發(fā)布時(shí)間：2018-04-07 16:41

  本文選題：衛(wèi)星細(xì)胞　切入點(diǎn)：骨骼肌　出處：《中國(guó)組織工程研究》2017年09期

【摘要】：背景:前期研究發(fā)現(xiàn),骨骼肌衛(wèi)星胞移植能夠誘導(dǎo)心肌梗死區(qū)新生血管形成,縮小梗死面積,改善其心功能,但整體效果并不太理想。目的:觀察堿性成纖維細(xì)胞生長(zhǎng)因子基因修飾骨骼肌衛(wèi)星細(xì)胞在急性心肌梗死區(qū)的存活及對(duì)心肌梗死區(qū)血管新生的影響。方法:將18只新西蘭大白兔隨機(jī)分為3組,實(shí)驗(yàn)組、對(duì)照組結(jié)扎冠狀動(dòng)脈左前降支,構(gòu)建急性心肌梗死動(dòng)物模型;空白對(duì)照組只穿線,不結(jié)扎。造模成功即刻,實(shí)驗(yàn)組于局部梗死心肌內(nèi)注射DAPI標(biāo)記的堿性成纖維細(xì)胞生長(zhǎng)因子基因修飾自體骨骼肌衛(wèi)星細(xì)胞懸液50μL,對(duì)照組注射等量DAPI標(biāo)記的自體骨骼肌衛(wèi)星細(xì)胞。細(xì)胞移植4周后取標(biāo)本,觀察心肌梗死區(qū)骨骼肌衛(wèi)星細(xì)胞存活及成纖維細(xì)胞生長(zhǎng)因子基因表達(dá)情況,免疫組織化學(xué)染色檢查心肌梗死區(qū)新生血管形成情況。結(jié)果與結(jié)論:(1)空白對(duì)照組未見DAPI標(biāo)記的細(xì)胞,對(duì)照組及實(shí)驗(yàn)組缺血心肌區(qū)域均可見大量DAPI標(biāo)記的骨骼肌衛(wèi)星細(xì)胞,實(shí)驗(yàn)組還可見大量EGFP-堿性成纖維細(xì)胞生長(zhǎng)因子融合蛋白綠色熒光表達(dá);(2)實(shí)驗(yàn)組、對(duì)照組新生微血管密度多于空白對(duì)照組(P0.05),實(shí)驗(yàn)組新生微血管密度多于對(duì)照組(P0.05);(3)結(jié)果表明,堿性成纖維細(xì)胞生長(zhǎng)因子修飾骨骼肌衛(wèi)星細(xì)胞可在急性心肌梗死區(qū)存活,促進(jìn)心肌梗死區(qū)血管新生。
[Abstract]:Background: previous studies have shown that skeletal muscle satellite cell transplantation can induce neovascularization, reduce infarct size and improve cardiac function in myocardial infarction area, but the overall effect is not very satisfactory.Aim: to investigate the survival and angiogenesis of basic fibroblast growth factor gene modified skeletal muscle satellite cells in acute myocardial infarction (AMI).Methods: eighteen New Zealand white rabbits were randomly divided into three groups: the experimental group, the control group ligated the left anterior descending coronary artery, and the blank control group only threaded and did not ligate the left anterior descending coronary artery.Immediately after the establishment of the model, the experimental group was injected with 50 渭 L DAPI labeled basic fibroblast growth factor gene modified autologous skeletal muscle satellite cell suspension, and the control group was injected with the same amount of DAPI labeled autologous skeletal muscle satellite cell.The survival of skeletal muscle satellite cells and the expression of fibroblast growth factor gene were observed after 4 weeks of cell transplantation. The neovascularization in myocardial infarction area was detected by immunohistochemical staining.Results and conclusion No DAPI labeled cells were found in the blank control group, and a large number of DAPI labeled skeletal muscle satellite cells were found in the ischemic myocardium of the control group and the experimental group.A large number of EGFP-basic fibroblast growth factor fusion protein was also found in the experimental group. The results showed that the neonate microvessel density in the control group was higher than that in the blank control group, and the neonate microvessel density in the experimental group was higher than that in the control group.Basic fibroblast growth factor modified skeletal muscle satellite cells can survive in acute myocardial infarction and promote angiogenesis.
【作者單位】： 北京大學(xué)深圳醫(yī)院心血管外科;
【基金】：深圳市科技計(jì)劃項(xiàng)目(200404104)~~
【分類號(hào)】：R542.22

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