目的探討miRNA-21對(duì)大鼠骨髓間充質(zhì)干細(xì)胞（bone marrow mesenchymal stem cells,BMSCs）特性的影響。方法 BMSCs使用DMEM液進(jìn)行培養(yǎng)傳代,流式細(xì)胞儀檢測(cè)BMSCs的表面相關(guān)抗原表達(dá)。建立miRNA-21慢病毒干擾載體（LV-miRNA-21）,并轉(zhuǎn)染BMSCs。分3組細(xì)胞進(jìn)行對(duì)比實(shí)驗(yàn)[CK（正常組）、NC（對(duì)照組）、miRNA-21組],分別培養(yǎng)3 d、7 d和14 d;利用免疫熒光染色技術(shù)和Western-blotting技術(shù)檢測(cè)并比較肌鈣蛋白在3組細(xì)胞中3個(gè)時(shí)間點(diǎn)的表達(dá)情況,噻唑藍(lán)（MTT）法檢測(cè)3組BMSCs的增殖情況,運(yùn)用流式細(xì)胞技術(shù)檢測(cè)3組BMSCs的細(xì)胞周期及凋亡情況。結(jié)果 BMSCs呈長(zhǎng)梭形不規(guī)則形態(tài),表現(xiàn)出干細(xì)胞特異性抗原CD90和CD29陽(yáng)性,CD45陰性。成功構(gòu)建LV-miRNA-21;發(fā)現(xiàn)在miRNA-21組BMSCs中經(jīng)誘導(dǎo)分化后肌鈣蛋白表達(dá)量明顯高于CK組和NC組（P<0.05）,在miRNA-21組中表現(xiàn)出細(xì)胞的增殖加快,但凋亡速度比CK組和NC組減慢。結(jié)論 miRNA-21影響B(tài)MSCs的增殖和凋亡,可... 

【文章來(lái)源】：中國(guó)胸心血管外科臨床雜志. 2020,27(08)CSCD

【文章頁(yè)數(shù)】：8 頁(yè)

【參考文獻(xiàn)】：
期刊論文
[1]Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts[J]. Maya Fakhry,René Buchet,David Magne,Eva Hamade,Bassam Badran.  World Journal of Stem Cells. 2013(04)
[2]Differentiation of bone marrow mesenchymal stem cells induced by myo-cardial medium under hypoxic conditions[J]. Xiao-jie XIE~1 Jian-an WANG~(1,3)Jiang CAO~2 Xing ZHANG~2 1 Division of Cardiology,the 2nd Affiliated Hospital,College of Medicine,Zhejiang University Hangzhou 310009,China;2 Clinical Research Institute,Sir Run Run Shaw Hospital,College of Medicine,Zhejiang University,Hangzhou 310016,China.  Acta Pharmacologica Sinica. 2006(09)



本文編號(hào)：3259615