發(fā)布時間：2018-04-13 18:58

  本文選題：三黃燒傷靈 + 休克期��； 參考：《內(nèi)蒙古大學》2013年碩士論文

【摘要】：目的探索外用中藥制劑對嚴重燙傷休克期大鼠內(nèi)臟的保護作用及機制。方法通過血液檢測、組織病理學觀察、免疫組化、ELISA等方法在休克期的8h、24h、48h測定小腸粘膜HSP70；肺組織丙二醛含量、還原型谷胱甘肽含量、肺質(zhì)量(濕)/肺質(zhì)量(干)比；腎功能測定(Bun、Cr)、腎組織髓過氧化物酶(MPO)測定；血清中的TNF-α、IL-6。結(jié)果三黃燒傷靈治療組各時間點HPS70表達均較碘伏治療組明顯升高,燙傷早期Wistar大鼠小腸粘膜組織損傷明顯,三黃燒傷靈Wistar治療組大鼠小腸粘膜組織損傷較碘伏治療組組明顯減輕；三黃燒傷靈治療組Wistar大鼠肺臟中丙二醛含量及還原型谷胱甘肽含量組顯著高于碘伏治療組,肺質(zhì)量(濕)/肺質(zhì)量(干)比顯著低于碘伏治療組；三黃燒傷靈治療組血漿BUN和Cr與碘伏治療組比較有明顯下降,局部髓過氧化物酶活性降低；三黃燒傷靈治療組Wistar大鼠傷后48hTNF-α、IL-6均明顯低于碘伏治療組。結(jié)論三黃燒傷靈、膏狀,做為一種活血化瘀、清熱解毒之中藥,可有效的阻止細菌的侵入,可減輕創(chuàng)面疼痛,是因為其可以有效的阻礙空氣對神經(jīng)末梢的刺激；同時還可以改善創(chuàng)面微循環(huán)、并可促進創(chuàng)面生長。通過組織吸收后改變內(nèi)毒素的結(jié)構,以滅活解毒或降低其毒素。通過減少內(nèi)毒素的釋放,促進內(nèi)毒素的排泄,減弱或消除TNF-α、IL-6的作用,以達到保護內(nèi)臟的目的。
[Abstract]:Objective to explore the protective effect and mechanism of external Chinese medicine preparation on visceral organs in rats with severe scald shock.Methods HSP70 of small intestine mucosa was determined by blood examination, histopathological observation, immunohistochemistry and Elisa at 8 h, 24 h and 48 h after shock, and the contents of malondialdehyde (MDA), glutathione (GSH) and lung mass (wet / dry) were measured in lung tissue.The determination of renal function and myeloperoxidase (MPO) in renal tissue and TNF- 偽 and IL-6 in serum.Results the expression of HPS70 in Sanhuangzangling group was significantly higher than that in iodophor group at each time point, and the mucosal injury of small intestine was obvious in Wistar rats at the early stage of scalding.The intestinal mucosal tissue damage in Sanhuangzangling Wistar group was significantly less than that in iodophor group, and the content of malondialdehyde and glutathione in lung of Wistar rats in Sanhuangzangling group was significantly higher than that in iodophor group.The lung mass (wet / dry) ratio was significantly lower than that in iodophor group, the plasma BUN and Cr in Sanhuang Zangling group were significantly lower than those in iodophor group, and the local myeloperoxidase activity was lower in Sanhuangzangling group than that in iodophor group.The levels of TNF- 偽 IL-6 in Sanhuang Burn Ling group were significantly lower than those in iodophor group at 48 h after injury.Conclusion Sanhuang Burn Ling, as a traditional Chinese medicine of activating blood circulation, removing blood stasis, clearing heat and detoxifying, can effectively prevent bacterial invasion and relieve wound pain, because it can effectively block the stimulation of air to nerve endings.It can also improve wound microcirculation and promote wound growth.Change the structure of endotoxin by tissue absorption to inactivate or detoxify it.By reducing the release of endotoxin, promoting the excretion of endotoxin and attenuating or eliminating the effect of TNF- 偽 and IL-6, the visceral protection can be achieved.
【學位授予單位】：內(nèi)蒙古大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R459.7;R965

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