【摘要】：目前對(duì)NIRF(Np95/ICBP-90 like RING finger protein)的研究正朝著細(xì)胞癌變進(jìn)程以及表觀遺傳學(xué)的方向發(fā)展,但在體內(nèi)NIRF對(duì)乙型肝炎病毒(HBV)的復(fù)制及表達(dá)的影響,目前尚不明確.通過高壓水動(dòng)力法轉(zhuǎn)染HBV小鼠模型,不同時(shí)間點(diǎn)收集血液和肝組織標(biāo)本,熒光定量PCR檢測(cè)血清及肝組織中病毒載量,WesternBlot檢測(cè)肝組織HBc(hepatitis B virus core protein)表達(dá),ELISA檢測(cè)血清HBeAg表達(dá),并通過免疫組化染色檢測(cè)HBsAg、HBcAg在肝組織中的定位及表達(dá).小鼠轉(zhuǎn)染pAAV-HBV1.3和NIRF以后,血清及肝組織病毒載量降低(n=3,P0.05),HBc蛋白及HBV相關(guān)抗原的表達(dá)受到抑制,說明在水動(dòng)力法轉(zhuǎn)染HBV小鼠模型中NIRF對(duì)HBV的復(fù)制及表達(dá)起到抑制作用,期待能為后續(xù)的HBV致病機(jī)理及治療藥物的研究與開發(fā)提供支持與幫助.
[Abstract]:At present, the study of NIRF (Np95/ICBP-90 like RING finger protein) is developing towards the process of cell carcinogenesis and epigenetics, but the effect of NIRF on the replication and expression of hepatitis B virus (HBV) in vivo is not clear at present. The blood and liver tissue samples were collected at different time points. The viral load in serum and liver tissue was detected by fluorescence quantitative PCR, the expression of HBc (hepatitis B virus core protein) in liver tissue was detected by WesternBlot, and the expression of HBeAg in serum was detected by ELISA. The localization and expression of HBsAg,HBcAg in liver tissue were detected by immunohistochemical staining. After pAAV-HBV1.3 and NIRF were transferred into mice, the viral load in serum and liver tissue decreased (n 鈮，

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