本文選題：垂體中葉素 + IMD　； 參考：《青島大學(xué)》2015年碩士論文

【摘要】：目的:早期檢測(cè)不同嚴(yán)重程度胰腺炎大鼠血清垂體中葉素(Intermedin,IMD)含量,探究血清IMD對(duì)急性胰腺炎大鼠的早期預(yù)測(cè)作用。方法:SD大鼠120只,隨機(jī)分成4組:其中對(duì)照手術(shù)組(NS,n=30)、輕度急性胰腺炎組(mild acute pancreatitis,MAP,n=30)、重癥急性胰腺炎組(severe acute pancreatitis,SAP,n=30)、SAP+烏司他丁干預(yù)組別(SAP+Ulinastatin,UTI,n=30)。急性胰腺炎大鼠造模成功后分別在3小時(shí)、6小時(shí)和12小時(shí)處死而分別采集血樣和胰腺組織,運(yùn)用ELISA法檢測(cè)血清IMD含量、胰腺組織進(jìn)行病理學(xué)評(píng)分和免疫組化定性檢測(cè)胰腺組織IMD表達(dá)情況。結(jié)果:MAP組、SAP組和SAP+Ulinastatin組血清中IMD含量顯著降低于NS組,差異具有統(tǒng)計(jì)學(xué)意義(F=2.062,P0.05):MAP組胰腺炎大鼠血清IMD含量比SAP組僅輕度增加,SAP+Ulinastatin組比SAP組血清IMD含量輕度增加;MAP組、SAP組和SAP+Ulinastatin組隨著時(shí)間的延長(zhǎng)血清IMD含量降低;MAP組、SAP組和SAP+Ulinastatin組病理學(xué)評(píng)分各組間差異顯著,隨著嚴(yán)重程度的加重而評(píng)分增加,具有統(tǒng)計(jì)學(xué)意義(F=14.152,P0.05);各組胰腺炎大鼠組織病理學(xué)評(píng)分隨著時(shí)間的延長(zhǎng)而增加。MAP組別3小時(shí)、6小時(shí)和12小時(shí)血清IMD含量與胰腺炎組織病理學(xué)評(píng)分間存在負(fù)相關(guān)(r3=-0.637,P0.05r6=-0.375,P0.05;r12=0.261,P0.05);SAP組別3小時(shí)、6小時(shí)和12小時(shí)血清IMD含量與胰腺炎組織病理學(xué)評(píng)分間存在負(fù)相關(guān)(r3=-0.52,P0.05 r6=-0.17,P0.05;r12=-0.03,P0.05).大鼠胰腺組織免疫組化結(jié)果顯示大鼠胰腺組織中存在IMD的表達(dá)。結(jié)論:IMD對(duì)胰腺組織可能具有保護(hù)重要作用,早期檢測(cè)血清IMD對(duì)大鼠胰腺炎診斷具有重要價(jià)值。目的:探究垂體中葉素聯(lián)合降鈣素原在急性胰腺炎患者血清中的動(dòng)態(tài)改變及臨床意義。方法:根據(jù)急性胰腺炎診斷指南,將急性胰腺炎患者分為輕癥胰腺炎組(MA P組,n=60)及重癥胰腺炎(SAP組,n=30)。檢測(cè)MAP組和SAP組患者在入院24小時(shí)、48小時(shí)、72小時(shí)以及第7天時(shí)血清IMD和PCT含量,同時(shí)患者入院24小時(shí)內(nèi)進(jìn)行APACHEⅡ評(píng)分;將同時(shí)間的健康體檢人群作為正常對(duì)照組(NS組,n=30);比較血清IMD和PCT的改變以及差異性。將血清IMD和PCT、IMD和APACHEⅡ評(píng)分以及PCT和APACHEⅡ評(píng)分進(jìn)行相關(guān)性分析。結(jié)果:MAP組和SAP組PCT隨著時(shí)間的推移,PCT含量逐漸升高,差異顯著(P0.05),SAP組PCT含量明顯高于MAP組;MAP組和SAP組IMD含量隨著時(shí)間的推移,IMD含量逐漸降低,差異顯著(P),SAP組IMD含量明顯低于MAP組,差異顯著(p0.05);血清IMD含量與PCT呈現(xiàn)負(fù)相關(guān),對(duì)急性胰腺診斷具有一定價(jià)值;MAP組和SAP組血清IMD含量與APACHEⅡ評(píng)分均呈負(fù)相關(guān)(rs0,p0.05).MAP組和SAP組血清PCT含量與APACHEⅡ評(píng)分均呈正相關(guān)關(guān)(rs0,p0.05)。結(jié)論:血清IMD、PCT可以作為早期診斷急性胰腺炎的指標(biāo),對(duì)重癥胰腺炎的診斷也具有一定價(jià)值。
[Abstract]:Aim: to investigate the early predictive effect of serum IMD on rats with acute pancreatitis by detecting the content of intermedin in serum of rats with acute pancreatitis. Methods 120 small Sprague-Dawley rats were randomly divided into four groups: control group (n = 30), mild acute pancreatitis group (n = 30) and severe acute pancreatitis group (n = 30). Rats with acute pancreatitis were killed at 3 hours, 6 hours and 12 hours after successful modeling, and blood samples and pancreatic tissues were collected respectively. Serum IMD levels were measured by ELISA method. The expression of IMD in pancreatic tissues was detected by pathological score and immunohistochemical staining. Results the content of IMD in serum of SAP group and SAP Ulinastatin group was significantly lower than that of NS group. The difference was statistically significant (P < 0.05). The serum IMD content of rats in SAP group was only slightly higher than that in SAP group. The serum IMD content in SAP Ulinastatin group was slightly higher than that in SAP group. The serum IMD content in map group and SAP Ulinastatin group decreased with the prolongation of time. There was significant difference between the pathological score of SAP Ulinastatin group and that of SAP Ulinastatin group. As the severity increases, the score increases, The histopathological score of rats with pancreatitis increased with time. There was a negative correlation between the serum IMD content of 3 hours and 12 hours in map group and the histopathological score of pancreatitis. There was a negative correlation between the serum IMD content and the histopathological score of pancreatitis group. There was a negative correlation between the serum IMD content and the histopathological score of pancreatitis at 3 hours, 6 hours and 12 hours, respectively, and there was a negative correlation between the levels of serum IMD and the histopathological score of pancreatitis. Immunohistochemical results showed that there was IMD expression in pancreatic tissue of rats. Conclusion the early detection of serum IMD may play an important role in the protection of pancreatic tissue, and early detection of serum IMD may be of great value in the diagnosis of pancreatitis in rats. Objective: to investigate the dynamic changes and clinical significance of pituitary mesophylloin combined with procalcitonin in serum of patients with acute pancreatitis. Methods: according to the diagnostic guidelines of acute pancreatitis, the patients with acute pancreatitis were divided into mild pancreatitis group (MA P group) and severe pancreatitis group (SAP group). The serum levels of IMD and PCT were measured in MAP group and SAP group at 24 hours, 48 hours, 72 hours and 7 days after admission, and APACHE 鈪，

本文編號(hào)：1797454