本文選題：膿毒癥 + 循環(huán)miRNA��； 參考：《實用醫(yī)學(xué)雜志》2017年24期

【摘要】：目的應(yīng)用微陣列芯片分析技術(shù)篩選膿毒癥診斷和預(yù)后相關(guān)循環(huán)miRNAs,通過實時熒光定量PCR驗證,并應(yīng)用生物信息學(xué)方法預(yù)測膿毒癥相關(guān)靶基因和發(fā)病機(jī)制。方法入選膿毒癥、膿毒癥休克患者及正常人各3例,提取血漿miRNA,分別與第7代人,小鼠和大鼠的miRCURY LNA~(TM)microRNA芯片雜交,掃描信號進(jìn)行差異表達(dá)譜和聚類分析;應(yīng)用qRT-PCR驗證芯片結(jié)果。通過miRanda和Targetscan數(shù)據(jù)庫預(yù)測miRNAs靶基因及KEGG數(shù)據(jù)庫分析其通路。結(jié)果與正常組相比,膿毒癥組有57種miRNAs表達(dá)上調(diào),膿毒癥休克組有11種(fold change≥2.0;P0.05)。miR-519c-5p被用于qRT-PCR驗證,結(jié)果表明其在膿毒癥中表達(dá)明顯高于正常組,差異有統(tǒng)計學(xué)意義(P0.05)。通過生物信息學(xué)方法我們預(yù)測了29個miR-519c-5p相關(guān)靶基因,通過KEGG數(shù)據(jù)庫分析其中有7個作用于膿毒癥相關(guān)通路。最終表明MiR-519c-5p可能正性調(diào)節(jié)細(xì)胞周期調(diào)控基因MAP2K4,通過MAPK信號通路導(dǎo)致血管內(nèi)皮細(xì)胞凋亡。結(jié)論首次證明血漿miR-519c-5p可能可用于膿毒癥診斷并作為可能潛在靶點用于膿毒癥治療。
[Abstract]:Objective to screen circulating miRNAs-associated sepsis by microarray analysis, and to predict the target genes and pathogenesis of sepsis by real-time fluorescence quantitative PCR.Methods Plasma miRNAs were extracted from septic patients, septic shock patients and normal controls, and hybridized with miRCURY LNA~(TM)microRNA microarray of the 7th generation, mice and rats, respectively. The differential expression profile and cluster analysis were performed by scanning signals, and the results were verified by qRT-PCR.MiRNAs target gene and KEGG database were analyzed by miRanda and Targetscan database.Results compared with the normal group, 57 kinds of miRNAs expression were up-regulated in septic group and 11 kinds of change 鈮，

本文編號：1749788