cytoplasm and light staining. The nucleus was located in the center of the cell and the nucleolus was clear. In the model group, the contour of hippocampal neurons was fuzzy, the number of neurons was irregular, the number was decreased, the interstitial edema was loose, the cytoplasm was concentrated and red stain, the boundary was not clear, the nucleus was crumpled or dissolved or even broken. Compared with the model group, the nerve cells in the subhibernation group had clear structure, the interstitial edema was alleviated, and the nucleus and cytoplasm could be distinguished. 3. Immunohistochemical results of PAF- MMP 9: PAF- MMP 9 positive cells were expressed in cytoplasm. The more the number of positive cells was, the deeper the staining was, which indicated that the stronger the immune response was, the greater the expression of factors was. The comparison among the three groups showed that the expression of PAF at each time point reached a higher level within 4 h after cerebral ischemia than at 4 h after cerebral ischemia, and the expression of MMP 9 began to increase within 4 h after cerebral ischemia and continued to increase at 12 h after ischemia. Compared with the control group, the expression of MMP 9 in the model group and the subhibernation group was significantly higher than that in the control group, and the expression of MMP 9 in the subhibernation group was significantly lower than that in the model group. Conclusion Drug subhibernation has brain protective effect on acute cerebral infarction injury in rats. The mechanism may be related to reducing the formation of inflammatory mediators and reducing the chain reaction of metabolic disorder caused by calcium excess influx.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R743.33

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