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  本文選題：急性腦缺血 + 胱抑素C��； 參考：《寧夏醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:了解急性腦缺血患者血清胱抑素(Cys C)表達水平及其不同結(jié)構(gòu)形式(單體/寡聚體)的變化;分析不同程度急性腦缺血(輕、中度)血清Cys C表達水平變化;為下一步研究Cys C不同結(jié)構(gòu)形式(單體及寡聚體)對缺氧狀態(tài)下神經(jīng)細胞的增殖及凋亡的影響提供前期研究基礎(chǔ)。方法:選取寧夏醫(yī)科大學(xué)總醫(yī)院神經(jīng)內(nèi)科及腦卒中病區(qū)自2016年5月1日-2016年8月31日收住院的急性腦梗死患者32例為病例組;根據(jù)入院當天NIHSS評分結(jié)果將病例組分為輕、中度。選取同期在本院體檢中心體檢的健康人群26例作為對照組。分別收集兩組研究對象的臨床資料,于空腹8小時后采血,用酶聯(lián)免疫吸附實驗(ELISA)法測定血清Cys C表達水平,并用western blotting法測定Cys C不同結(jié)構(gòu)形式。結(jié)果:急性腦缺血組與對照組臨床資料,性別、年齡、血壓、血糖、吸煙、飲酒史進行比較,差異均無統(tǒng)計學(xué)意義(P0.05);急性腦缺血組患者血清Cys C水平[(1.086±0.658)mg/L]高于對照組[(0.687±0.099)mg/L],兩組比較差異具有統(tǒng)計學(xué)意義(t=3.056,p=0.003);急性腦缺血組患者血清Cys C寡聚體率(87.5%)明顯高于對照組(19.2%),兩組相比差異有統(tǒng)計學(xué)意義(X2=27.261,P0.001)。比較分析不同程度急性腦缺血患者(輕、中度)Cys C表達水平,并通過Wilcoxon秩和檢驗提示兩組間比較差異無統(tǒng)計學(xué)意義。結(jié)論:急性腦缺血患者血清Cys C表達水平升高;急性腦缺血患者血清Cys C表達水平升高是以寡聚體升高為主;本研究中急性腦缺血嚴重程度與Cys C表達水平無明顯關(guān)系。
[Abstract]:Objective: to investigate the changes of serum cystatin C (Cys C) expression and its different structural forms (monomer / oligomer) in patients with acute cerebral ischemia, and to analyze the changes of serum Cys C expression in patients with acute cerebral ischemia (mild and moderate). It provides a basis for further study on the effects of different structural forms of Cys C (monomers and oligomers) on the proliferation and apoptosis of neurons under hypoxia. Methods: Thirty-two patients with acute cerebral infarction admitted from May 1, 2016 to August 31, 2016 in the Department of Neurology and Stroke in the General Hospital of Ningxia Medical University were selected as the case group, and the patients were divided into mild and moderate groups according to the NIHSS score on the day of admission. 26 healthy people were selected as control group. The clinical data of the two groups were collected respectively. Blood samples were collected 8 hours after fasting. The expression of Cys C in serum was determined by Elisa and the different structural forms of Cys C were determined by western blotting method. Results: the clinical data, sex, age, blood pressure, blood glucose, smoking and drinking history were compared between the acute cerebral ischemia group and the control group. The serum Cys C level in acute cerebral ischemia group [1.086 鹵0.658)mg/L] was higher than that in control group [0.687 鹵0.099)mg/L], the difference between the two groups was statistically significant, and the serum Cys C oligomer rate in acute cerebral ischemia group (87.5%) was significantly higher than that in control group (19.2g%). The difference between the two groups was statistically significant. The expression of Cys C in patients with different degrees of acute cerebral ischemia (mild and moderate) was compared and analyzed. The results of Wilcoxon rank sum test showed that there was no significant difference between the two groups. Conclusion: the expression of Cys C in the serum of patients with acute cerebral ischemia is higher than that of the patients with acute cerebral ischemia, and the level of Cys C in the patients with acute cerebral ischemia is mainly increased by oligodeoxynucleotides. There is no significant correlation between the severity of acute cerebral ischemia and the expression of Cys C in this study.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743.3

【參考文獻】

相關(guān)期刊論文 前10條

1 于利萍;石正洪;;血清胱抑素C水平在缺血性卒中患者急性期及其亞型中的變化和意義[J];中國腦血管病雜志;2016年04期

2 劉Z，

本文編號：1842458