發(fā)布時間：2018-02-25 23:29

  本文關鍵詞： 阿托伐他汀 膿毒癥 肺損傷 細胞炎性蛋白- Toll樣受體-　出處：《中國老年學雜志》2016年13期 　論文類型：期刊論文

【摘要】：目的探討阿托伐他汀對膿毒癥大鼠急性肺損傷(ALI)的保護作用及其相關機制。方法采用盲腸結扎穿孔(CLP)方法構建大鼠膿毒癥ALI模型,40只大鼠隨機分為假手術組(S組)、模型組(M組)、阿托伐他汀低劑量組(L組,20 mg/kg)和阿托伐他汀高劑量組(H組,40 mg/kg),阿托伐他汀治療組術后6 h和18 h腹腔注射不同劑量的阿托伐他汀,S、M組同時間給予等量的生理鹽水腹腔注射。術后20 h處死大鼠收集血液及肺組織標本。測定實驗各組動脈血氣指標、肺水含量、支氣管肺泡灌洗液中蛋白含量及細胞計數(shù);酶聯(lián)免疫吸附(ELISA)法檢測血清中細胞炎性蛋白(MIP)-2及Toll樣受體(TLR)-4水平;反轉錄PCR方法檢測肺組織MIP-2及TLR-4 mRNA的表達。結果與M組比較,動脈血氣指標、肺水含量、支氣管肺泡灌洗液中蛋白含量及細胞計數(shù)在L組、H組均顯著改善(P0.05),且H組改善更明顯(P0.05)。與M比較,阿托伐他汀干預組血清MIP-2及TLR-4水平和肺組織MIP-2及TLR-4 mRNA的表達均明顯降低(P0.05),且H組改善更明顯(P0.05)。結論阿托伐他汀對膿毒癥大鼠ALI具有保護作用,這種保護作用可能與其抑制MIP-2及TLR-4表達有關。
[Abstract]:Objective to investigate the protective effect of Atto vastatin on acute lung injury in septic rats and its related mechanism. Methods 40 rats with sepsis ALI model were established by cecal ligation and perforation. Group M, model group, low dose group of Atto vastatin, group L, 20 mg / kg) and Atto vastatin high dose group, group H, 40 mg / kg, Atto statin treatment group, intraperitoneal injection of different doses of Atto statin at 6 h and 18 h after operation were given at the same time. Rats were sacrificed 20 hours after operation to collect blood and lung tissue samples. Arterial blood gas indexes were measured in each group. The contents of lung water, protein content and cell count in bronchoalveolar lavage fluid (BALF) were determined by Elisa, and the levels of cellular inflammatory protein (CIP)-2 and Toll-like receptor (TLR- 4) in serum were detected by Elisa. The expression of MIP-2 and TLR-4 mRNA in lung tissue was detected by reverse transcription PCR. Results compared with group M, arterial blood gas index and lung water content were measured. The protein content and cell count in bronchoalveolar lavage fluid (BALF) were significantly improved in group L (P 0.05), and in group H (P 0.05). The levels of serum MIP-2 and TLR-4 and the expression of MIP-2 and TLR-4 mRNA in the lung tissue of the Atto vastatin intervention group were significantly lower than that of the control group. Conclusion Atto vastatin has protective effect on ALI in septic rats. This protective effect may be related to its inhibition of the expression of MIP-2 and TLR-4.
【作者單位】： 華中科技大學同濟醫(yī)學院附屬梨園醫(yī)院藥劑科;華潤武鋼總醫(yī)院藥械中心;
【分類號】：R459.7
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本文編號：1535619