本文選題：甲狀腺素　切入點(diǎn)：重型顱腦損傷　出處：《重慶醫(yī)學(xué)》2017年32期 　論文類型：期刊論文

【摘要】：目的通過(guò)觀察甲狀腺素對(duì)重型顱腦損傷大鼠神經(jīng)細(xì)胞凋亡、血清神經(jīng)元特異性烯醇化酶(NSE)、白細(xì)胞介素(IL)-6的影響及血清FT3、FT4的改變,探討甲狀腺素對(duì)重型顱腦損傷腦組織的保護(hù)作用。方法 90只SD大鼠分為對(duì)照組模型組左甲狀腺素鈉片低劑量組、左甲狀腺素鈉片中劑量組、左甲狀腺素鈉片高劑量組5組,每組各18只,參照Feeney's自由落體打擊造模法復(fù)制動(dòng)物模型;于傷后6h灌胃。灌胃后24、72、168h分別通過(guò)TUNEL法、ELISA法及放射免疫法檢測(cè)神經(jīng)細(xì)胞凋亡、血清NSE、IL-6及血清FT3、FT4水平。結(jié)果 (1)重型顱腦損傷大鼠受傷后血清FT3、FT4水平低于正常水平,FT4在168h降到最低,甲狀腺素可使FT3、FT4水平升高。(2)重型顱腦損傷大鼠受傷后出現(xiàn)明顯的神經(jīng)細(xì)胞凋亡,這種凋亡持續(xù)至168h。中劑量及高劑量甲狀腺素在24h即可以改善神經(jīng)細(xì)胞凋亡,低劑量甲狀腺素在168h才可以改善神經(jīng)細(xì)胞凋亡。(3)重型顱腦損傷大鼠血清NSE、IL-6于傷后明顯升高,一直持續(xù)到168h。中劑量及高劑量甲狀腺素在72h即可以降低血清NSE、IL-6水平。結(jié)論外源性甲狀腺素對(duì)重型顱腦損傷大鼠腦組織具有保護(hù)作用。
[Abstract]:Objective to observe the effects of thyroxine on neuronal apoptosis, serum neuron-specific enolase (NSEN) and interleukin-6 (IL-6) in rats with severe craniocerebral injury. To investigate the protective effect of thyroxine on brain tissue of severe craniocerebral injury methods 90 SD rats were divided into control group and control group. Model group? There were 18 rats in each group in low dose group, middle dose group and high dose group. The animal model was made by Feeney's free fall method. Neuronal apoptosis was detected by TUNEL Elisa and radioimmunoassay at 6 hours after injury and 2472168 hours after injury. Results the levels of serum FT 3 T 4 in rats with severe craniocerebral injury were lower than those in normal rats and the levels of FT 4 decreased to the lowest at 168 h after severe craniocerebral injury. Thyroxine could increase the level of FT3T4 in rats with severe craniocerebral injury and induce neuronal apoptosis, which lasted up to 168h.Thyroxine at medium and high doses could improve neuronal apoptosis in 24 hours. Low dose thyroxine could improve neuronal apoptosis in 168h) the serum NSE-IL-6 increased significantly in rats with severe craniocerebral injury after injury. Thyroxine at medium and high doses could decrease the serum NSEP IL-6 level at 72 h. Conclusion exogenous thyroxine has protective effect on brain tissue of rats with severe craniocerebral injury.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院;廣西醫(yī)科大學(xué)藥學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81460687) 廣西壯族自治區(qū)衛(wèi)生廳中醫(yī)藥科技專項(xiàng)(GZLC14-29)
【分類號(hào)】：R651.15

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本文編號(hào)：1647696