本文選題：膿毒癥　切入點：旋毛蟲　出處：《南方醫(yī)科大學學報》2016年08期 　論文類型：期刊論文

【摘要】：目的觀察旋毛蟲及其蟲源性蛋白對盲腸結扎穿孔(CLP)誘導的小鼠膿毒癥的影響。方法 80只雄性BALB/c小鼠隨機分為假手術組、CLP組、旋毛蟲肌幼蟲(ML)預感染組、旋毛蟲肌幼蟲蟲體可溶性蛋白(SMP)處理組和排泄分泌蛋白(MES)處理組。ML預感染組于術前28 d經口感染300條旋毛蟲肌幼蟲,其余各組分別于術后30 min腹腔注射PBS或SMP(25μg/只)或MES(25μg/只)。觀察小鼠術后狀態(tài)和72 h生存率,檢測術后12 h小鼠血清中丙氨酸氨基轉移酶(ALT)、天門冬氨酸氨基轉移酶(AST)、尿素氮(BUN)和肌酐(Cr)水平及TNF-α、IL-6、IL-1β、IL-10、TGF-β水平,觀察小鼠肝和腎組織病變。結果與假手術組相比,CLP組72 h生存率降低,血清中ALT、AST、BUN和Cr水平及細胞因子TNF-α、IL-6、IL-1β、IL-10和TGF-β水平均明顯升高(P0.05)。肝中肝索排列紊亂,肝細胞水腫,腎中部分血管球皺縮,腎小管細胞水腫。與CLP組相比,ML預感染組血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,IL-10和TGF-β水平升高(P0.05);SMP處理組血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,TGF-β水平升高(P0.05);MES處理組72 h生存率明顯升高,血清中ALT、AST、BUN、Cr、TNF-α、IL-6和IL-1β水平明顯降低,IL-10和TGF-β水平明顯升高(P0.05),肝和腎組織結構損傷明顯減輕。結論旋毛蟲及其蟲源性蛋白可減少CLP誘導的膿毒癥小鼠血清中促炎因子的釋放,促進免疫調節(jié)因子的釋放,其中MES效果更為顯著,并能減輕肝和腎結構和功能的損傷。
[Abstract]:Objective to observe the effect of Trichinella spiralis and its insect-derived protein on sepsis induced by cecal ligation and perforation in mice. Methods 80 male BALB/c mice were randomly divided into two groups: sham operation group and trichinella muscle larva preinfection group. The muscle larvae of Trichinella spiralis were treated with soluble protein (SMPP) and excretory and secretory protein (MESs). ML preinfection group was infected with 300 trichinella muscle larvae by mouth 28 days before operation. The other groups were injected intraperitoneally with PBS or SMP(25 渭 g / mouse or MES(25 渭 g / mouse at 30 min after operation. The postoperative state and 72 h survival rate of the mice were observed. The serum levels of alanine aminotransferase (alt), aspartate aminotransferase (ASTU), bun (bun), creatinine (Cr) and TNF- 偽 IL-6 尾 IL-10 TGF- 尾 in serum of mice were measured 12 hours after operation, and the pathological changes of liver and kidney were observed. Results the 72 h survival rate of CLP group was lower than that of sham CLP group. The levels of bun and Cr in serum and cytokines TNF- 偽, IL-6, IL-1 尾, IL-10 and TGF- 尾 in serum were significantly higher than those in control group (P 0.05). Renal tubular cell edema. Compared with CLP group, the serum levels of TNF- 偽 and TGF- 尾 in ML-preinfection group were decreased, the levels of IL-10 and TGF- 尾 were increased, the serum levels of TGF- 偽 and TGF- 尾 in serum of P0.05- SMPtreated group increased significantly, and the survival rate of TGF- 尾 increased significantly in P0.05MES treatment group. The levels of TNF- 偽 IL-6 and IL-1 尾 in serum of Asthma sinensis were significantly decreased, the levels of IL-10 and TGF- 尾 increased significantly, and the damage of liver and kidney tissue structure was alleviated. Conclusion Trichinella spiralis and its insect-derived protein can reduce the release of pro-inflammatory factor in serum of CLP induced sepsis mice. Promote the release of immunomodulators, MES is more effective, and can reduce the damage of liver and kidney structure and function.
【作者單位】： 蚌埠醫(yī)學院組織學與胚胎學教研室;蚌埠醫(yī)學院病原生物學教研室;蚌埠醫(yī)學院病理學教研室;蚌埠醫(yī)學院第二附屬醫(yī)院;蚌埠醫(yī)學院人體解剖學教研室;
【基金】：國家自然科學基金項目(81441120) 高�？蒲袆�(chuàng)新平臺團隊項目(2016-40) 安徽省自然科學基金項目(1508085QH158) 安徽省高校優(yōu)秀青年人才支持計劃重點項目(gxyq ZD2016159) 安徽省高等學校自然科學研究重點項目(KJ2016A708) 安徽省自然科學青年科學基金項目(1608085QC51) 安徽省高等學校自然科學研究項目(KJ2015B013by) 蚌埠醫(yī)學院自然科學研究重點項目(BYKL1402ZD) 安徽省級大學生創(chuàng)新項目(201510367016)~~
【分類號】：R459.7

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