【摘要】：目的初步探討RANKL和M-CSF在CIA大鼠關節(jié)滑膜的表達及T-614、MTX的干預作用。方法通過雞Ⅱ型膠原和完全弗氏佐劑混合的膠原乳劑免疫大鼠,建立CIA大鼠模型,將CIA大鼠隨機分為模型對照組、T-614組、MTX組和T-614+MTX組。藥物干預組大鼠分別用T-614、MTX及T-614聯(lián)合MTX干預3周。關節(jié)炎指數(shù)(arthritis index,AI)評分評價關節(jié)炎程度,HE染色觀察踝關節(jié)組織形態(tài)學變化,X線片觀察骨侵蝕情況,高頻超聲觀察踝關節(jié)并測量滑膜厚度,Western blot法檢測滑膜RANKL和M-CSF表達。結果與正常對照組相比,模型對照組AI評分、病理評分、X線評分、超聲下滑膜厚度、關節(jié)滑膜RANKL,M-CSF表達均增加(P0.05);與模型對照組相比,T-614組、MTX組、T-614聯(lián)合MTX組上述觀察指標均降低(P0.05);與T-614組、MTX組相比,T-614聯(lián)合MTX組上述觀察指標均降低(P0.05);T-614組和MTX組之間上述觀察指標差異無統(tǒng)計學意義(P0.05)。結論 RANKL和M-CSF參與了CIA大鼠關節(jié)滑膜病變的發(fā)生、發(fā)展,T-614、MTX可能通過減少細胞因子RANKL,M-CSF的生成來延緩關節(jié)滑膜病變的進展,T-614與MTX具有一定的協(xié)同效應。
[Abstract]:Objective To study the expression of RANKL and M-CSF in the joint synovium of CIA rats and the intervention of T-614 and MTX. Methods The CIA rats were randomly divided into the model control group, the T-614 group, the MTX group and the T-614 + MTX group by using the collagen emulsion mixed with the chicken type II collagen and the complete Freund's adjuvant to immunize the rat. The rats of the drug intervention group were treated with T-614, MTX and T-614 in combination with MTX for 3 weeks. Arthritis index (AI) score was used to evaluate the degree of arthritis, and the morphological changes of the ankle tissue were observed with HE staining. The bone erosion was observed by X-ray. The thickness of the synovium was measured by high-frequency ultrasound, and the expression of RANKL and M-CSF was detected by Western blot. Results Compared with the normal control group, the AI score, the pathological score, the X-ray score, the ultrasonic glide film thickness, the joint synovial RANKL and the M-CSF expression in the model control group were all increased (P0.05), and the above-mentioned observation indexes of the T-614 group, the MTX group and the T-614 combined MTX group were all decreased (P0.05). Compared with the T-614 group and the MTX group, the above-mentioned observation index of the T-614 combined MTX group was decreased (P0.05), and the difference of the observation indexes between the T-614 group and the MTX group was not statistically significant (P0.05). Conclusion RANKL and M-CSF are involved in the development, development, T-614 and MTX of the joint synovium of CIA rats.
【作者單位】： 東南大學醫(yī)學院;東南大學附屬中大醫(yī)院風濕免疫科;
【基金】：江蘇省自然基金(BK2009276)~~
【分類號】：R392.3

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