Objective To investigate the roles of Rho/Rock signaling pathway in silica-induced Epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells (BEC) in vitro. Methods Human BEC were incubated with silica with various concentrations for indicated times. Cell viability was assayed by MTT test. Morphologic Changes were observed by microscope. Mesenchymal marker α-smooth muscle actin (α-SMA), vimentin (Vim), and epithelial marker E-cadherin (E-cad) were analyzed by Western Blot. The ...

【文章頁數(shù)】：6 頁

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Preparation of Crystalline Silica
    Cell Culture and Reagents
    MTT Assay
    Western Blot Analysis
    Rho Activation Assay
    Statistical Method
RESULTS
    Effects of Silica on the Viability and Morphology of Human BEC
    Effects of Silica on the Expression of E-cad, α-SMA, and Vim of Human BEC
    Effects of Silica on Rho Activity of Human BEC
    Effects of Rho/Rock Inhibitor on Silica-induced EMT of Human BEC
DISCUSSION



本文編號：3853315